scholarly journals Infection prevalence and ecotypes of Anaplasma phagocytophilum in moose Alces alces, red deer Cervus elaphus, roe deer Capreolus capreolus and Ixodes ricinus ticks from Norway

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Vetle M. Stigum ◽  
Ryanne I. Jaarsma ◽  
Hein Sprong ◽  
Christer M. Rolandsen ◽  
Atle Mysterud
Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 968
Author(s):  
Cornelia Silaghi ◽  
Julia Fröhlich ◽  
Hubert Reindl ◽  
Dietmar Hamel ◽  
Steffen Rehbein

(1) Background: Wild cervids play an important role in transmission cycles of tick-borne pathogens; however, investigations of tick-borne pathogens in sika deer in Germany are lacking. (2) Methods: Spleen tissue of 74 sympatric wild cervids (30 roe deer, 7 fallow deer, 22 sika deer, 15 red deer) and of 27 red deer from a farm from southeastern Germany were analyzed by molecular methods for the presence of Anaplasma phagocytophilum and Babesia species. (3) Results: Anaplasma phagocytophilum and Babesia DNA was demonstrated in 90.5% and 47.3% of the 74 combined wild cervids and 14.8% and 18.5% of the farmed deer, respectively. Twelve 16S rRNA variants of A. phagocytophilum were delineated. While the infection rate for A. phagocytophilum among the four cervid species was similar (71.4% to 100%), it varied significantly for Babesia between roe deer (73.3%), fallow deer (14.3%), sika deer (27.3%) and red deer (40.0%). Deer ≤2 years of age tested significantly more often positive than the older deer for both A. phagocytophilum and Babesia species. (4) Conclusions: This study confirms the widespread occurrence of A. phagocytophilum and Babesia species in wild cervids and farmed red deer in Germany and documents the co-occurrence of the two tick-borne pathogens in free-ranging sika deer.


Biologia ◽  
2011 ◽  
Vol 66 (6) ◽  
Author(s):  
Katarína Štefanidesová ◽  
Eva Špitalská ◽  
Ivan Krkoš ◽  
Elena Smetanová ◽  
Elena Kocianová

AbstractTick-borne bacterial zoonoses of livestock and free-ranging ungulates caused by Anaplasma spp. are common in Central Europe. The aim of this study was to analyze the prevalence of Anaplasma phagocytophilum and other tick-borne bacteria in wild animals from western Slovakia. Infection with A. phagocytophilum was recorded in 62.86% of analyzed roe deer (Capreolus capreolus), in two red deer (Cervus elaphus) and two wild boars (Sus scrofa). Dermacentor reticulatus and Ixodes ricinus ticks collected on red deer were not A. phagocytophilum-infected. However, spotted fever group rickettsiae were detected in ticks collected from red deer. High prevalence of A. phagocytophilum in roe deer as well as positive red deer and wild boars suggest the occurrence of natural foci in western Slovakia.


2008 ◽  
Vol 53 (No. 12) ◽  
pp. 668-675 ◽  
Author(s):  
B. Skotarczak ◽  
M. Adamska ◽  
M. Sawczuk ◽  
A. Maciejewska ◽  
B. Wodecka ◽  
...  

Molecular studies enabling the recognition of the role of game and ticks in the circulation of pathogens transmitted by ticks and detection of coinfections in order to estimate a risk which a contact with tissues of roe deer, red deer and wild boar from north-western Poland brings were the aim of this research. DNA isolated from the blood and spleen of game and from <i>Ixodes ricinus</i> were the study materials. The results shows that <i>Capreolus capreolus</i> and <i>Cervus elaphus</i> play an important role in the life cycle of <i>Anaplasma phagocytophilum</i>, two <i>Bartonella</i> species, <i>Theileria</i> and <i>Babesia</i> spp. Whereas in the isolates obtained from 50 representatives of <i>Sus scrofa</i>, the DNA of only one pathogen, <i>A. phagocytophilum</i> occurred. 63.5% of 74 PCR+ isolates from <i>Capreolus capreolus</i> showed a double coinfection and three isolates – triple. In the tissues of <i>Cervus elaphus</i>, the coinfections were triple in 38% of individuals, double in 40%, single in 84%.


2014 ◽  
Vol 172 (1-2) ◽  
pp. 272-278 ◽  
Author(s):  
Magdalena Rzewuska ◽  
Lucjan Witkowski ◽  
Agata A. Cisek ◽  
Ilona Stefańska ◽  
Dorota Chrobak ◽  
...  

