Preliminary study on the use of a Teladorsagia circumcincta bulk milk ELISA test in dairy goats under experimental conditions

2009 ◽  
Vol 166 (3-4) ◽  
pp. 228-234 ◽  
Author(s):  
J.M. Molina ◽  
Y. Hernández ◽  
A. Ruiz ◽  
J.F. González ◽  
A. Argüello ◽  
...  
Keyword(s):  
Elisa Test ◽  
Dairy Goats ◽  
Experimental Conditions ◽  
Teladorsagia Circumcincta ◽  
Bulk Milk ◽  
Milk Elisa ◽  
Preliminary Study ◽  
Bulk Milk Elisa

scholarly journals The prevalence of Neospora caninum antibodies in bulk milk of dairy herds in the CzechRepublic: a case report

2012 ◽  
Vol 50 (No. 12) ◽  
pp. 549-552 ◽  
Author(s):  
L. Hurkova ◽  
D. Halova ◽  
D. Modry
Keyword(s):  
Neospora Caninum ◽  
Protozoan Parasite ◽  
Elisa Test ◽  
Rapid Screening ◽  
Dairy Herds ◽  
Caninum Infection ◽  
Bulk Milk ◽  
Milk Elisa ◽  
Bovine Abortion ◽  
Bulk Milk Elisa

Neospora caninum is a protozoan parasite causing bovine abortion all over the world. The aim of this study was to investigate how common N. caninum infection is among dairy herds in the Czech Republic. Bulk milk samples were collected from 495 dairy herds and analysed for the presence of specific antibodies by a commercial N. caninum iscom ELISA. Five out of 495 dairy herds (1.01%) had percentage positive values ≥20 and were considered positive. In the positive herds, blood samples were collected from cows and pregnant heifers and analysed by the ELISA test. The within-herd seroprevalence ranged from 2.5 to 50%. The bulk milk ELISA could be a useful and inexpensive method for rapid screening of Neospora caninum infection in dairy herds in large areas.

scholarly journals Effect of Infection with, and Treatment of, Sensitive and Resistant Strains of Teladorsagia Circumcincta on the Ovine Intestinal Microbiota

2018 ◽  
Author(s):  
Craig A. Watkins ◽  
Dave J. Bartley ◽  
Burcu Gündüz Ergün ◽  
Büşra Yıldızhan ◽  
Tracy Ross-Watt ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Anthelmintic Resistance ◽  
Predictive Biomarkers ◽  
Alpha Diversity ◽  
Nematode Species ◽  
Microbial Composition ◽  
Experimental Conditions ◽  
Teladorsagia Circumcincta ◽  
Resistant Strains ◽  
Preliminary Study

ABSTRACTNematodes are one of the main impactors on health, welfare and productivity of farmed animals. Teladorsagia circumcincta is arguably one of the most globally important nematode species in sheep. Control of these nematode infections is essential and heavily reliant on chemotherapy (anthelmintics), but this has been complicated by the development of anthelmintic resistance. In mammals the composition of the intestinal microbiota has been shown to have a significant effect on overall health. The interactions between host, microbiota and pathogens are complex and influenced by numerous factors. In this study, the interactions between T. circumcincta infections and microbial composition and abundance were investigated. In a preliminary study the intra-and inter-individual diversity and composition of the microbiota of grazing sheep was assessed in two distinct regions of the ovine intestinal tract, the terminal ileum (TI) and rectal contents. Additionally, the effects of experimental infection of sheep with two strains of T. circumcincta (anthelmintic resistant or sensitive) on the microbiota were assessed with and without anthelmintic (monepantel) administration. The inter-animal variability was greater in the TI compared to the rectal samples. However, the alpha-diversity (species richness) was significantly lower in the TI samples. In the experimental study, clear differences were observed between successfully treated animals and those sheep that were left untreated and/or those carrying resistant nematodes. Differences in microbiota between the four different experimental conditions were observed and potential predictive biomarkers were identified. In particular, a restoration of potentially beneficial Bifidobacteria sp. in successfully-treated animals was observed.

scholarly journals Bulk milk ELISA and the diagnosis of parasite infections in dairy herds: a review

2013 ◽  
Vol 66 (1) ◽  
pp. 14 ◽  
Author(s):  
Mary Sekiya ◽  
Annetta Zintl ◽  
Michael L Doherty
Keyword(s):  
Dairy Herds ◽  
Bulk Milk ◽  
Parasite Infections ◽  
Milk Elisa ◽  
Bulk Milk Elisa

A Preliminary Study on the Angular Distribution of Scattered Ultrasound from Bovine Liver and Myocardium

