scholarly journals The prevalence of Neospora caninum antibodies in bulk milk of dairy herds in the CzechRepublic: a case report

2012 ◽  
Vol 50 (No. 12) ◽  
pp. 549-552 ◽  
Author(s):  
L. Hurkova ◽  
D. Halova ◽  
D. Modry
Keyword(s):  
Neospora Caninum ◽  
Protozoan Parasite ◽  
Elisa Test ◽  
Rapid Screening ◽  
Dairy Herds ◽  
Caninum Infection ◽  
Bulk Milk ◽  
Milk Elisa ◽  
Bovine Abortion ◽  
Bulk Milk Elisa

Neospora caninum is a protozoan parasite causing bovine abortion all over the world. The aim of this study was to investigate how common N. caninum infection is among dairy herds in the Czech Republic. Bulk milk samples were collected from 495 dairy herds and analysed for the presence of specific antibodies by a commercial N. caninum iscom ELISA. Five out of 495 dairy herds (1.01%) had percentage positive values ≥20 and were considered positive. In the positive herds, blood samples were collected from cows and pregnant heifers and analysed by the ELISA test. The within-herd seroprevalence ranged from 2.5 to 50%. The bulk milk ELISA could be a useful and inexpensive method for rapid screening of Neospora caninum infection in dairy herds in large areas.

Use of bulk milk for detection of Neospora caninum infection in dairy herds in Thailand

2002 ◽  
Vol 110 (1-2) ◽  
pp. 35-44 ◽  
Author(s):  
Aran Chanlun ◽  
Katarina Näslund ◽  
Suneerat Aiumlamai ◽  
Camilla Björkman
Keyword(s):  
Neospora Caninum ◽  
Dairy Herds ◽  
Caninum Infection ◽  
Bulk Milk

Preliminary study on the use of a Teladorsagia circumcincta bulk milk ELISA test in dairy goats under experimental conditions

2009 ◽  
Vol 166 (3-4) ◽  
pp. 228-234 ◽  
Author(s):  
J.M. Molina ◽  
Y. Hernández ◽  
A. Ruiz ◽  
J.F. González ◽  
A. Argüello ◽  
...  
Keyword(s):  
Elisa Test ◽  
Dairy Goats ◽  
Experimental Conditions ◽  
Teladorsagia Circumcincta ◽  
Bulk Milk ◽  
Milk Elisa ◽  
Preliminary Study ◽  
Bulk Milk Elisa

scholarly journals Bulk milk ELISA and the diagnosis of parasite infections in dairy herds: a review

2013 ◽  
Vol 66 (1) ◽  
pp. 14 ◽  
Author(s):  
Mary Sekiya ◽  
Annetta Zintl ◽  
Michael L Doherty
Keyword(s):  
Dairy Herds ◽  
Bulk Milk ◽  
Parasite Infections ◽  
Milk Elisa ◽  
Bulk Milk Elisa

scholarly journals Evidence of Neospora caninum exposure among native Korean goats (Capra hircus coreanae)

2014 ◽  
Vol 59 (No. 12) ◽  
pp. 637-640 ◽  
Author(s):  
BY Jung ◽  
SH Lee ◽  
D. Kwak
Keyword(s):  
Neospora Caninum ◽  
Control Strategies ◽  
Integrated Control ◽  
Protozoan Parasite ◽  
Capra Hircus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Caninum Infection ◽  
One Year ◽  
And Control

Neospora caninum is a protozoan parasite that causes abortion in ruminants, including goats. The objective of the present study was to determine the seroprevalence of N. caninum in native Korean goats (Capra hircus coreanae). A commercial enzyme-linked immunosorbent assay kit was used to analyse 464 serum samples for the presence of N. caninum antibodies. Four samples (0.9%, 95% confidence intervals – CI: 0.0–1.7) were found to be positive for N. caninum antibodies. The seroprevalence was analysed according to age (less than to one year, young; more than or equal one year, adult; and unknown), sampling season (April to September, warm; October to March, cold), and region (northern, central, and southern). However, there were no statistically significant differences in seroprevalence according to age, season, and region (P > 0.05). This is the first report on the seroprevalence of N. caninum in native Korean goats. The results of this study indicate a nationwide distribution of N. caninum among goats, with a relatively low prevalence. Therefore, the implementation of integrated control strategies as well as measures for prevention and control of N. caninum infection among goats is recommended.

