scholarly journals Can malaria rapid diagnostic tests by drug sellers under field conditions classify children 5 years old or less with or without Plasmodium falciparum malaria? Comparison with nested PCR analysis

2018 ◽  
Vol 17 (1) ◽  
Author(s):  
Freddy Eric Kitutu ◽  
Henry Wamani ◽  
Katarina Ekholm Selling ◽  
Fred Ashaba Katabazi ◽  
Ronald Bisaso Kuteesa ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
Nested Pcr ◽  
Diagnostic Tests ◽  
Plasmodium Falciparum Malaria ◽  
Rapid Diagnostic Tests ◽  
Field Conditions ◽  
Pcr Analysis ◽  
Malaria Rapid Diagnostic Tests ◽  
Drug Sellers

scholarly journals Comparison of Capillary Versus Venous Blood for the Diagnosis of Plasmodium falciparum Malaria Using Rapid Diagnostic Tests

2021 ◽  
Author(s):  
Abalinda M Gorret ◽  
Rabbison Muhindo ◽  
Emma Baguma ◽  
Moses Ntaro ◽  
Edgar M Mulogo ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
Diagnostic Tests ◽  
Venous Blood ◽  
Plasmodium Falciparum Malaria ◽  
Rapid Diagnostic Tests ◽  
Sample Type ◽  
Malaria Rapid Diagnostic Tests ◽  
Polymerase Chain ◽  
Western Uganda

Abstract We enrolled 250 febrile children in western Uganda to compare the results of malaria rapid diagnostic tests (RDTs) when using capillary vs venous blood. Participants were tested with 4 different RDT types. Polymerase chain reaction testing was performed as the reference standard. Sensitivity and specificity were broadly similar across RDT types and sampling method. Agreement between sample type was high, ranging from 0.95 to 0.99. When following the manufacturer’s recommended interpretation, only 5 tests would have resulted in a different clinical diagnosis. These results demonstrate that malaria RDTs perform similarly when using capillary or venous blood in febrile children with Plasmodium falciparum malaria.

scholarly journals Assessing the impact of next-generation rapid diagnostic tests on Plasmodium falciparum malaria elimination strategies

Nature ◽  
2015 ◽  
Vol 528 (7580) ◽  
pp. S94-S101 ◽  
Author(s):  
Hannah C. Slater ◽  
Amanda Ross ◽  
André Lin Ouédraogo ◽  
Lisa J. White ◽  
Chea Nguon ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
Malaria Elimination ◽  
Diagnostic Tests ◽  
Plasmodium Falciparum Malaria ◽  
Rapid Diagnostic Tests ◽  
Next Generation ◽  
The Impact

scholarly journals Evaluation of a Colorimetric PCR-Based Assay To Diagnose Plasmodium falciparum Malaria in Travelers

1999 ◽  
Vol 37 (2) ◽  
pp. 339-341 ◽  
Author(s):  
Kathleen J. Y. Zhong ◽  
Kevin C. Kain
Keyword(s):  
Plasmodium Falciparum ◽  
Falciparum Malaria ◽  
Nested Pcr ◽  
Diagnostic Tests ◽  
Colorimetric Assay ◽  
Plasmodium Falciparum Malaria ◽  
Specific Method ◽  
Signal System ◽  
Reference Standard ◽  
Sharp Signal

New diagnostic tests are needed to facilitate the diagnosis ofPlasmodium falciparum malaria in the returned traveler. We performed a blinded evaluation of a nonisotopic colorimetric PCR-based assay (Digene SHARP Signal System) and compared the results with those obtained by microscopy and nested PCR for the detection of P. falciparum malaria in 150 febrile travelers. By using nested PCR as the reference standard, the colorimetric assay had a sensitivity of 100% and a specificity of 95.4% for the detection of P. falciparum. This PCR-based nonisotopic assay is a rapid, sensitive, and specific method for the detection of falciparum malaria in returned travelers.

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