scholarly journals Loop-Mediated Isothermal Amplification as a Simple Molecular Method for the Detection of Derzsy’s Disease Virus

2013 ◽  
Vol 57 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Karolina Tarasiuk ◽  
Grzegorz Woźniakowski ◽  
Elżbieta Samorek-Salamonowicz
Keyword(s):  
Disease Virus ◽  
Isothermal Amplification ◽  
Optimum Temperature ◽  
Molecular Method ◽  
Specific Primers ◽  
Loop Mediated Isothermal Amplification ◽  
Specific Assay ◽  
Goose Parvovirus

Abstract The objective of the study was to develop a simple and rapid molecular method for the detection of GPV. Twenty seven goose parvovirus (GPV) isolates collected from geese flocks in Poland were examined. Three pairs of specific primers: two outer primers (F3 and B3), two inner primers (FIP and BIP), and two loop primers (FL and BL) were used to accelerate the reaction. The optimum temperature and time of the reaction were 60°C and 30 min. The sensitivity of the method was 10-times higher than PCR. The method proved to be a sensitive, rapid, and specific assay for detecting GPV.

scholarly journals Loop-Mediated Isothermal Amplification for Detection ofStaphylococcus aureusin Dairy Cow Suffering from Mastitis

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Zhang Tie ◽  
Wang Chunguang ◽  
Wei Xiaoyuan ◽  
Zhao Xinghua ◽  
Zhong Xiuhui
Keyword(s):  
Staphylococcus Aureus ◽  
High Sensitivity ◽  
High Specificity ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Specific Primers ◽  
Loop Mediated Isothermal Amplification ◽  
Reaction Tube ◽  
Specificity And Sensitivity ◽  
Detectable Concentration

To develop a rapid detection method ofStaphylococcus aureususing loop-mediated isothermal amplification (LAMP), four specific primers were designed according to six distinct sequences of thenucgene. In addition, the specificity and sensitivity of LAMP were verified and compared with those of PCR. Results showed that the LAMP reaction was completed within 45 min at 62.5°C, and ladder bands were appeared in LAMP products analyzed by gel electrophoresis. After adding 1x SYBR Green l, the positive reaction tube showed green color and the negative reaction tube remained orange, indicating that the LAMP has high specificity. The minimal detectable concentration of LAMP was1×102 CFU/mL and that of PCR was1×104 CFU/mL, indicating that the LAMP was 100 times more sensitive than the PCR. The LAMP method for detection ofStaphylococcus aureushas many advantages, such as simple operation, high sensitivity, high specificity, and rapid analysis. Therefore, this method is more suitable for the rapid on-site detection ofStaphylococcus aureus.

scholarly journals Rapid Newcastle Disease Virus Detection Based on Loop-Mediated Isothermal Amplification and Optomagnetic Readout

ACS Sensors ◽  
2016 ◽  
Vol 1 (10) ◽  
pp. 1228-1234 ◽  
Author(s):  
Bo Tian ◽  
Jing Ma ◽  
Teresa Zardán Gómez de la Torre ◽  
Ádám Bálint ◽  
Marco Donolato ◽  
...  
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Virus Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification

scholarly journals A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification

2010 ◽  
Vol 7 (1) ◽  
pp. 14 ◽  
Author(s):  
Yang JinLong ◽  
Yang Rui ◽  
Cheng AnChun ◽  
Wang MingShu ◽  
Fu LiZhi ◽  
...  
Keyword(s):  
Rapid Method ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Goose Parvovirus
Sign in / Sign up

Export Citation Format

Share Document