Establishment of stable Huh-7 cell lines expressing various hepatitis C virus genotype 3a protein: an in-vitro testing system for novel anti-HCV drugs

ABSTRACT The hepatitis C virus (HCV) genotype 2a subgenomic replicon can replicate in two human non-hepatocyte-derived cell lines, HeLa and 293, with in vitro-transcribed replicon RNA. Sequencing analysis revealed that mutations in HCV-derived regions were not essential for replication in these cells, as some clones displayed no mutations.

Download Full-text

An in vitro model of hepatitis C virus genotype 3a-associated triglycerides accumulation

Journal of Hepatology ◽

10.1016/j.jhep.2004.12.034 ◽

2005 ◽

Vol 42 (5) ◽

pp. 744-751 ◽

Cited By ~ 117

Author(s):

Karim Abid ◽

Valerio Pazienza ◽

Andrea de Gottardi ◽

Laura Rubbia-Brandt ◽

Beatrice Conne ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

In Vitro Model ◽

Virus Genotype ◽

Vitro Model ◽

Genotype 3A

Download Full-text

Examination of the Activity of Camel Milk Casein against Hepatitis C Virus (Genotype-4a) and Its Apoptotic Potential in Hepatoma and HeLa Cell Lines

Hepatitis Monthly ◽

10.5812/kowsar.1735143x.722 ◽

2011 ◽

Vol 11 (9) ◽

Cited By ~ 4

Author(s):

Osama Almahdy ◽

Esmail M. EL-Fakharany ◽

Ehab EL-Dabaa ◽

Tzi Bun Ng ◽

Elrashdy M. Redwan

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Hela Cell ◽

Cell Lines ◽

Camel Milk ◽

Virus Genotype ◽

Hela Cell Lines ◽

Milk Casein

Download Full-text

Screening and identification of bioactive compounds from citrus against non-structural protein 3 protease of hepatitis C virus genotype 3a by fluorescence resonance energy transfer assay and mass spectrometry

World Journal of Hepatology ◽

10.4254/wjh.v12.i11.976 ◽

2020 ◽

Vol 12 (11) ◽

pp. 976-992

Author(s):

Mahim Khan ◽

Waqar Rauf ◽

Fazal-e- Habib ◽

Moazur Rahman ◽

Mazhar Iqbal

Keyword(s):

Mass Spectrometry ◽

Energy Transfer ◽

Hepatitis C Virus ◽

Hepatitis C ◽

Resonance Energy Transfer ◽

Resonance Energy ◽

Structural Protein ◽

Virus Genotype ◽

Screening And Identification ◽

Genotype 3A

Download Full-text

Quantitation of hepatitis C virus RNA production in two human bone marrow-derived B-cell lines infected in vitro

Research in Virology ◽

10.1016/s0923-2516(97)89901-3 ◽

1997 ◽

Vol 148 (2) ◽

pp. 147-151 ◽

Cited By ~ 16

Author(s):

S. Iacovacci ◽

L. Bertolini ◽

A. Manzin ◽

M.B. Valli ◽

M. Battaglia ◽

...

Keyword(s):

Bone Marrow ◽

Hepatitis C Virus ◽

Hepatitis C ◽

B Cell ◽

Cell Lines ◽

Human Bone ◽

Human Bone Marrow ◽

Hepatitis C Virus Rna ◽

Virus Rna

Download Full-text

Virological characterization of the hepatitis C virus JFH-1 strain in lymphocytic cell lines

Journal of General Virology ◽

10.1099/vir.0.83618-0 ◽

2008 ◽

Vol 89 (7) ◽

pp. 1587-1592 ◽

Cited By ~ 15

Author(s):

Kyoko Murakami ◽

Toshiro Kimura ◽

Motonao Osaki ◽

Koji Ishii ◽

Tatsuo Miyamura ◽

...

Keyword(s):

Hepatitis C Virus ◽

Hepatitis C ◽

Cell Lines ◽

Entry Site ◽

Internal Ribosome Entry ◽

Culture Systems ◽

Polyprotein Processing ◽

Lymphocytic Cell

While hepatocytes are the major site of hepatitis C virus (HCV) infection, a number of studies have suggested that HCV can replicate in lymphocytes. However, in vitro culture systems to investigate replication of HCV in lymphocytic cells are severely limited. Robust HCV culture systems have been established using the HCV JFH-1 strain and Huh-7 cells. To gain more insights into the tissue tropism of HCV, we investigated the infection, replication, internal ribosome entry site (IRES)-dependent translation and polyprotein processing of the HCV JFH-1 strain in nine lymphocytic cell lines. HCV JFH-1 failed to infect lymphocytes and replicate, but exhibited efficient polyprotein processing and IRES-dependent translation in lymphocytes as well as in Huh-7 cells. Our results suggest that lymphocytic cells can support HCV JFH-1 translation and polyprotein processing, but may lack some host factors essential for HCV JFH-1 infection and replication.

Download Full-text