scholarly journals Bulk milk ELISA and the diagnosis of parasite infections in dairy herds: a review

2013 ◽  
Vol 66 (1) ◽  
pp. 14 ◽  
Author(s):  
Mary Sekiya ◽  
Annetta Zintl ◽  
Michael L Doherty
2012 ◽  
Vol 50 (No. 12) ◽  
pp. 549-552 ◽  
Author(s):  
L. Hurkova ◽  
D. Halova ◽  
D. Modry

Neospora caninum is a protozoan parasite causing bovine abortion all over the world. The aim of this study was to investigate how common N. caninum infection is among dairy herds in the Czech Republic. Bulk milk samples were collected from 495 dairy herds and analysed for the presence of specific antibodies by a commercial N. caninum iscom ELISA. Five out of 495 dairy herds (1.01%) had percentage positive values ≥20 and were considered positive. In the positive herds, blood samples were collected from cows and pregnant heifers and analysed by the ELISA test. The within-herd seroprevalence ranged from 2.5 to 50%. The bulk milk ELISA could be a useful and inexpensive method for rapid screening of Neospora caninum infection in dairy herds in large areas.


2009 ◽  
Vol 166 (3-4) ◽  
pp. 228-234 ◽  
Author(s):  
J.M. Molina ◽  
Y. Hernández ◽  
A. Ruiz ◽  
J.F. González ◽  
A. Argüello ◽  
...  

1999 ◽  
Vol 38 (2-3) ◽  
pp. 159-166 ◽  
Author(s):  
F.G. Wittwer ◽  
P. Gallardo ◽  
J. Reyes ◽  
H. Opitz

1984 ◽  
Vol 57 (2) ◽  
pp. 317-323 ◽  
Author(s):  
A.J. Bramley ◽  
C.H. McKinnon ◽  
R.T. Staker ◽  
D.L. Simpkin

1971 ◽  
Vol 34 (11) ◽  
pp. 517-520 ◽  
Author(s):  
D. S. Postle ◽  
R. P. Natzke ◽  
R. W. Everett

Bulk tank milk samples and quarter samples from 15,982 cows were collected from 285 herds enrolled in the New York State Mastitis Control Program. Culture and strip-plate examination findings from quarter samples were correlated with bulk milk cell counts. A correlation of 0.50 was found between percent quarters yielding mastitis pathogens and bulk milk cell counts. A correlation of 0.51 was found between percent quarters producing abnormal secretions and bulk milk cell counts. These relationships indicated the bulk milk cell count was not a reliable measure of herd infection or prevalence of abnormal secretion. The most prominent trend in a relationship between pathogens and increased bulk milk cell count was found for Streptococcus agalactiae and a lesser relationship between staphylococci and increased bulk milk cell count. Herds classified as having low bulk milk cell counts produced an average of 1,759 lb. of milk more than herds with high bulk milk cell counts. Only three milking and management practices were found to be associated with bulk milk cell counts.


2015 ◽  
Vol 98 (6) ◽  
pp. 3741-3753 ◽  
Author(s):  
D.A. Shock ◽  
S.J. LeBlanc ◽  
K.E. Leslie ◽  
K. Hand ◽  
M.A. Godkin ◽  
...  

1998 ◽  
Vol 142 (8) ◽  
pp. 181-184 ◽  
Author(s):  
L. Van Wuijckhuise ◽  
J. Bosch ◽  
P. Franken ◽  
K. Frankena ◽  
A. R. W. Elbers

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