Isotopic Assessment of Groundwater Salinity: A Case Study of the Southwest (SW) Region of Punjab, India

In recent decades, due to rapid increases in water demand and greater usage of water for irrigation from surface canals, waterlogging problems have been created in the southwest zone of Punjab, coupled with a stagnation in saline zone formation due to salinity ingression. To understand these salinity issues, the present study has been conducted in three districts (Muktsar, Fazilka, and Faridkot) of Punjab to understand the root cause. To this end, groundwater samples were collected from 142 piezometers developed at 40 sites. Electrical conductivity (EC) observations were taken in the field, and collected samples were analyzed for isotopes in the laboratory. Results found that salinity in groundwater arises from the combination of evaporation enrichment and salt dissolution. The dissolved salts may be acquired due to salts from aquifer materials or salts from surface soils dissolving and leaching down with the recharging water. Besides, the zone of interaction is mapped using stable isotopic composition. The study suggests that zone of interaction between aquifers can be effectively used in groundwater augmentation, management, and contamination control at regional and/or global scales to curb water demand in the future.

Contrasting transform and passive margin subsidence history and heat flow evolution: insights from 3D thermo-mechanical modelling

Transform and passive margins developed during the continental rifting and opening of oceanic basins are fundamental elements of plate tectonics. It has been suggested that inherited structures, plate divergence velocities and surface processes exert a first order control on the topographic and bathymetric evolution and thermal history of these margins and related sedimentary basins. Their complex spatial-temporal dynamics have remained controversial. Here, we conducted 3D magmatic-thermo-mechanical numerical experiments coupled with surface processes modelling to simulate the dynamics of continental rifting, continental transform fault zone formation and persistent oceanic transform faulting and zero-offset oceanic fracture zones development. Numerical modelling results allow to explain the first order observations from passive and transform margins, such as diachronous rifting, heat flow rise and cooling in individual depocenters and contrasting basin tectonics of extensional and transtensional origin. In addition, the models reproduce the rise of both marginal ridges and transform marginal plateaus and their interaction with erosion and sedimentation. Comparison of model results with observations from natural examples yield new insights into the tectono-sedimentary and thermal evolution of several key passive and transform continental margins worldwide.Supplementary material at https://doi.org/10.6084/m9.figshare.c.5756555

Antibacterial, Antifungal, and Antioxidant Activities of Silver Nanoparticles Biosynthesized from Bauhinia tomentosa Linn

The biogenic synthesis of silver nanoparticles (AgNPs) has a wide range of applications in the pharmaceutical industry. Here, we synthesized AgNPs using the aqueous flower extract of Bauhinia tomentosa Linn. Formation of AgNPs was observed using ultraviolet-visible light spectrophotometry at different time intervals. Maximum absorption was observed after 4 h at 420 nm due to the reduction of Ag+ to Ag0. The stabilizing activity of functional groups was identified by Fourier-transform infrared spectroscopy. Size and surface morphology were also analyzed using scanning electron microscopy. The present study revealed the AgNPs were spherical in form with a diameter of 32 nm. The face-centered cubic structure of AgNPs was indexed using X-ray powder diffraction with peaks at 2θ = 37°, 49°, 63°, and 76° (corresponding to the planes of silver 111, 200, 220, 311), respectively. Energy-dispersive X-ray spectroscopy revealed that pure reduced silver (Ag0) was the major constituent (59.08%). Antimicrobial analyses showed that the biosynthesized AgNPs possess increased antibacterial activity (against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative), with larger zone formation against S. aureus (9.25 mm) compared with that of E. coli (6.75 mm)) and antifungal activity (against Aspergillus flavus and Candida albican (with superior inhibition against A. flavus (zone of inhibition: 7 mm) compared with C. albicans (zone of inhibition: 5.75 mm)). Inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity was found to be dose-dependent with half-maximal inhibitory concentration (IC50) values of 56.77 μg/mL and 43.03 μg/mL for AgNPs and ascorbic acid (control), respectively, thus confirming that silver nanoparticles have greater antioxidant activity than ascorbic acid. Molecular docking was used to determine the mode of antimicrobial interaction of our biosynthesized B. tomentosa Linn flower-powder extract-derived AgNPs. The biogenic AgNPs preferred hydrophobic contacts to inhibit bacterial and fungal sustainability with reducing antioxidant properties, suggesting that biogenic AgNPs can serve as effective medicinal agents.

