scholarly journals Independent modulation of individual genomic component transcription and a cis-acting element related to high transcriptional activity in a multipartite DNA virus

BMC Genomics ◽  
2019 ◽  
Vol 20 (1) ◽  
Author(s):  
Nai-Tong Yu ◽  
Hui-Min Xie ◽  
Yu-Liang Zhang ◽  
Jian-Hua Wang ◽  
Zhongguo Xiong ◽  
...  
Keyword(s):  
Transcriptional Activity ◽  
Genomic Component ◽  
Dna Virus ◽  
Cis Acting ◽  
High Transcriptional Activity

scholarly journals A combination of closely associated positive and negative cis-acting promoter elements regulates transcription of the skeletal alpha-actin gene.

1990 ◽  
Vol 10 (2) ◽  
pp. 528-538 ◽  
Author(s):  
K L Chow ◽  
R J Schwartz
Keyword(s):  
Transcriptional Activity ◽  
Primary Cultures ◽  
Gene Promoter ◽  
Regulatory Sequence ◽  
Actin Gene ◽  
Cell Type ◽  
Specific Expression ◽  
Cis Acting ◽  
Cell Type Specific Expression ◽  
Alpha Actin

The chicken skeletal alpha-actin gene promoter region provides at least a 75-fold-greater transcriptional activity in muscle cells than in fibroblasts. The cis-acting sequences required for cell type-restricted expression within this 200-base-pair (bp) region were elucidated by chloramphenicol acetyltransferase assays of site-directed Bg/II linker-scanning mutations transiently transfected into primary cultures. Four positive cis-acting elements were identified and are required for efficient transcriptional activity in myogenic cells. These elements, conserved across vertebrate evolution, include the ATAAAA box (-24 bp), paired CCAAT-box-associated repeats (CBARs; at -83 bp and -127 bp), and the upstream T+A-rich regulatory sequence (at -176 bp). Basal transcriptional activity in fibroblasts was not as dependent on the upstream CBAR or regions of the upstream T+A-rich regulatory sequence. Transfection experiments provided evidence that positive regulatory factors required for alpha-actin expression in fibroblasts are limiting. In addition, negative cis-acting elements were detected and found closely associated with the G+C-rich sequences that surround the paired CBARs. Negative elements may have a role in restricting developmentally timed expression in myoblasts and appear to inhibit promoter activity in nonmyogenic cells. Cell type-specific expression of the skeletal alpha-actin gene promoter is regulated by combinatorial and possibly competitive interactions between multiple positive and negative cis-acting elements.

scholarly journals High Transcriptional Activity and Diverse Functional Repertoires of Hundreds of Giant Viruses in a Coastal Marine System

mSystems ◽  
2021 ◽  
Author(s):  
Anh D. Ha ◽  
Mohammad Moniruzzaman ◽  
Frank O. Aylward
Keyword(s):  
Transcriptional Activity ◽  
Infectious Agents ◽  
Metabolic Processes ◽  
High Transcriptional Activity ◽  
Coastal Marine ◽  
Marine System ◽  
Giant Viruses

The discovery of giant viruses has transformed our understanding of viral complexity. Although viruses have traditionally been viewed as filterable infectious agents that lack metabolism, giant viruses can reach sizes rivalling cellular lineages and possess genomes encoding central metabolic processes.

Identification of a cis-acting element controlling induction of early gene expression in Dictyostelium discoideum

1989 ◽  
Vol 9 (11) ◽  
pp. 4653-4659
Author(s):  
T May ◽  
H Kern ◽  
A Müller-Taubenberger ◽  
W Nellen
Keyword(s):  
Dictyostelium Discoideum ◽  
Transcriptional Activity ◽  
Developmental Regulation ◽  
Protein A ◽  
Early Gene ◽  
Related Protein ◽  
Developmentally Regulated ◽  
Cis Acting ◽  
Transformation Vector ◽  
Low Levels

Using a new promoter analysis transformation vector for Dictyostelium discoideum (PAV-CAT), we have defined cis-acting elements in the promoter of the cyclic AMP-induced early-expressed gene A11H2, which encodes an alpha-fucosidase-related protein (A. Müller-Taubenberger, M. Westphal, A. Noegel, and G. Gerisch, FEBS Lett. 246:185-192, 1989). Sequences responsible for developmentally regulated gene induction could be separated from the basal promoter that conferred low levels of transcriptional activity. By gel shift experiments, we present evidence that the cis-acting element is the target of a trans-acting factor that by itself is subject to developmental regulation.

scholarly journals A human tRNAGlugene of high transcriptional activity

1983 ◽  
Vol 11 (9) ◽  
pp. 2551-2562 ◽  
Author(s):  
J.P. Goddard ◽  
M. Squire ◽  
M Bienz ◽  
J.D. Smith
Keyword(s):  
Transcriptional Activity ◽  
High Transcriptional Activity

scholarly journals Identification of a cis-acting element controlling induction of early gene expression in Dictyostelium discoideum.

1989 ◽  
Vol 9 (11) ◽  
pp. 4653-4659 ◽  
Author(s):  
T May ◽  
H Kern ◽  
A Müller-Taubenberger ◽  
W Nellen
Keyword(s):  
Dictyostelium Discoideum ◽  
Transcriptional Activity ◽  
Developmental Regulation ◽  
Protein A ◽  
Early Gene ◽  
Related Protein ◽  
Developmentally Regulated ◽  
Cis Acting ◽  
Transformation Vector ◽  
Low Levels

Using a new promoter analysis transformation vector for Dictyostelium discoideum (PAV-CAT), we have defined cis-acting elements in the promoter of the cyclic AMP-induced early-expressed gene A11H2, which encodes an alpha-fucosidase-related protein (A. Müller-Taubenberger, M. Westphal, A. Noegel, and G. Gerisch, FEBS Lett. 246:185-192, 1989). Sequences responsible for developmentally regulated gene induction could be separated from the basal promoter that conferred low levels of transcriptional activity. By gel shift experiments, we present evidence that the cis-acting element is the target of a trans-acting factor that by itself is subject to developmental regulation.

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