scholarly journals Carboxymethyl cellulase production optimization from newly isolated thermophilic Bacillus subtilis K-18 for saccharification using response surface methodology

AMB Express ◽  
2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Muhammad Irfan ◽  
Qudsia Mushtaq ◽  
Fouzia Tabssum ◽  
Hafiz Abdullah Shakir ◽  
Javed Iqbal Qazi
Keyword(s):  
Response Surface Methodology ◽  
Bacillus Subtilis ◽  
Response Surface ◽  
Cellulase Production ◽  
Production Optimization ◽  
Thermophilic Bacillus ◽  
Carboxymethyl Cellulase

Media Optimization for Cellulase Production at Low Energy Consumption with Response Surface Methodology

2012 ◽  
Vol 34 (20) ◽  
pp. 1883-1892 ◽  
Author(s):  
M. Yang ◽  
D. D. Fan ◽  
Y.-E. Luo ◽  
Y. Mi ◽  
J. Hui ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Energy Consumption ◽  
Response Surface ◽  
Cellulase Production ◽  
Low Energy ◽  
Media Optimization ◽  
Low Energy Consumption

Optimizing the biodegradation of two keratinous wastes through a Bacillus subtilis recombinant strain using a response surface methodology

2010 ◽  
Vol 21 (6) ◽  
pp. 1077-1092 ◽  
Author(s):  
Amira M. Embaby ◽  
Taha I. Zaghloul ◽  
Ahmed R. Elmahdy
Keyword(s):  
Response Surface Methodology ◽  
Bacillus Subtilis ◽  
Response Surface ◽  
Recombinant Strain

scholarly journals Response surface methodology-artificial neural network based optimization and strain improvement of cellulase production by Streptomyces sp.

2020 ◽  
Vol 36 (4) ◽  
Author(s):  
Ega Soujanya Lakshmi ◽  
Manda Rama Narasinga Rao ◽  
Muddada Sudhamani
Keyword(s):  
Neural Network ◽  
Artificial Neural Network ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Wild Strain ◽  
Cellulase Activity ◽  
Cellulase Production ◽  
Optimal Conditions ◽  
Zone Formation ◽  
Artificial Neural

ABSTRACT Thirty seven different colonies were isolated from decomposing logs of textile industries. From among these, a thermotolerant, grampositive, filamentous soil bacteria Streptomyces durhamensis vs15 was selected and screened for cellulase production. The strain showed clear zone formation on CMC agar plate after Gram’s iodine staining.  Streptomyces durhamensis vs15 was further confirmed for cellulase production by estimating the reducing sugars through dinitrosalicylic acid (DNS) method. The activity was enhanced by sequential mutagenesis using three mutagens of ultraviolet irradiation (UV), N methyl-N’-nitro-N-nitrosoguanidine (NTG) and Ethyl methane sulphonate (EMS). After mutagenesis, the cellulase activity of GC23 (mutant) was improved to 1.86 fold compared to the wild strain (vs15). Optimal conditions for the production of cellulase by the GC 23 strain were evaluated using Response Surface Methodology (RSM) and Artificial Neural Network (ANN). Effect of pH, temperature, duration of incubation, , and substrate concentration on cellulase production were evaluated. Optimal conditions for the production of cellulase enzyme using Carboxy Methyl Cellulase as a substrate are 55 oC of temperature, pH of 5.0 and incubation for 40 h. The cellulase activity of the mutant Streptomyces durhamensis GC23 was further optimised to 2 fold of the activity of the wild type by RSM and ANN.  

Medium optimization for the production of thermal stable β-glucanase by Bacillus subtilis ZJF-1A5 using response surface methodology

2004 ◽  
Vol 93 (2) ◽  
pp. 175-181 ◽  
Author(s):  
Xing-Jun Tang ◽  
Guo-Qing He ◽  
Qi-He Chen ◽  
Xiu-Yan Zhang ◽  
Mokhtar A.M Ali
Keyword(s):  
Response Surface Methodology ◽  
Bacillus Subtilis ◽  
Response Surface ◽  
Medium Optimization ◽  
Thermal Stable

Optimization of Medium Constituents for the Production of Fructosyltransferase (Ftase) by Bacillus subtilis Using Response Surface Methodology

2008 ◽  
Vol 3 (3) ◽  
pp. 114-121 ◽  
Author(s):  
I. Sarat Babu . ◽  
S. Ramappa . ◽  
D. Guru Mahesh . ◽  
K. Sunanda Kumari . ◽  
K. Sita Kumari . ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Bacillus Subtilis ◽  
Response Surface
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