scholarly journals Plasmodium falciparum Kelch 13 mutations and treatment response in patients in Hpa-Pun District, Northern Kayin State, Myanmar

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Craig A. Bonnington ◽  
Aung Pyae Phyo ◽  
Elizabeth A. Ashley ◽  
Mallika Imwong ◽  
Kanlaya Sriprawat ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Treatment Response ◽  
Kelch 13

scholarly journals Increase in Kelch 13 Polymorphisms in Plasmodium falciparum, Southern Rwanda

2021 ◽  
Vol 27 (1) ◽  
pp. 294-296
Author(s):  
Clara Bergmann ◽  
Welmoed van Loon ◽  
Felix Habarugira ◽  
Costanza Tacoli ◽  
Julia C. Jäger ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Kelch 13

scholarly journals Expansion of the Plasmodium falciparum Kelch 13 R622I mutation in Northwest Ethiopia

2021 ◽  
Author(s):  
Aberham A. Alemayehu ◽  
Daniel Castaneda-Mogollon ◽  
Habtie Tesfa ◽  
Sisay Getie ◽  
Abu Naser Mohon ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Artemisinin Resistance ◽  
Northwest Ethiopia ◽  
Efficacy Trial ◽  
North West ◽  
North West Ethiopia ◽  
Parasitological Response ◽  
Kelch 13

Abstract According to the WHO, almost two thirds of the Ethiopian population are at risk of contracting malaria, where infection with Plasmodium falciparum accounts for approximately 60% of cases today. The risk of artemisinin resistance spreading from SE Asia to Africa is a major concern. We conducted a 28-day in vivo efficacy trial of Artemether-Lumefantrine (Co-Artem) for treatment of uncomplicated malaria (n = 97) in the Gondar Region, North West Ethiopia in 2017–2018. Our results confirmed 100% adequate clinical and parasitological response (ACPR) with no parasites observed at day 3 by microscopy. Further analysis of day 0 samples showed the expansion of a kelch13 mutation R622I to 9.5% from 2.4% of isolates reported three years earlier. Closer examination of the R622I mutation in vitro is warranted.

scholarly journals Efficacy of Artemether-Lumefantrine on various Plasmodium falciparum Kelch 13 and Pfmdr1 genes isolated in Ghana

2020 ◽  
Vol 11 ◽  
pp. e00190
Author(s):  
Enoch Aninagyei ◽  
Comfort Dede Tetteh ◽  
Martin Oppong ◽  
Alex Boye ◽  
Desmond Omane Acheampong
Keyword(s):  
Plasmodium Falciparum ◽  
Kelch 13

scholarly journals Polymorphisms in Plasmodium falciparum Kelch 13 and P. vivax Kelch 12 Genes in Parasites Collected from Three South Pacific Countries Prior to Extensive Exposure to Artemisinin Combination Therapies

2019 ◽  
Vol 63 (7) ◽  
Author(s):  
Karryn Gresty ◽  
Karen Anderson ◽  
Cielo Pasay ◽  
Norman C. Waters ◽  
Qin Cheng
Keyword(s):  
Plasmodium Falciparum ◽  
Papua New Guinea ◽  
Solomon Islands ◽  
Artemisinin Resistance ◽  
South Pacific ◽  
New Guinea ◽  
Combination Therapies ◽  
Baseline Information ◽  
Artemisinin Combination Therapies ◽  
Kelch 13

ABSTRACT The South Pacific countries Solomon Islands, Vanuatu, and Papua New Guinea (PNG) adopted artemisinin-based combination therapies (ACTs) in 2008. We examined Kelch 13 and Kelch 12 genes in parasites originating from these countries before or at ACT introduction. Four Kelch 13 and two Kelch 12 novel sequence polymorphisms, not associated with artemisinin resistance, were observed in parasites from Solomon Islands and Vanuatu. No polymorphisms were observed in PNG parasites. The findings provide useful baseline information.

scholarly journals Continued Sensitivity of Plasmodium falciparum to Artemisinin in Guyana, With Absence of Kelch Propeller Domain Mutant Alleles

