The barley stripe mosaic virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

Abstract Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization.Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected.Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

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The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

10.21203/rs.2.16980/v2 ◽

2020 ◽

Author(s):

Arnaud Cheuk ◽

François Ouellet ◽

Mario Houde

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Mosaic Virus ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Expression System ◽

Functional Characterization ◽

Barley Stripe Mosaic Virus ◽

Finger Protein

Abstract Background Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization. Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected. Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

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The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

10.21203/rs.2.16980/v3 ◽

2020 ◽

Author(s):

Arnaud Cheuk ◽

François Ouellet ◽

Mario Houde

Keyword(s):

Drought Stress ◽

Drought Tolerance ◽

Mosaic Virus ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Expression System ◽

Functional Characterization ◽

Barley Stripe Mosaic Virus ◽

Finger Protein

Abstract Background Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization. Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected. Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

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Transcriptional Repression Mediated by the KRAB Domain of the Human C2H2 Zinc Finger Protein Kox1/ZNF10 Does Not Require Histone Deacetylation

Biological Chemistry ◽

10.1515/bc.2001.075 ◽

2001 ◽

Vol 382 (4) ◽

Cited By ~ 20

Author(s):

Peter Lorenz ◽

Dirk Koczan ◽

Hans-Jürgen Thiesen

Keyword(s):

Zinc Finger ◽

Transcriptional Repression ◽

Zinc Finger Protein ◽

Histone Deacetylation ◽

C2h2 Zinc Finger ◽

Finger Protein ◽

Krab Domain ◽

C2h2 Zinc Finger Protein

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A novel C2H2 zinc-finger protein gene (ZNF160) maps to human chromosome 19q13.3–q13.4

Genomics ◽

10.1016/0888-7543(95)80149-g ◽

1995 ◽

Vol 25 (1) ◽

pp. 322-323 ◽

Cited By ~ 4

Author(s):

Stephanie Halford ◽

Marie-Genevieve Mattei ◽

Sara Daw ◽

Peter J. Scambler

Keyword(s):

Human Chromosome ◽

Zinc Finger ◽

Protein Gene ◽

Zinc Finger Protein ◽

C2h2 Zinc Finger ◽

Zinc Finger Protein Gene ◽

Finger Protein ◽

C2h2 Zinc Finger Protein

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Abstract 2392: Transcriptional repression of VEGF by Znf24, a C2H2 zinc finger protein

10.1158/1538-7445.am10-2392 ◽

2010 ◽

Author(s):

Di Jia ◽

Robyn Loureiro ◽

Patricia D'Amore ◽

Scott Rodig ◽

Marsha Moses

Keyword(s):

Zinc Finger ◽

Transcriptional Repression ◽

Zinc Finger Protein ◽

C2h2 Zinc Finger ◽

Finger Protein ◽

C2h2 Zinc Finger Protein

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ZNF300, a recently identified human transcription factor, activates the human IL-2Rβ promoter through the overlapping ZNF300/EGR1 binding site

Cellular & Molecular Biology Letters ◽

10.2478/s11658-010-0025-1 ◽

2010 ◽

Vol 15 (4) ◽

Cited By ~ 3

Author(s):

Lu Xue ◽

Hongling Qiu ◽

Jian Ma ◽

Mingxiong Guo ◽

Wenxin Li

Keyword(s):

Binding Site ◽

Zinc Finger ◽

Zinc Finger Protein ◽

Protein Family ◽

Binding Property ◽

Mobility Shift ◽

Transient Transfection Assay ◽

C2h2 Zinc Finger ◽

Finger Protein ◽

C2h2 Zinc Finger Protein

AbstractZNF300 was recently identified as a member of the human KRAB/C2H2 zinc finger protein family. Little is known about the role of ZNF300 in human gene regulation networks. In this study, the DNA-binding property of ZNF300 was further analyzed. We found that the recombinant ZNF300 could bind to the binding site 5′-GCGGGGGCG-3′ of Egr1, another member of the KRAB/C2H2 zinc finger protein family. Similarly, recombinant Egr1 also showed a similar binding affinity to the ZNF300 binding site 5′-CTGGGGGCG-3′. Bioinformatics analysis revealed that there is an overlapping ZNF300/Egr1 binding site in the human IL-2Rβ promoter region, which was previously known to be recognized by endogenous Egr1. Electrophoretic mobility shift assays showed that endogenous ZNF300 could also bind to this site. A transient transfection assay revealed that both ZNF300 and Egr1 could transactivate the IL-2Rβ promoter, and that the activation was abrogated by a mutation of residues in the overlapping ZNF300/Egr1 binding site. Co-expression of ZNF300 and Egr1 led to enhanced IL-2Rβ promoter activity. Thus, ZNF300 is likely to be another regulator of the human IL-2Rβ promoter.

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