Epidemiology and Impact of Neospora caninum Infection in Three Queensland Tropical Dairy Herds

2010 ◽  
Vol 46 (4) ◽  
pp. 734-737 ◽  
Author(s):  
JK Landmann ◽  
AA Gunn ◽  
PJ O’Donoghue ◽  
WP Tranter ◽  
MR McGowan
Keyword(s):  
Neospora Caninum ◽  
Dairy Herds ◽  
Caninum Infection

Seroprevalence of Neospora caninum infection in dairy cows in Northern provinces, Thailand

2014 ◽  
Vol 59 (2) ◽  
Author(s):  
Tawin Inpankaew ◽  
Sathaporn Jittapalapong ◽  
Thomas Mitchell ◽  
Chainirun Sununta ◽  
Ikuo Igarashi ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Neospora Caninum ◽  
Fluorescent Antibody ◽  
Protozoan Parasite ◽  
Antibody Test ◽  
Current Status ◽  
Enzyme Linked Immunosorbent Assay ◽  
Caninum Infection ◽  
Slow Growth Rate ◽  
The Impact

AbstractNeospora caninum, an obligate intracellular protozoan parasite, is the causative agent of neosporosis, recognized as a major cause of bovine abortion around the world. Thailand is a developing agricultural country located in Southeast Asia. Livestock developments particularly in dairy cows of this country have been hampered by low productivity including milk and slow growth rate due to the impact of many pathogens including N. caninum. Currently, there is no effective method for control of neosporosis since there is less information regarding current status of infections. The objective of this study was to investigate the seroprevalence of neosporosis in dairy cows of the northern part of Thailand. During 2006–2007, the sera of 642 cows from 42 small farm holders with the top three highest consensus of dairy farms in the northern provinces, such as Chiang Rai, Chiang Mai and Lumpang were collected and performed tests. Antibodies to N. caninum were assayed by enzyme-linked immunosorbent assay (ELISA) with recombinant N. caninum surface antigen 1 (NcSAG1) and indirect fluorescent antibody test (IFAT). The overall prevalence of N. caninum infection in this study was 46.9% (301/642) by ELISA and 34.3% (220/642) by IFAT.

Prevalence of Neospora caninum infection in Sardinian dairy farms (Italy) detected by iscom ELISA on tank bulk milk

2005 ◽  
Vol 98 (3) ◽  
pp. 264-267 ◽  
Author(s):  
A. Varcasia ◽  
G. Capelli ◽  
A. Ruiu ◽  
M. Ladu ◽  
A. Scala ◽  
...  
Keyword(s):  
Neospora Caninum ◽  
Dairy Farms ◽  
Caninum Infection ◽  
Bulk Milk

scholarly journals Serological survey of Neospora caninum in small ruminants from Pernambuco State, Brazil

2011 ◽  
Vol 20 (3) ◽  
pp. 246-248 ◽  
Author(s):  
Antonio Amélia Santos Mucalane Tembue ◽  
Rafael Antonio de Nascimento Ramos ◽  
Thais Rabelo de Sousa ◽  
Alessandra Ribeiro Albuquerque ◽  
Alvimar José da Costa ◽  
...  
Keyword(s):  
Neonatal Mortality ◽  
Neospora Caninum ◽  
Small Ruminants ◽  
Antibody Test ◽  
Northeastern Brazil ◽  
Serological Survey ◽  
Caninum Infection ◽  
Bovine Abortion

Neospora caninum infection is an important cause of bovine abortion as well as neonatal mortality in goats and sheep. A serological survey for antibodies against N. caninum in goats and sheep was carried out in the municipality of Ibimirim, PE, Northeastern Brazil. The imunnofluoresce antibody test showed that 26.6% (85/319) of the goats and 64.2% (52/81) of the sheep were positive. Serologic reactivity was associated with age in goats (p < 0.01) and sheep (p > 0.05), with increasing rates in older animals. These results indicate exposure to N. caninum among small ruminants in the study area.

scholarly journals Seroprevalence of Neospora caninum in dairy cows in Belgrade city area, Serbia

2018 ◽  
Vol 69 (2) ◽  
pp. 979
Author(s):  
Đ. CVETOJEVIC ◽  
V. MILICEVIC ◽  
B. KURELJUSIC ◽  
B. SAVIC
Keyword(s):  
Dairy Cattle ◽  
Dairy Cows ◽  
Neospora Caninum ◽  
Protozoan Parasite ◽  
Positive Sample ◽  
High Seroprevalence ◽  
Caninum Infection ◽  
Elisa Kit ◽  
City Area ◽  
Epidemiological Factors

The protozoan parasite Neospora caninum is one of the most important abortifacient pathogen in cattle. Serological investigations are often used in order to estimate seroprevalence in herds. Aims of our study were to determine the seroprevalence among aborting and non-aborting dairy cattle in Belgrade city area as well as epidemiological factors that are important for N. caninum infection. Using commercial ELISA kit, we examined 188 sera. Out of 188, 142 samples originated from pregnant (non-aborting) cows from five farms, while 46 were from cows who had aborted. Overall seroprevalence was 25% (48/188). Seroprevalence was significantly higher (p≤0.05) in aborting than in non-aborting group of cows (37% and 21.1% respectively). At least one positive sample was detected on four (80%) out of five examined farms while seroprevalence among farms varied from 0 to 43.5%. On all examined farms crucial epizootiological factors (presence of dogs and low biosecurity measures) that favor the maintenance and spreading of the infection were identified. Our study revealed the presence of N. caninum antibodies in population of dairy cows in Belgrade city area. Infection is established in enzootic pattern on examined farms and high seroprevalence among aborting cows suggests that N. caninum could be important abortifacient pathogen.

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