scholarly journals A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification

2010 ◽  
Vol 7 (1) ◽  
pp. 14 ◽  
Author(s):  
Yang JinLong ◽  
Yang Rui ◽  
Cheng AnChun ◽  
Wang MingShu ◽  
Fu LiZhi ◽  
...  
Keyword(s):  
Rapid Method ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Goose Parvovirus

Development of a Rapid Method for the Visible Detection of Pork DNA in Halal Products by Loop-Mediated Isothermal Amplification

2015 ◽  
Vol 9 (3) ◽  
pp. 565-570 ◽  
Author(s):  
Guangyao Ran ◽  
Linzhu Ren ◽  
Xiaolei Han ◽  
Xingxing Liu ◽  
Zhuang Li ◽  
...  
Keyword(s):  
Rapid Method ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

scholarly journals Loop-Mediated Isothermal Amplification as a Simple Molecular Method for the Detection of Derzsy’s Disease Virus

2013 ◽  
Vol 57 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Karolina Tarasiuk ◽  
Grzegorz Woźniakowski ◽  
Elżbieta Samorek-Salamonowicz
Keyword(s):  
Disease Virus ◽  
Isothermal Amplification ◽  
Optimum Temperature ◽  
Molecular Method ◽  
Specific Primers ◽  
Loop Mediated Isothermal Amplification ◽  
Specific Assay ◽  
Goose Parvovirus

Abstract The objective of the study was to develop a simple and rapid molecular method for the detection of GPV. Twenty seven goose parvovirus (GPV) isolates collected from geese flocks in Poland were examined. Three pairs of specific primers: two outer primers (F3 and B3), two inner primers (FIP and BIP), and two loop primers (FL and BL) were used to accelerate the reaction. The optimum temperature and time of the reaction were 60°C and 30 min. The sensitivity of the method was 10-times higher than PCR. The method proved to be a sensitive, rapid, and specific assay for detecting GPV.

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