Bacterial diversity and functional analysis of severe early childhood caries and recurrence in India

AbstractDental caries is the most prevalent oral disease affecting nearly 70% of children in India and elsewhere. Micro-ecological niche based acidification due to dysbiosis in oral microbiome are crucial for caries onset and progression. Here we report the tooth bacteriome diversity compared in Indian children with caries free (CF), severe early childhood caries (SC) and recurrent caries (RC). High quality V3–V4 amplicon sequencing revealed that SC exhibited high bacterial diversity with unique combination and interrelationship. Gracillibacteria_GN02 and TM7 were unique in CF and SC respectively, while Bacteroidetes, Fusobacteria were significantly high in RC. Interestingly, we found Streptococcus oralis subsp. tigurinus clade 071 in all groups with significant abundance in SC and RC. Positive correlation between low and high abundant bacteria as well as with TCS, PTS and ABC transporters were seen from co-occurrence network analysis. This could lead to persistence of SC niche resulting in RC. Comparative in vitro assessment of biofilm formation showed that the standard culture of S. oralis and its phylogenetically similar clinical isolates showed profound biofilm formation and augmented the growth and enhanced biofilm formation in S. mutans in both dual and multispecies cultures.

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Characterization of Supragingival Plaque and Oral Swab Microbiomes in Children With Severe Early Childhood Caries

Frontiers in Microbiology ◽

10.3389/fmicb.2021.683685 ◽

2021 ◽

Vol 12 ◽

Author(s):

Vivianne Cruz de Jesus ◽

Mohd Wasif Khan ◽

Betty-Anne Mittermuller ◽

Kangmin Duan ◽

Pingzhao Hu ◽

...

Keyword(s):

Early Childhood ◽

Dental Plaque ◽

Early Childhood Caries ◽

Oral Microbiome ◽

Rrna Genes ◽

Oral Swab ◽

Supragingival Plaque ◽

Severe Early Childhood Caries ◽

Childhood Caries

The human oral cavity harbors one of the most diverse microbial communities with different oral microenvironments allowing the colonization of unique microbial species. This study aimed to determine which of two commonly used sampling sites (dental plaque vs. oral swab) would provide a better prediction model for caries-free vs. severe early childhood caries (S-ECC) using next generation sequencing and machine learning (ML). In this cross-sectional study, a total of 80 children (40 S-ECC and 40 caries-free) < 72 months of age were recruited. Supragingival plaque and oral swab samples were used for the amplicon sequencing of the V4-16S rRNA and ITS1 rRNA genes. The results showed significant differences in alpha and beta diversity between dental plaque and oral swab bacterial and fungal microbiomes. Differential abundance analyses showed that, among others, the cariogenic species Streptococcus mutans was enriched in the dental plaque, compared to oral swabs, of children with S-ECC. The fungal species Candida dubliniensis and C. tropicalis were more abundant in the oral swab samples of children with S-ECC compared to caries-free controls. They were also among the top 20 most important features for the classification of S-ECC vs. caries-free in oral swabs and for the classification of dental plaque vs. oral swab in the S-ECC group. ML approaches revealed the possibility of classifying samples according to both caries status and sampling sites. The tested site of sample collection did not change the predictability of the disease. However, the species considered to be important for the classification of disease in each sampling site were slightly different. Being able to determine the origin of the samples could be very useful during the design of oral microbiome studies. This study provides important insights into the differences between the dental plaque and oral swab bacteriome and mycobiome of children with S-ECC and those caries-free.

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Genetic polymorphism of scrA gene of Streptococcus mutans isolates is not associated with biofilm formation in severe early childhood caries

BMC Oral Health ◽

10.1186/s12903-017-0407-0 ◽

2017 ◽

Vol 17 (1) ◽

Cited By ~ 2

Author(s):

Yan Zhou ◽

Lixia Yu ◽

Ye Tao ◽

Qinghui Zhi ◽

Huancai Lin

Keyword(s):

Early Childhood ◽

Genetic Polymorphism ◽

Streptococcus Mutans ◽

Biofilm Formation ◽

Early Childhood Caries ◽

Severe Early Childhood Caries ◽

Childhood Caries

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An in-vitro evaluation of antibacterial effect of Amalgomer CR and Fuji VII against bacteria causing severe early childhood caries

Journal of Indian Society of Pedodontics and Preventive Dentistry ◽

10.4103/0970-4388.175506 ◽

2016 ◽

Vol 34 (1) ◽

pp. 23 ◽

Cited By ~ 2

Author(s):

PraveenkumarS Mandroli ◽

RajeshHemant Bariker

Keyword(s):

Early Childhood ◽

Early Childhood Caries ◽

Antibacterial Effect ◽

In Vitro Evaluation ◽

Severe Early Childhood Caries ◽

Childhood Caries

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Magnetic Bead-Based Salivary Peptidome Profiling Analysis for Severe Early Childhood Caries

Caries Research ◽

10.1159/000360868 ◽

2014 ◽

Vol 49 (1) ◽

pp. 63-69 ◽

Cited By ~ 8

Author(s):

Yan Si ◽

Shuang Ao ◽

Weijian Wang ◽

Feng Chen ◽

Shuguo Zheng

Keyword(s):

Mass Spectrometry ◽

Early Childhood ◽

Early Childhood Caries ◽

Expression Profiles ◽

Salivary Protein ◽

Preschool Age ◽

Diagnostic Model ◽

Peptide Mass ◽

Severe Early Childhood Caries ◽

Childhood Caries

Objective: To investigate the differential salivary protein expression profiles between children with severe early childhood caries (S-ECC) and caries-free (CF) children at the age of 3 years. Methods: We used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) combined with weak cation exchange magnetic beads, and peptide mass fingerprints were created by scanning mass spectrometry signals. Salivary samples from 20 children were analyzed (10 for each group). Results: Eleven protein peaks were significantly different (p < 0.05) between the two groups. Eight of these peaks were higher in the S-ECC group and three were higher in the CF group. To establish a diagnostic model for discrimination between the two groups, we chose three peptides (3,186.2, 3,195.8 and 3,324.8 Da) that exhibited the best fitted curve, by which the two groups were better separated when compared with other combinations. Conclusions: The salivary biomarkers identified revealed significant differences between the CF and the S-ECC group. Our results provide novel insight into the salivary protein profile of preschool-age children with dental caries and may lead to the development of a new strategy for screening high-risk populations.

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Dental Treatments under the General Anesthesia in a Child with Keratitis, Ichthyosis, and Deafness Syndrome

Case Reports in Dentistry ◽

10.1155/2013/618468 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6

Author(s):

Sera Sımsek Derelioglu ◽

Yücel Yılmaz ◽

Sultan Keles

Keyword(s):

Early Childhood ◽

Case Report ◽

General Anesthesia ◽

Early Childhood Caries ◽

Sensorineural Deafness ◽

Oral Symptoms ◽

Severe Early Childhood Caries ◽

Childhood Caries

KID syndrome is a rare genodermatosis characterized by keratitis, ichthyosis, and sensorineural deafness. Although the dermatological, ophthalmologic, and sensorineural defects are emphasized in the literature, oral and dental evaluations are so superficial. In this case report, dental and oral symptoms of a three year and five months old boy with KID syndrome, suffering severe Early Childhood Caries (s-ECC) and dental treatments done under General Anesthesia (GA) were reported.

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