scholarly journals Expression of early angiogenesis indicators in mature versus immature teeth

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Javier Caviedes-Bucheli ◽  
Luis F. Lopez-Moncayo ◽  
Hernan Dario Muñoz-Alvear ◽  
Francisco Hernandez-Acosta ◽  
Melissa Pantoja-Mora ◽  
...  
Keyword(s):  
Root Development ◽  
Dental Pulp ◽  
Cell Metabolism ◽  
Elisa Test ◽  
Physiological Process ◽  
Oxygen Balance ◽  
Third Molars ◽  
Angiogenic Activity ◽  
Immature Teeth ◽  
Development Group

Abstract Background Proper oxygen balance in the dental pulp is essential for cell metabolism. Angiogenesis in the pulp is a constant process during the life of the tooth. Hypoxia indicators in a tissue, such as HIF-1α, as well as vascular destabilization markers, such as ANG2 and its receptor TIE2, are necessary for angiogenesis. Therefore the purpose of this study is to evaluate the expression of HIF-1α, ANG1, ANG2 and TIE2 in dental pulp as early angiogenesis indicators in teeth with complete and incomplete root development. Methods Forty human dental pulps were obtained from freshly extracted third molars divided into two groups: incomplete (n = 20) and complete (n = 20) root development. Dental pulps were stored at − 80 °C, defrosted in an ice bath and re-frozen with their respective thaws to disintegrate the tissue. Three sonication cycles were performed until the tissues were homogenized, then thaw were centrifuged and the supernatant was collected for the detection of the markers to be studied. The samples were processed for the ELISA test using the ELISA-sandwich principle. Student t and Mann–Whitney U tests were performed to determine statistically significant differences between groups. Results In the complete root development, HIF-1α, ANG1, ANG2 and TIE2 expressions were significantly higher than their expression in the incomplete root development group. Conclusions The angiogenic process seems to be a physiological process in the dental pulp. Angiogenic activity is higher in teeth with mature than immature apex teeth.

Vital Pulp Therapy Current Trends and Future Perspectives

2021 ◽  
Author(s):  
Iris Slutzky-Goldberg
Keyword(s):  
Root Development ◽  
Future Perspectives ◽  
Direct Pulp Capping ◽  
Pulp Capping ◽  
Pulp Therapy ◽  
Immature Teeth ◽  
Current Trends ◽  
Vital Pulp Therapy ◽  
Irreversible Pulpitis

Vital pulp therapy (VPT), including direct pulp capping, partial and cervical Pulpotomy, was suggested for the treatment of young teeth, with reversible or irreversible pulpitis [1]. Maintaining the vitality of immature teeth enables continued root development, maturogenesis, and a better prognosis

Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet-rich plasma and dental pulp cells

2013 ◽  
Vol 46 (10) ◽  
pp. 962-970 ◽  
Author(s):  
W. Zhu ◽  
X. Zhu ◽  
G. T.-J. Huang ◽  
G. S. P. Cheung ◽  
W. L. Dissanayaka ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Platelet Rich Plasma ◽  
Apical Periodontitis ◽  
Dental Pulp Cells ◽  
Pulp Tissue ◽  
Immature Teeth ◽  
Dental Pulp Tissue ◽  
Pulp Cells

scholarly journals Manufacturing of dental pulp cell-based products from human third molars: current strategies and future investigations

2015 ◽  
Vol 6 ◽  
Author(s):  
Maxime Ducret ◽  
Hugo Fabre ◽  
Olivier Degoul ◽  
Gianluigi Atzeni ◽  
Colin McGuckin ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Third Molars ◽  
Pulp Cell ◽  
Dental Pulp Cell

scholarly journals Characterization of Dental Pulp Stem Cells from Impacted Third Molars Cultured in Low Serum-Containing Medium

2011 ◽  
Vol 193 (6) ◽  
pp. 344-365 ◽  
Author(s):  
Jana Karbanová ◽  
Tomáš Soukup ◽  
Jakub Suchánek ◽  
Robert Pytlík ◽  
Denis Corbeil ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Third Molars ◽  
Low Serum

Enhanced regeneration potential of mobilized dental pulp stem cells from immature teeth

Oral Diseases ◽  
2017 ◽  
Vol 23 (5) ◽  
pp. 620-628 ◽  
Author(s):  
H Nakayama ◽  
K Iohara ◽  
Y Hayashi ◽  
Y Okuwa ◽  
K Kurita ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Regeneration Potential ◽  
Immature Teeth

scholarly journals Pulp Revascularisation of a Non-Vital Immature Young Permanent Tooth - A Case Report and Literature Review

