Evaluation of a bulk-milk ELISA test for the classification of herd-level bovine leukemia virus status

1997 ◽  
Vol 31 (3-4) ◽  
pp. 223-230 ◽  
Author(s):  
J.M. Sargeant ◽  
D.F. Kelton ◽  
S.W. Martin ◽  
E.D. Mann
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Elisa Test ◽  
Herd Level ◽  
Bulk Milk ◽  
Milk Elisa ◽  
Bulk Milk Elisa

scholarly journals The prevalence of Neospora caninum antibodies in bulk milk of dairy herds in the CzechRepublic: a case report

2012 ◽  
Vol 50 (No. 12) ◽  
pp. 549-552 ◽  
Author(s):  
L. Hurkova ◽  
D. Halova ◽  
D. Modry
Keyword(s):  
Neospora Caninum ◽  
Protozoan Parasite ◽  
Elisa Test ◽  
Rapid Screening ◽  
Dairy Herds ◽  
Caninum Infection ◽  
Bulk Milk ◽  
Milk Elisa ◽  
Bovine Abortion ◽  
Bulk Milk Elisa

Neospora caninum is a protozoan parasite causing bovine abortion all over the world. The aim of this study was to investigate how common N. caninum infection is among dairy herds in the Czech Republic. Bulk milk samples were collected from 495 dairy herds and analysed for the presence of specific antibodies by a commercial N. caninum iscom ELISA. Five out of 495 dairy herds (1.01%) had percentage positive values ≥20 and were considered positive. In the positive herds, blood samples were collected from cows and pregnant heifers and analysed by the ELISA test. The within-herd seroprevalence ranged from 2.5 to 50%. The bulk milk ELISA could be a useful and inexpensive method for rapid screening of Neospora caninum infection in dairy herds in large areas.

Preliminary study on the use of a Teladorsagia circumcincta bulk milk ELISA test in dairy goats under experimental conditions

2009 ◽  
Vol 166 (3-4) ◽  
pp. 228-234 ◽  
Author(s):  
J.M. Molina ◽  
Y. Hernández ◽  
A. Ruiz ◽  
J.F. González ◽  
A. Argüello ◽  
...  
Keyword(s):  
Elisa Test ◽  
Dairy Goats ◽  
Experimental Conditions ◽  
Teladorsagia Circumcincta ◽  
Bulk Milk ◽  
Milk Elisa ◽  
Preliminary Study ◽  
Bulk Milk Elisa

scholarly journals Bayesian Estimation of the True Seroprevalence and Risk Factor Analysis of Bovine Leukemia Virus Infection in Pakistan

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1404
Author(s):  
Ali Sakhawat ◽  
Marzena Rola-Łuszczak ◽  
Zbigniew Osiński ◽  
Nazia Bibi ◽  
Jacek Kuźmak
Keyword(s):  
Risk Factor ◽  
Dairy Cattle ◽  
Bovine Leukemia Virus ◽  
Water Buffalo ◽  
Leukemia Virus ◽  
Credible Interval ◽  
Indigenous Cattle ◽  
Herd Level ◽  
Exotic Breed ◽  
Posterior Estimate

The objective of this study was to determine the true seroprevalence of bovine leukemia virus (BLV) infection in dairy cattle from Pakistan at the animal and herd-level. We tested 1380 dairy cattle from 451 herds and 92 water buffalo. The sera were tested by ELISA and the results were analyzed using Bayesian inference. The median posterior estimate of the herd level true BLV prevalence was 1.4%, with a 95% credible interval (CI) 0.7–3.1, whereas the median posterior estimate of the within-farm true seroprevalence was 3.8% with a 95% CI 2.8–4.8. All 92 sera collected from water buffalo were negative. Several risk factors potentially associated with seropositivity to BLV infections in Pakistan were analyzed using logistic regression model based on calculation of an odds ratio (OR). The study showed an association between seropositivity and medium herd (≥50) size (OR = 23.57, 95% CI: 3.01–103.48). Common housing of indigenous cattle with exotic-breed cattle (OR = 0.67, 95% CI: 06–2.35) or housing indigenous or their crossbred cattle with exotic-breed cattle (OR = 0.95, 95% CI: 0.14–3.01) had no effect on the BLV seroprevalence. Similarly, common housing of cattle and water buffalo was not risk factor for increased BLV seropositivity (OR = 27.10, 95% CI: 0.63–119.34).

scholarly journals Repeatability of a Commercially Available ELISA Test for Determining the Herd-Level Salmonella enterica subsp. enterica Serovar Dublin Status in Dairy Herds Using Bulk Milk

2020 ◽  
Vol 7 ◽  
Author(s):  
Maryse Michèle Um ◽  
Marie-Hélène Castonguay ◽  
Khalie Mahamad Amine ◽  
Julie Giguère ◽  
Isabelle Morin ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Elisa Test ◽  
Dairy Herds ◽  
Herd Level ◽  
Bulk Milk

scholarly journals Using a Herd Profile to Determine Age-Specific Prevalence of Bovine Leukemia Virus in Michigan Dairy Herds