2016 ◽  
Vol 3 (3) ◽  
Author(s):  
Reyaud Rahman ◽  
Maria Jesus Sanchez Martin ◽  
Shamdeo Persaud ◽  
Nicolas Ceron ◽  
Dwayne Kellman ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Confidence Interval ◽  
Clearance Rate ◽  
Artemisinin Resistance ◽  
Parasite Clearance ◽  
Wild Type ◽  
Resistance Monitoring ◽  
Mining Areas ◽  
Widespread Availability ◽  
Kelch 13

Abstract Because of concerns about possible emergence of artemisinin resistance strains of Plasmodium falciparum in mining areas of the interior of Guyana, a 7-day artesunate trial was conducted from March to December 2014. The day-3 parasite clearance rate, the efficacy of artesunate at day 28, and polymorphism of Kelch 13 (PfK13)—the marker of artemisinin resistance—were assessed. The study confirmed the continued sensitivity of P falciparum to artemisinin. A 7-day course of artesunate was 100% efficacious with only 2% (95% confidence interval, .1%–10.9%) of enrolled subjects positive at day 3. All day-0 parasite samples were wild type. Continued resistance monitoring is nevertheless recommended, given the widespread availability and uncontrolled use of artemisinin drugs in mining areas of Guyana.

Plasmodium falciparum kelch 13: a potential molecular marker for tackling artemisinin-resistant malaria parasites

2015 ◽  
Vol 14 (1) ◽  
pp. 125-135 ◽  
Author(s):  
Toshihiro Mita ◽  
Shin-Ichiro Tachibana ◽  
Muneaki Hashimoto ◽  
Makoto Hirai
Keyword(s):  
Plasmodium Falciparum ◽  
Molecular Marker ◽  
Malaria Parasites ◽  
Kelch 13

scholarly journals Kelch 13 propeller gene polymorphism among Plasmodium falciparum isolates in Lagos, Nigeria: Molecular Epidemiologic Study

2019 ◽  
Vol 24 (8) ◽  
pp. 1011-1017 ◽  
Author(s):  
Uche Igbasi ◽  
Wellington Oyibo ◽  
Sunday Omilabu ◽  
Hong Quan ◽  
Shen‐Bo Chen ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Gene Polymorphism ◽  
Epidemiologic Study ◽  
Molecular Epidemiologic Study ◽  
Kelch 13

scholarly journals Imidazolopiperazines Kill both Rings and Dormant Rings in Wild-Type and K13 Artemisinin-Resistant Plasmodium falciparum In Vitro

2018 ◽  
Vol 62 (5) ◽  
Author(s):  
Laurent Dembele ◽  
Devendra Kumar Gupta ◽  
Michelle Yi-Xiu Lim ◽  
Xiaoman Ang ◽  
Jeremy J. Selva ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Plasmodium Falciparum ◽  
Southeast Asia ◽  
Active Metabolite ◽  
Parasite Clearance ◽  
Ring Stage ◽  
Wild Type ◽  
Content Type ◽  
Kelch 13

ABSTRACT Artemisinin (ART) resistance has spread through Southeast Asia, posing a serious threat to the control and elimination of malaria. ART resistance has been associated with mutations in the Plasmodium falciparum kelch-13 ( Pfk13 ) propeller domain. Phenotypically, ART resistance is defined as delayed parasite clearance in patients due to the reduced susceptibility of early ring-stage parasites to the active metabolite of ART dihydroartemisinin (DHA). Early rings can enter a state of quiescence upon DHA exposure and resume growth in its absence. These quiescent rings are referred to as dormant rings or DHA-pretreated rings (here called dormant rings). The imidazolopiperazines (IPZ) are a novel class of antimalarial drugs that have demonstrated efficacy in early clinical trials. Here, we characterized the stage of action of the IPZ GNF179 and evaluated its activity against rings and dormant rings in wild-type and ART-resistant parasites. Unlike DHA, GNF179 does not induce dormancy. We show that GNF179 is more rapidly cidal against schizonts than against ring and trophozoite stages. However, with 12 h of exposure, the compound effectively kills rings and dormant rings of both susceptible and ART-resistant parasites within 72 h. We further demonstrate that in combination with ART, GNF179 effectively prevents recrudescence of dormant rings, including those bearing pfk13 propeller mutations.

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