1987 ◽  
Vol 9 (2) ◽  
pp. 132-145
Author(s):  
Thomas M. Burke ◽  
Ernest L. Madsen ◽  
James A. Zagzebski
Keyword(s):  
Angular Distribution ◽  
Connective Tissue ◽  
Cross Section ◽  
Unit Volume ◽  
Bovine Liver ◽  
Experimental Conditions ◽  
Ultrasonic Scattering ◽  
Differential Scattering Cross Section ◽  
Scattering Cross ◽  
Preliminary Study

Measurements were performed on freshly-excised bovine liver and myocardium to determine the ultrasonic scattering nature of the tissues under a variety of experimental conditions. Results for the angular distribution of the differential scattering cross section per unit volume of tissue are reported for scattering angles spanning 170 to 44 degrees for interrogating frequencies of 1.0, 2.25, 3.5 and 5.0 MHz. Fresh and aged tissues, some with abnormally high connective tissue content were analyzed. The results are compared to previously-published works.

Development of a milk and serum ELISA test for the detection of Teladorsagia circumcincta antibodies in goats using experimentally and naturally infected animals

2014 ◽  
Vol 113 (10) ◽  
pp. 3651-3660 ◽  
Author(s):  
Eleni Malama ◽  
Peggy Hoffmann-Köhler ◽  
Insa Biedermann ◽  
Regine Koopmann ◽  
Jürgen Krücken ◽  
...  
Keyword(s):  
Elisa Test ◽  
Teladorsagia Circumcincta ◽  
Serum Elisa

scholarly journals Interactions between Teladorsagia circumcincta Infections and Microbial Composition of Sheep with or without Successful Monepantel Treatment—A Preliminary Study

Ruminants ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 31-45
Author(s):  
Craig A. Watkins ◽  
Dave J. Bartley ◽  
Burcu Gündüz Ergün ◽  
Büşra Yıldızhan ◽  
Tracy Ross-Watt ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Terminal Ileum ◽  
Anthelmintic Resistance ◽  
Economic Losses ◽  
Faecal Microbiota ◽  
Microbial Composition ◽  
Teladorsagia Circumcincta ◽  
Faecal Samples ◽  
Resistant Strains ◽  
Preliminary Study

Nematodes are one of the main impactors on the health, welfare and productivity of farmed animals. Teladorsagia circumcincta are endemic throughout many sheep-producing countries, particularly in the northern hemisphere, and contribute to the pathology and economic losses seen on many farms. Control of these nematode infections is essential and heavily reliant on chemotherapy (anthelmintics), but this has been compromised by the development of anthelmintic resistance. In mammals, the composition of the intestinal microbiota has been shown to have a significant effect on overall health. The interactions between host, microbiota and pathogens are complex and influenced by numerous factors. In this study, comparisons between intestinal and faecal microbiota of sheep infected with sensitive or resistant strains of T. circumcincta, with or without monepantel administration were assessed. The findings from both faecal samples and terminal ileum mucosal scrapings showed clear differences between successfully treated animals and those sheep that were left untreated and/or those carrying resistant nematodes. Specifically, the potentially beneficial genus Bifidobacterium was identified as elevated in successfully treated animals. The detection of these and other biomarkers will provide the basis for new therapeutic reagents particularly relevant to the problems of emerging multidrug anthelmintic resistance.

Effect of insulin in conjunction with glucose, amino acids and potassium on net metabolism of glucose and amino acids in the goat mammary gland

1992 ◽  
Vol 59 (2) ◽  
pp. 135-149 ◽  
Author(s):  
Sophie Tesseraud ◽  
Jean Grizard ◽  
Boguslaw Makarski ◽  
Elisabeth Debras ◽  
Gerard Bayle ◽  
...  
Keyword(s):  
Amino Acids ◽  
Mammary Gland ◽  
Insulin Infusion ◽  
Insulin Treatment ◽  
Essential Amino Acids ◽  
Dairy Goats ◽  
Experimental Conditions ◽  
Physiological Level ◽  
Acid Infusion ◽  
Amino Acid Infusion

SummaryThe hyperinsulinaemic euglycaemic insulin clamp technique was used to study the effect of insulin on the arterio-venous concentration differences of glucose and amino acids across the mammary gland in dairy goats. Insulin was given in conjunction with K to prevent insulin hypokalaemia. Appropriate amino acid infusion was used to blunt insulin-induced hypoaminoacidaemia or to create hyperaminoacidaemia and maintain this state under insulin treatment. Hyper-aminoacidaemia alone only stimulated mammary leucine uptake but did not significantly modify the net metabolism of other amino acids and glucose. Insulin infusion at physiological level in conjunction with glucose, KCl-NaCl and amino acids failed to alter mammary uptake of glucose and essential amino acids; occasional increase in arginine extraction and decrease in tyrosine extraction were exceptions. Thus these new experimental conditions did not reveal any galactopoietic effect of insulin.

Sign in / Sign up

Export Citation Format

Share Document