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Ronald J. Erskine ◽  
Paul C. Bartlett ◽  
Todd M. Byrem ◽  
Chelsea L. Render ◽  
Catherine Febvay ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Leukemia Virus ◽  
Malignant Neoplasm ◽  
Dairy Herd ◽  
Dairy Herds ◽  
Management Plans ◽  
Milk Elisa ◽  
The Mean ◽  
Bovine Leukosis ◽  
Simple Average

Enzootic bovine leukosis is a contagious disease of cattle caused by the retrovirus, bovine leukemia virus (BLV) and is the most common cause of malignant neoplasm in cattle. In order to facilitate surveillance of this disease in dairy herds, we developed a method to combine ELISA of milk collected during routine production testing with a prescribed sampling of cows that is independent of the proportion of cows within each lactation. In 113 Michigan dairy herds, milk samples from ten cows in each of the 1st, 2nd, 3rd, and ≥4th lactations were analyzed for anti-Bovine Leukemia Virus (BLV) antibodies by milk ELISA. For each herd, a BLV herd profile (BHP) was calculated as the simple average of the percent of BLV-positive cows within each of the four lactation groups. The mean BHP for all herds was 32.8%, with means of 18.5, 28.8, 39.2, and 44.8% of 1st, 2nd, 3rd, and ≥4th lactation animals infected, respectively. In eight herds, we determined the correlation between the BHP, and true herd prevalence by testing the entire lactating herd (r=0.988,  P<0.0001). The BHP allows discrimination of lactation-specific BLV prevalence within a dairy herd, to help identify risk factors and management plans that may be important in transmission of BLV.

High herd-level seroprevalence and associated factors for bovine leukemia virus in the semi-arid Paraíba state, Northeast Region of Brazil

2021 ◽  
Vol 190 ◽  
pp. 105324
Author(s):  
Gisele Cândida Ramalho ◽  
Maria Luana Cristiny Rodrigues Silva ◽  
Brunna Muniz Rodrigues Falcão ◽  
Clécio Henrique Limeira ◽  
Denise Batista Nogueira ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Associated Factors ◽  
Leukemia Virus ◽  
Northeast Region ◽  
Herd Level ◽  
Semi Arid

scholarly journals Sero-prevalence of bovine Leukemia virus in cattle from Caquetá state, Colombia

2019 ◽  
Vol 11 (2) ◽  
pp. 722
Author(s):  
Pablo Andrés Motta-Delgado ◽  
Luis Gabriel Rivera-Calderón ◽  
Wilmer Herrera-Valencia ◽  
Ricardo Alberto Martínez-Tovar ◽  
Marliyanini Londoño-Sánchez ◽  
...  
Keyword(s):  
Bovine Leukemia Virus ◽  
Indirect Elisa ◽  
Leukemia Virus ◽  
Elisa Test ◽  
Dual Purpose ◽  
Positive Ratio ◽  
Dual Purpose Cattle ◽  
Bovine Leukosis ◽  
Bovine Species ◽  
Municipal Level

Bovine Leukemia Virus (BLV) is the agent of enzootic bovine leukosis (EBL), this disease is a neoplasm of lymphatic tissue in bovine species.  The aim of this article was determinate the prevalence of bovine leukemia virus in dual-purpose cattle from nine municipalities that produce the 98% of milk in the Caquetá state, Colombia. Blood samples were collected in 100 herds dedicates to dual purpose cattle, obtained blood serum from 1000 animals, of which 893 corresponding to cows. Indirect Elisa test for detection of antibodies anti-GP51 of BLV was performance and the positive cases were considered if the serum-to-positive ratio with percentages of M/N lower than 40%. Categorized data were analyzed by contingency tables and ANOVA at the significant level of p<0.05 by DGC test was performed. The overall sero-prevalence of BLV in Caquetá state was of 25.18% (95%, CI: 21.9-28.46%), in males 26.25% and females 25.37% respectively. At municipal level the sero-prevalence varied of 7.12 to 41.81%. The prevalence of BLV at herd level was of 67% (95%, CI: 57.24-76.76%). In conclusion, the sero-prevalence of BLV in the dual-purpose livestock system over 36 months of age in Caquetá state is moderate, do not exist statistical difference between sero-prevalence of cows and bulls. At level of herds the prevalence of BLV is high. Improving strategies of control and managements in the herds, as well as implement policies of sanitary management are necessary.

scholarly journals Bulk milk ELISA and the diagnosis of parasite infections in dairy herds: a review

2013 ◽  
Vol 66 (1) ◽  
pp. 14 ◽  
Author(s):  
Mary Sekiya ◽  
Annetta Zintl ◽  
Michael L Doherty
Keyword(s):  
Dairy Herds ◽  
Bulk Milk ◽  
Parasite Infections ◽  
Milk Elisa ◽  
Bulk Milk Elisa
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