2014 ◽  
Vol 17 (2) ◽  
pp. 315-319 ◽  
Author(s):  
A. Bancerz-Kisiel ◽  
A. Szczerba-Turek ◽  
A. Platt-Samoraj ◽  
P. Socha ◽  
W. Szweda

AbstractFree-living animals are an important environmental reservoir of pathogens dangerous for other animal species and humans. One of those is Yersinia (Y.) enterocolitica, the causative agent of yersiniosis - foodborne, enzootic disease, significant for public health. The purpose of the study was to identify bioserotypes and virulence markers of Y. enterocolitica strains isolated from roe deer (Capreolus capreolus) and red deer (Cervus elaphus) obtained during the 2010/2011 hunting season in north-eastern Poland. From among 48 rectal swabs obtained from 24 roe deer, two strains of Y. enterocolitica from one animal were isolated. Although both belonged to biotype 1A they were identified as different serotypes. The strain obtained from cold culture (PSB) belonged to serotype O:5, while the strain isolated from warm culture (ITC) was regarded as nonidentified (NI), what may suggest mixed infection in that animal. The presence of ystB gene, coding for YstB enterotoxin, directly related to Y. enterocolitica pathogenicity was detected in both strains using triplex PCR. The effect of the examination of 32 swabs obtained from 16 red deer was the isolation of two Y. enterocolitica strains from two different animals. Both belonged to biotype 1A with NI serotype, but were originated from different types of culture. They gave positive results in case of products of a size corresponding to the ystB gene. No amplicons corresponding to ail and ystA genes were found. Roe deer and red deer may carry and shed Y. enterocolitica, what seems to be important in aspect of an environmental reservoir of this pathogen. The Y. enterocolitica strains isolated from wild ruminants had the amplicons of the ystB gene, what suggest they can be potential source of Y. enterocolitica infection for humans


2019 ◽  
Vol 12 (1) ◽  
pp. 302-310
Author(s):  
A. Lauková ◽  
E. Bino ◽  
I. Kubašová ◽  
V. Strompfová ◽  
R. Miltko ◽  
...  

2008 ◽  
Vol 86 (3) ◽  
pp. 170-176 ◽  
Author(s):  
I. Barja ◽  
S. Rosellini

We examined whether group size in red deer ( Cervus elaphus L., 1758) and roe deer ( Capreolus capreolus (L., 1758)) under predation risk by Iberian wolves ( Canis lupus L., 1758) is affected by the type of habitat in which the deer reside. We hypothesized that group size (i) would be larger in open than in closed habitats, since it is an antipredator response, and (ii) would vary more with habitat type in the species that had higher wolf predation rates. In the study area, wolves were the only predator of wild ungulates, with roe deer being the main target prey. We performed monthly transects along paths to observe the group size of red and roe deer. In roe deer, the mean group size was significantly higher in open than in closed habitats, serving as an antipredator response. However, in red deer, habitat type did not affect group size. The results indicate that under predation risk by wolves the habitat type influences the grouping behavior of roe deer but not red deer. Furthermore, compared with forests, heaths offer less protection from predators and species in this habitat would benefit from larger group sizes.


2012 ◽  
Vol 56 (1) ◽  
pp. 15-19
Author(s):  
Małgorzata Adamska

Abstract The aim of this study was to describe the state of infection of roe deer (Capreolus capreolus) and red deer (Cervus elaphus) by Bartonella sp. in North-Western Poland through PCR detection of Bartonella 16S-23S rRNA ITS region in isolates of animal tissues, and also to describe the genetic diversity of detected Bartonella species based on molecular analysis of ITS. The multiple alignment analysis of ITS sequences was carried out, and homology matrices and phylogenetic trees were constructed. The DNA of Bartonella sp. was detected in tissues of 45.6% (36/79) C. capreolus and of 50% (15/30) C. elaphus. Products of two different sizes were detected: 317 bp, characteristic for B. schoenbuchensis, and 198 bp, characteristic for B. bovis. The obtained results suggest that roe and red deer are potential reservoirs of Bartonella sp. Most of the analysed ITS sequences was not specific for one host species. In constructed phylogenetic trees, sequences obtained from roe and red deer clustered together. These results suggest a lack of host specificity of most detected B. schoenbuchensis and B. bovis intraspecies strains


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