Tubularized urethral reconstruction using everted saphenous vein graft in a beagle model

Abstract Background A long segment stricture in the anterior urethra is a challenge in urology. We conducted a study to investigate the efficacy of anterior urethral reconstruction using an everted saphenous vein graft (SVG) in a tubular fashion. Methods Twelve male beagles were randomly divided into three groups: experimental group (n = 5), control group (n = 5) and normal group (n = 2). A 3 cm defect in the anterior urethra was created. Autologous SVG was harvested. In the experimental group, urethral defect was replaced by an everted SVG in a tubular fashion. In the control group, urethral reconstruction was performed using an uneverted SVG. Beagles in all groups received retrograde urethrography to evaluate urethral patency and were killed for histological examination 6 months after operation. Results Four beagles in the experimental group had no voiding difficulty and the other one could not void spontaneously. Retrograde urethrography showed the four beagles in experimental group had wide urethral lumens. Ether urethral stricture or fistula were detected in all animals in the control group. Histological analysis of the four beagles in the experimental group indicated the everted SVG completely integrated into the urethra. The reconstructed urethra contained a wide lumen and was completely covered by urothelium. The periurethral collagen and muscle fibers formed and were highly organized. Everted SVG showed a high ability of neovascularization. In the control group, the reconstructed segment showed a fibrotic urethral lumen where the urothelium was not intact. Only few new capillaries were formed. Conclusions Everted SVG demonstrates for a promising strategy for potential urethral stricture repair.

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Tubularized Urethral Reconstruction Using Everted Saphenous Vein Graft in A Beagle Model

10.21203/rs.3.rs-74278/v1 ◽

2020 ◽

Author(s):

Dan Li ◽

Zhou Shen ◽

Yujie Xu

Keyword(s):

Urethral Stricture ◽

Saphenous Vein ◽

Vein Graft ◽

Saphenous Vein Graft ◽

Control Group ◽

Urethral Reconstruction ◽

Urethral Strictures ◽

Retrograde Urethrography ◽

Urethral Defect ◽

Experimental Group

Abstract Background: The repair of anterior urethral stricture and hypospadias require urethral reconstruction. We conduct the study to investigate the efficacy of anterior urethral reconstruction using everted saphenous vein graft in tubular fashion. Methods: Twelve male beagles were randomly divided into three groups: experimental group( n=5), control group( n=5) and normal group( n=2). A 3 cm defect in anterior urethra was created. Autologous saphenous vein graft was harvested. In experimental group, urethral defect was replaced by everted saphenous vein graft in tubular fashion. In control group, urethral reconstruction was performed using uneverted saphenous vein graft. At 6 months postoperatively, beagles in all groups received retrograde urethrography to evaluate urethral patency and were killed for histological examination. Results: Retrograde urethrography showed the urethral caliber of beagles in experimental group were similar to those of normal. Ether urethral stricture or fistula were detected in all animals in control group. In experimental group, the everted saphenous vein graft completely integrated into the urethra. The reconstructed urethra had a wide lumen and was completely covered by urothelium. The subepithelial collagen and muscle fibers were well formed and highly organized. Everted saphenous vein graft had a high ability of neovascularization. In control group, the reconstructed segment had a fibrotic urethral lumen in which urothelium was not intact. And there were few newly formed capillaries. Conclusions: Everted saphenous vein graft can be used as tubular substitute material for anterior urethral strictures and hypospadias.

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Urethral Reconstruction Using Everted Saphenous Vein Graft in a Rabbit Model: One-Year Outcomes

Urologia Internationalis ◽

10.1159/000464473 ◽

2017 ◽

Vol 99 (1) ◽

pp. 110-117 ◽

Cited By ~ 1

Author(s):

Yujie Xu ◽

Zhou Shen ◽

Gang Liu ◽

Bing Liu ◽

Xiaoliang Hua ◽

...

Keyword(s):

Saphenous Vein ◽

Vein Graft ◽

Saphenous Vein Graft ◽

Rabbit Model ◽

Urethral Reconstruction ◽

One Year

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Effect of Abciximab on cardiac enzyme elevation after transluminal extraction atherectomy (TEC) in high-risk saphenous vein graft lesions: Comparison with a historical control group

Catheterization and Cardiovascular Interventions ◽

10.1002/1522-726x(200101)52:1<40::aid-ccd1010>3.0.co;2-f ◽

2001 ◽

Vol 52 (1) ◽

pp. 40-44 ◽

Cited By ~ 5

Author(s):

Masroor A. Khan ◽

Ming W. Liu ◽

Francisco L. Chio ◽

Virginia B. Yates ◽

Gregory D. Chapman ◽

...

Keyword(s):

High Risk ◽

Saphenous Vein ◽

Vein Graft ◽

Saphenous Vein Graft ◽

Historical Control Group ◽

Historical Control ◽

Control Group ◽

Cardiac Enzyme ◽

Enzyme Elevation

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Cell-Seeded Acellular Artery for Reconstruction of Long Urethral Defects in a Canine Model

Stem Cells International ◽

10.1155/2021/8854479 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Hao Zhong ◽

Yamei Shen ◽

Danhui Zhao ◽

Guoqiang Yan ◽

Chengai Wu ◽

...

Keyword(s):

Stem Cells ◽

Urethral Stricture ◽

Canine Model ◽

Progressive Increase ◽

Control Group ◽

Adipose Derived Stem Cells ◽

Tissue Formation ◽

Urethral Defect ◽

Experimental Group ◽

Over Time

The management of urethral stricture remains a major therapeutic challenge in clinics. Herein, we explored the feasibility of reconstructing a relatively long segment of the urethra by the cell-seeded acellular artery in a canine model. The acellular arterial matrix was obtained from the excised carotid artery of donor dogs. Autologous adipose-derived stem cells (ADSCs) from 6 male dogs were grown and seeded onto the premade acellular arterial matrix. A 3 cm long segment of the urethra was resected in 12 male dogs. Urethroplasty was performed with the acellular arterial matrix seeded with ADSCs in 6 animals and without cells in 6. Serial urethrography was performed at 1 and 3 months postoperatively. Wide urethral calibers without any signs of strictures were confirmed in all 6 animals in the experimental group. In contrast, urethral stricture was demonstrated in 3 animals in the control group. The graft was highly epithelialized and smooth in the experimental group, while graft contracture and scar formation were showed in the control group. Histologic analysis of the cell-seeded arterial matrix at 1 month confirmed the presence of multilayered urothelium and muscle. The levels of tissue formation developed over time with a progressive increase in muscle content. In contrast, extensive fibrosis and sparse smooth muscle were seen in animals treated with matrix without ADSCs. This study provides preclinical evidence that the ADSC-seeded arterial matrix can be used as a tubularized scaffold in the reconstruction of 3 cm long urethral defect in a male canine model. The ADSC-seeded arterial matrix remodels and regenerates normal-appearing urethral tissue layers over time.

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