2021 ◽  
Vol 10 (11) ◽  
pp. 845-848
Author(s):  
Tanvi Sanjay Satpute ◽  
Jayeeta Sidharth Verma ◽  
Jimish Rajiv Shah ◽  
Aditya Kiran Shinde
Keyword(s):  
Case Report ◽  
Root Development ◽  
Root Canal ◽  
Mineral Trioxide Aggregate ◽  
Signs And Symptoms ◽  
Permanent Tooth ◽  
Duration Of Treatment ◽  
Pulp Tissue ◽  
Dental Tissues ◽  
Immature Teeth

Traumatic injuries to an immature permanent tooth may result in cessation of dentin deposition and root maturation. Novel revascularisation endodontic procedure (REP) has been considered as an option for treatment of immature teeth with damaged pulp tissue. The continuous development of the root and the root canal has been recognised as a major advantage of this technique over traditional apexification approach. Traditional apexification procedures may resolve pathology but have not been able to prove tooth survival due to absence of continued root development and risk of root fracture. A successful REP results in resolution of signs and symptoms of pathology, radiographic signs of healing, proof of continued root development as well as presence of pulp vitality due to the regeneration of pulp tissue in the root canal. Currently, repair rather than true regeneration of the ‘pulp-dentine complex’ is achieved and further root maturation is variable. According to Glossary of Endodontic terms published by American Association of Endodontists, REP’s are biologically based procedures designed to physiologically replace damaged tooth structures, including dentin and root structures, as well as cells of the pulp-dentin complex.1,2 Apexification treatment has been a routine procedure to treat and preserve such teeth for many decades.3 Apexification is the process by which a suitable environment is created within the root canal and periapical tissue to allow for the formation of a calcific barrier across the open apex. Calcium hydroxide [Ca(OH)2] has been the material of choice for apexification as Frank reported its capacity to induce physiological closure of immature pulpless teeth in 1966.4 However, this technique has several disadvantages, including the unpredictability of apical barrier formation and the long duration of treatment, which often requires multiple visits.5 A retrospective study by Jeruphuaan et al.6 has shown a higher survival rate with regenerative endodontic treatment when compared to both mineral trioxide aggregate (MTA) and Ca(OH)2 apexification. The first evidence of regeneration of dental tissues was in 1932 by G.L. Feldman, who showed evidence of regeneration of dental pulp under certain optimal biological conditions.7 In 1971, a pioneer study in regenerative endodontics conducted by Nygaard-Ostby concluded that bleeding induced within a vital or necrotic canal led to resolution of signs and symptoms of necrotic cases and in certain cases, apical closure.8 According to Windley et al. (2005), the successful revascularisation of immature teeth with apical periodontitis is mainly dependent upon: 1. Canal disinfection 2. Scaffold placement in the canal for the growing tissues 3. Bacteria-tight sealing of the access opening.9 The purpose of this case report is to illustrate the outcome of a revascularisation endodontic procedure in a non-vital immature young permanent central incisor.

scholarly journals EFFECT OF TRIPLE ANTIBIOTIC PASTE, CALCIUM HYDROXIDE, LEDERMIX® ON VIABILITY OF PULP MESENCHYMAL STEM CELLS

2019 ◽  
pp. 49-53 ◽  
Author(s):  
EMIRIA DITA PRASANTI ◽  
ANGGRAINI MARGONO ◽  
NILAKESUMA DJAUHARIE
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Hydroxide ◽  
Dental Pulp ◽  
Primary Cultures ◽  
Immunofluorescence Assay ◽  
Control Group ◽  
Third Molars ◽  
Pulp Tissue ◽  
Triple Antibiotic Paste

Objective: The goal of regenerative endodontic therapy is biological healing of the pulp tissue. It involves the disinfection of the canals with irrigantsand medicaments. The medicaments that are currently used for this purpose are a triple antibiotic paste (TAP), calcium hydroxide (Ca[OH]2), andLedermix®, a paste containing demeclocycline and triamcinolone. Therefore, the purpose of this study was to evaluate the effects of TAP, Ca(OH)2, andLedermix® on the viability of dental pulp stem cells (DPSC).Methods: Primary cultures of DPSC were obtained from immature third molars. Immunofluorescence assay using STRO-1 marker was performedto confirm the mesenchymal nature of the DPSC. The cells were exposed to TAP, Ca(OH)2, and Ledermix® at concentrations of 0.1 and 1 mg/mL. Cellviability was analyzed using the MTT assay.Results: Significant differences in viability were noted between the cells exposed to the medicaments and those in the control group (p<0.05).Conclusions: All three medicaments decreased the viability of DPSC, with the Ledermix® paste demonstrating the highest toxic effect.

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