scholarly journals Transcriptome profiling of anthocyanin biosynthesis in the peel of ‘Granny Smith’ apples (Malus domestica) after bag removal

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Changqing Ma ◽  
Bowen Liang ◽  
Bo Chang ◽  
Jiuying Yan ◽  
Li Liu ◽  
...  
Keyword(s):  
Malus Domestica ◽  
Anthocyanin Biosynthesis ◽  
Transcriptome Profiling

scholarly journals Transcriptomic Analysis of the Anthocyanin Biosynthetic Pathway Reveals the Molecular Mechanism Associated with Purple Color Formation in Dendrobium Nestor

Life ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 113
Author(s):  
Xueqiang Cui ◽  
Jieling Deng ◽  
Changyan Huang ◽  
Xuan Tang ◽  
Xianmin Li ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Developmental Stages ◽  
Anthocyanin Biosynthesis ◽  
Transcriptome Profiling ◽  
Flower Color ◽  
Flower Bud ◽  
Color Formation ◽  
Color Development ◽  
Purple Coloration ◽  
Purple Color

Dendrobium nestor is a famous orchid species in the Orchidaceae family. There is a diversity of flower colorations in the Dendrobium species, but knowledge of the genes involved and molecular mechanism underlying the flower color formation in D. nestor is less studied. Therefore, we performed transcriptome profiling using Illumina sequencing to facilitate thorough studies of the purple color formation in petal samples collected at three developmental stages, namely—flower bud stage (F), half bloom stage (H), and full bloom stage (B) in D. nestor. In addition, we identified key genes and their biosynthetic pathways as well as the transcription factors (TFs) associated with purple flower color formation. We found that the phenylpropanoid–flavonoid–anthocyanin biosynthesis genes such as phenylalanine ammonia lyase, chalcone synthase, anthocyanidin synthase, and UDP-flavonoid glucosyl transferase, were largely up-regulated in the H and B samples as compared to the F samples. This upregulation might partly account for the accumulation of anthocyanins, which confer the purple coloration in these samples. We further identified several differentially expressed genes related to phytohormones such as auxin, ethylene, cytokinins, salicylic acid, brassinosteroid, and abscisic acid, as well as TFs such as MYB and bHLH, which might play important roles in color formation in D. nestor flower. Sturdy upregulation of anthocyanin biosynthetic structural genes might be a potential regulatory mechanism in purple color formation in D. nestor flowers. Several TFs were predicted to regulate the anthocyanin genes through a K-mean clustering analysis. Our study provides valuable resource for future studies to expand our understanding of flower color development mechanisms in D. nestor.

scholarly journals Fruit Coloration and Anthocyanin Biosynthesis after Bag Removal in Non-Red and Red Apples (Malus × domestica Borkh.)

Molecules ◽  
2013 ◽  
Vol 18 (2) ◽  
pp. 1549-1563 ◽  
Author(s):  
Yulian Liu ◽  
Fei Che ◽  
Lixin Wang ◽  
Rui Meng ◽  
Xiaojun Zhang ◽  
...  
Keyword(s):  
Malus Domestica ◽  
Anthocyanin Biosynthesis ◽  
Malus Domestica Borkh

Metabolomic and Transcriptomic Analysis of the Anthocyanin Regulatory Networks in Malus Domestica Borkh. Peel with Different Color Patterns

2021 ◽  
Author(s):  
Pengwei Duan ◽  
Xiaojian Ma ◽  
Lizhe Qin ◽  
Jizhuang Du ◽  
Guoliang Xu ◽  
...  
Keyword(s):  
Malus Domestica ◽  
Regulatory Networks ◽  
Anthocyanin Biosynthesis ◽  
Differentially Expressed ◽  
Color Patterns ◽  
Bhlh Genes ◽  
Apple Peel ◽  
Fuji Apple ◽  
Different Color ◽  
Apple Peels

Abstract Background:Coloring is an important external quality of ‘Fuji’ apple (Malus domestica Borkh.) and there are two color patterns of apple peels, i.e., stripe and blush. The objectives of this study were to reveal the anthocyanin biosynthesis metabolic pathway in striped and blushed peels of Malus domestica using metabolomics and transcriptomics, to identify different anthocyanin metabolites, and to analyze the differentially expressed genes involved in anthocyanin biosynthesis.Result:The metabolite concentration and gene expression were profiled in the striped and blushed fruit peels of apple harvested at three ripening periods to elucidate the color formation mechanism. At the green fruit period, there were 83 DAMs,including 30 flavonoids, 674 DEGs (521 up-regulated and 153 down-regulated),including 3 MYB related genes (up-regulated, LOC103415449, LOC103421948, LOC103432338) and 2 bHLH genes(up-regulated, LOC103436250, LOC103437863) between striped and blushed apple.At the color turning period, there were 48 DAMs,including 20 flavonoids, 880 DEGs (274 up-regulated and 606 down-regulated), including 3 differentially expressed E2.3.1.133, HCT genes(down-regulated), 2 differentially expressed F3H genes (down-regulated), 1 differentially expressed BZ1 gene (down-regulated) and 2 differentially expressed ANS genes (up-regulated) and 2 up-regulated MYB related genes (LOC103411576, LOC103412495), 5 down-regulated MYB related genes(LOC103400953, LOC103408672, LOC103415404, LOC103420697, LOC103421948), 1 differentially expressed bHLH gene(down-regulated, LOC103400870). At the complete coloring period，there were 95 DAMs,including 34 flavonoids, 2258 DEGs (1159 up- and 1099 down-regulated), including 3 differentially expressed E2.3.1.133, HCT genes(down-regulated), 1 differentially expressed E2.3.1.133, HCT genes(up-regulated), 2 differentially expressed CYP98A genes (up-regulated), 4 differentially expressed CHS genes (up-regulated), 2 differentially expressed E5.5.1.6 genes(up-regulated), 2 differentially expressed CYP75B1 genes (up-regulated), 2 differentially expressed F3R genes (up-regulated), 2 differentially expressed ANS genes (up-regulated), 1 differentially expressed DFR genes (up-regulated), 2 differentially expressed BZ1 genes (up-regulated) and 1 differentially expressed MYB related gene (up-regulated, LOC103401575) .There were both 10 kinds of cyanidin in apple peel at color turning period and complete coloring period, Keracyanin and Cyanin were up-regulated at color turning period and Cyanidin-3-O-(6''-O-malonyl)glucoside was up-regulated at complete coloring period.Conclusions: Our researches provide important information on the anthocyanin metabolites and the candidate genes involved in the anthocyanin biosynthesis pathways of Fuji apple in M.domestcia.

scholarly journals Transcriptome Profiling of Light-Regulated Anthocyanin Biosynthesis in the Pericarp of Litchi

2016 ◽  
Vol 07 ◽  
Author(s):  
Hong-Na Zhang ◽  
Wei-Cai Li ◽  
Hui-Cong Wang ◽  
Sheng-You Shi ◽  
Bo Shu ◽  
...  
Keyword(s):  
Anthocyanin Biosynthesis ◽  
Transcriptome Profiling
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