Recombinant Laccase Production Optimization in Pichia pastoris by Response Surface Methodology and Its Application in the Biodegradation of Octyl Phenol and 4-Tert-Octylphenol

2021 ◽  
Author(s):  
Qi Li ◽  
Changsheng Chai ◽  
Yitong Du ◽  
Junli Cai ◽  
Linguo Zhao
Keyword(s):  
Response Surface Methodology ◽  
Pichia Pastoris ◽  
Response Surface ◽  
Production Optimization ◽  
Laccase Production ◽  
Recombinant Laccase ◽  
Octyl Phenol

Media Optimization for Laccase Production by Trichoderma harzianum ZF-2 Using Response Surface Methodology

2013 ◽  
Vol 23 (12) ◽  
pp. 1757-1764 ◽  
Author(s):  
Huiju Gao ◽  
Xiang Chu ◽  
Yanwen Wang ◽  
Fei Zhou ◽  
Kai Zhao ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Trichoderma Harzianum ◽  
Laccase Production ◽  
Media Optimization

Production Optimization of Alkali-Thermo Tolerant Crude Endoglucanase from Funalia leonina by Response Surface Methodology

2017 ◽  
Vol 113 (09) ◽  
pp. 1750
Author(s):  
Varun Kumar ◽  
Nirmal Sudhir Kumar Harsh ◽  
Sanjay Naithani ◽  
Bipin Prakash Thapliyal ◽  
Shrikant Sharma
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Production Optimization

scholarly journals Chitinase from a Novel Strain ofSerratia marcescensJPP1 for Biocontrol of Aflatoxin: Molecular Characterization and Production Optimization Using Response Surface Methodology

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Kai Wang ◽  
Pei-sheng Yan ◽  
Li-xin Cao
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Pcr Amplification ◽  
Fold Increase ◽  
Antagonistic Activity ◽  
Medium Composition ◽  
Production Optimization ◽  
Reading Frame ◽  
Mycolytic Enzymes ◽  
Burman Design

Chitinase is one of the most important mycolytic enzymes with industrial significance, and produced by a number of organisms. A chitinase producing isolateSerratia marcescensJPP1 was obtained from peanut hulls in Jiangsu Province, China, and exhibited antagonistic activity against aflatoxins. In this study, we describe the optimization of medium composition with increased production of chitinase for the selected bacteria using statistical methods: Plackett-Burman design was applied to find the key ingredients, and central composite design of response surface methodology was used to optimize the levels of key ingredients for the best yield of chitinase. Maximum chitinase production was predicted to be 23.09 U/mL for a 2.1-fold increase in medium containing 12.70 g/L colloidal chitin, 7.34 g/L glucose, 5.00 g/L peptone, 1.32 g/L (NH4)2SO4, 0.7 g/L K2HPO4, and 0.5 g/L MgSO4·7H2O. Polymerase chain reaction (PCR) amplification of the JPP1 chitinase gene was performed and obtained a 1,789 bp nucleotide sequence; its open reading frame encoded a protein of 499 amino acids named as ChiBjp.

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