scholarly journals miR-17 promotes expansion and adhesion of human cord blood CD34+ cells in vitro

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Yuxia Yang ◽  
Saifeng Wang ◽  
Zhenchuan Miao ◽  
Wei Ma ◽  
Yanju Zhang ◽  
...  
Keyword(s):  
Cord Blood ◽  
Human Cord Blood ◽  
Cd34 Cells ◽  
Cord Blood Cd34

The αIIbβ3 integrin and GPIb-V-IX complex identify distinct stages in the maturation of CD34+cord blood cells to megakaryocytes

Blood ◽  
2000 ◽  
Vol 96 (13) ◽  
pp. 4169-4177 ◽  
Author(s):  
Adeline Lepage ◽  
Marylène Leboeuf ◽  
Jean-Pierre Cazenave ◽  
Corinne de la Salle ◽  
François Lanza ◽  
...  
Keyword(s):  
Cord Blood ◽  
Messenger Rna ◽  
Human Cord Blood ◽  
Cd34 Cells ◽  
Conditional Expression ◽  
Multistep Process ◽  
Cord Blood Cd34 ◽  
Specific Proteins ◽  
Cord Blood Cells

Abstract Megakaryocytopoiesis is a complex multistep process involving cell division, endoreplication, and maturation and resulting in the release of platelets into the blood circulation. Megakaryocytes (MK) progressively express lineage-restricted proteins, some of which play essential roles in platelet physiology. Glycoprotein (GP)Ib-V-IX (CD42) and GPIIb (CD41) are examples of MK-specific proteins having receptor properties essential for platelet adhesion and aggregation. This study defined the progressive expression of the GPIb-V-IX complex during in vitro MK maturation and compared it to that of GPIIb, an early MK marker. Human cord blood CD34+ progenitor cells were cultured in the presence of cytokines inducing megakaryocytic differentiation. GPIb-V-IX expression appeared at day 3 of culture and was strictly dependent on MK cytokine induction, whereas GPIIb was already present in immature CD34+ cells. Analysis by flow cytometry and of the messenger RNA level both showed that GPV appeared 1 day later than GPIb-IX. Microscopy studies confirmed the late appearance of GPV, which was principally localized in the cytoplasm when GPIb-IX was found on the cell surface, suggesting a delayed program of GPV synthesis and trafficking. Cell sorting studies revealed that the CD41+GPV+ population contained 4N and 8N cells at day 7, and was less effective than CD41+GPV− cells in generating burst-forming units of erythrocytes or MK colonies. This study shows that the subunits of the GPIb-V-IX complex represent unique surface markers of MK maturation. The genes coding for GPIb-IX and GPV are useful tools to study megakaryocytopoiesis and for tissue-specific or conditional expression in mature MK and platelets.

scholarly journals In vitro differentiation of natural killer T cells from human cord blood CD34+ cells

2003 ◽  
Vol 121 (1) ◽  
pp. 148-156 ◽  
Author(s):  
So-Youn Woo ◽  
Yu-Jin Jung ◽  
Kyung-Ha Ryu ◽  
Hae-Young Park ◽  
Jeong-Hae Kie ◽  
...  
Keyword(s):  
T Cells ◽  
Cord Blood ◽  
Natural Killer ◽  
Natural Killer T Cells ◽  
In Vitro Differentiation ◽  
Human Cord Blood ◽  
Cd34 Cells ◽  
Cord Blood Cd34 ◽  
Killer T Cells

Extensive generation of human cord blood CD34+ stem cells from Lin−CD34− cells in a long-term in vitro system

2000 ◽  
Vol 28 (6) ◽  
pp. 690-699 ◽  
Author(s):  
Kiyoshi Ando ◽  
Yoshihiko Nakamura ◽  
Jamel Chargui ◽  
Hideyuki Matsuzawa ◽  
Takashi Tsuji ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Human Cord Blood ◽  
In Vitro System ◽  
Cd34 Cells ◽  
Cord Blood Cd34

Ultrastructural and phenotypic analysis of in vitro erythropoiesis from human cord blood CD34+ cells

2003 ◽  
Vol 82 (5) ◽  
pp. 278-283 ◽  
Author(s):  
J.-H. Kie ◽  
Y.-J. Jung ◽  
S.-Y. Woo ◽  
K.-H. Ryu ◽  
H.-Y. Park ◽  
...  
Keyword(s):  
Cord Blood ◽  
Phenotypic Analysis ◽  
Human Cord Blood ◽  
Cd34 Cells ◽  
Cord Blood Cd34

The αIIbβ3 integrin and GPIb-V-IX complex identify distinct stages in the maturation of CD34+cord blood cells to megakaryocytes

Blood ◽  
2000 ◽  
Vol 96 (13) ◽  
pp. 4169-4177 ◽  
Author(s):  
Adeline Lepage ◽  
Marylène Leboeuf ◽  
Jean-Pierre Cazenave ◽  
Corinne de la Salle ◽  
François Lanza ◽  
...  
Keyword(s):  
Cord Blood ◽  
Messenger Rna ◽  
Human Cord Blood ◽  
Cd34 Cells ◽  
Conditional Expression ◽  
Multistep Process ◽  
Cord Blood Cd34 ◽  
Specific Proteins ◽  
Cord Blood Cells

Megakaryocytopoiesis is a complex multistep process involving cell division, endoreplication, and maturation and resulting in the release of platelets into the blood circulation. Megakaryocytes (MK) progressively express lineage-restricted proteins, some of which play essential roles in platelet physiology. Glycoprotein (GP)Ib-V-IX (CD42) and GPIIb (CD41) are examples of MK-specific proteins having receptor properties essential for platelet adhesion and aggregation. This study defined the progressive expression of the GPIb-V-IX complex during in vitro MK maturation and compared it to that of GPIIb, an early MK marker. Human cord blood CD34+ progenitor cells were cultured in the presence of cytokines inducing megakaryocytic differentiation. GPIb-V-IX expression appeared at day 3 of culture and was strictly dependent on MK cytokine induction, whereas GPIIb was already present in immature CD34+ cells. Analysis by flow cytometry and of the messenger RNA level both showed that GPV appeared 1 day later than GPIb-IX. Microscopy studies confirmed the late appearance of GPV, which was principally localized in the cytoplasm when GPIb-IX was found on the cell surface, suggesting a delayed program of GPV synthesis and trafficking. Cell sorting studies revealed that the CD41+GPV+ population contained 4N and 8N cells at day 7, and was less effective than CD41+GPV− cells in generating burst-forming units of erythrocytes or MK colonies. This study shows that the subunits of the GPIb-V-IX complex represent unique surface markers of MK maturation. The genes coding for GPIb-IX and GPV are useful tools to study megakaryocytopoiesis and for tissue-specific or conditional expression in mature MK and platelets.

In vitro clinical-grade generation of red blood cells from human umbilical cord blood CD34+ cells

Transfusion ◽  
2008 ◽  
Vol 48 (10) ◽  
pp. 2235-2245 ◽  
Author(s):  
Eun Jung Baek ◽  
Han-Soo Kim ◽  
Sinyoung Kim ◽  
Honglien Jin ◽  
Tae-Yeal Choi ◽  
...  
Keyword(s):  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Cells ◽  
Human Umbilical Cord Blood ◽  
Human Umbilical Cord ◽  
Clinical Grade ◽  
Cd34 Cells ◽  
Cord Blood Cd34

Myelotoxicity of trichothecenes and apoptosis: An in vitro study on human cord blood CD34+ hematopoietic progenitor

2005 ◽  
Vol 19 (8) ◽  
pp. 1015-1024 ◽  
Author(s):  
G. Le Dréan ◽  
M. Auffret ◽  
P. Batina ◽  
F. Arnold ◽  
Y. Sibiril ◽  
...  
Keyword(s):  
Cord Blood ◽  
In Vitro Study ◽  
Vitro Study ◽  
Hematopoietic Progenitor ◽  
Human Cord Blood ◽  
Cord Blood Cd34

Evaluation of Reliable Reference Genes for In Vitro Erythrocyte Generation from Cord Blood CD34+ Cells

2021 ◽  
Author(s):  
Lei Xu ◽  
Zhan Gao ◽  
Zhou Yang ◽  
Mingyi Qu ◽  
Huilin Li ◽  
...  
Keyword(s):  
Cord Blood ◽  
Reference Genes ◽  
Cd34 Cells ◽  
Cord Blood Cd34
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