Loss of CEP162 function at the primary cilium delays ciliogenesis and causes retinal ciliopathy in humans

Ciliopathies often comprise retinal degeneration since the photoreceptor outer segment is an adapted primary cilium. CEP162 is a distal end centriolar protein required for proper transition zone assembly during ciliogenesis and whose loss causes ciliopathy in zebrafish. CEP162 has so far not been implicated in human disease. Here, we identified a homozygous CEP162 frameshift variant, c.1935dupA (p.(E646R*5)), in retinitis pigmentosa patients from two unrelated Moroccan families, likely representing a founder allele. We found that even though mRNA levels were reduced, the truncated CEP162-E646R*5 protein was expressed and localized to the mitotic spindle during mitosis, but not at the basal body of the cilium. In CEP162 knockdown cells, expression of the truncated CEP162-E646R*5 protein is unable to restore ciliation indicating its loss of function at the cilium. In patient fibroblasts, cilia overcome the absence of CEP162 from the primary cilium by delaying ciliogenesis through the persistence of CP110 at the mother centriole. The patient fibroblasts are ultimately able to extend some abnormally long cilia that are missing key transition zone components. Defective transition zone formation likely disproportionately affects the long-living ciliary outer segment of photoreceptors resulting in retinal dystrophy. CEP162 is expressed in human retina, and we show that wild-type CEP162, but not truncated CEP162-E646R*5, specifically localizes to the distal end of centrioles of mouse photoreceptor cilia. Together, our genetic, cell-based, and in vivo modeling establish that CEP162 deficiency causes retinal ciliopathy in humans.

Exploring cellulolytic microorganisms from coffee industry by-products and their enzyme properties

Cellulolytic microorganism has immense potential due to their cellulase production, enzyme complexity and widespread habitat of life. This study was conducted to obtain microbial cellulase with vast industrial applicability from the coffee industry by-product in East Java, Indonesia. Fifty-four isolates with significant clear zone formation were obtained by Congo red staining in CMC agar plates. Eighteen bacteria, two yeasts and two moulds with high cellulolytic index were subjected to protein content determination as well as reducing sugar analysis in various conditions such as pH, temperature, addition of metal ions, surfactant and inhibitor agent. The specific activity measurements of all the crude enzymes result in the highest value of cellulase activity produced by isolate C12 which was 0.401 ± 0.018 U/mg. This enzyme activity was known to be optimum at 50°C and pH 9. It was also stimulated by K+, Na+, Mg2+, Fe3+, and SDS. However, the enzyme activity was inhibited by EDTA at 10 mM concentration. The use of coffee industry by-products as the source of cellulolytic microorganisms offers a promising approach for its various types of indigenous microorganisms and their unique property of cellulase produced that is useful for industrial application.

Dynamic Model and Numerical Simulation of Maximum Turbidity Zone Formation in River Inlet

The estuary of a river can be seen as a relatively free and partially closed coastal body. It is connected to the ocean and is a transitional zone of rivers, which contains processes from land to sea and from fresh water to salt water. The estuary is one of the most productive natural habitats in the world and carries a large number of sediments due to natural factors such as changes in runoff and tides. Therefore, many coastal areas with river estuaries have become the most densely populated areas in the human population. In this paper, the RSM (Reynolds stress model) turbulence model and the PID (proportional integral derivative) algorithm are successfully used to simulate the dynamic model and for the numerical simulation of the formation of turbidity maximum zone in the estuary, which provides a theoretical basis for the follow-up of the similar research studies.

Quantitative Evaluation of Soil Structure and Strain in Three Dimensions under Shear Using X-ray Computed Tomography Image Analysis

The objective of this study is to quantitatively evaluate the soil structure behavior when under shear stress to understand the mechanism of shear zone formation using a micro-focus X-ray computed tomography (CT) scanner to visualize the internal samples without causing disturbance. A new image-analysis method was proposed to systematically evaluate the particle length and direction by fitting the particle as an ellipsoid. Subsequently, a direct shear experiment was conducted on soil materials, and shear band was scanned using a micro-focus X-ray CT scanner. After validating the proposed method, the soil structure was evaluated in the shear zone via image analysis on the CT images. Furthermore, the strain inside the specimen was evaluated using digital image correlation. The results showed that a partial change in the particle direction occurred when the volume expansion inside the shear zone exceeded the peak. In addition, the width of the shear zone was ~7.1 times the median grain size of the sand used; however, the region exhibiting a change in the direction of the particles was narrow and confined to the vicinity of the shear plane.

Isolation and characterization of phosphate solubilizing bacteria in saline soil from Costal Region of Odisha

The present study was conducted to isolate phosphate solubilizing bacteria (PSB) from rhizospheric saline soils of coastal Odisha, India and evaluated their phosphate solubilizing ability. Total four PSB were isolated based on the halo zone formation (solubilizing index 2.63-3.14) on PVK agar medium and were characterized based on biochemical and molecular characteristics as Bacillus subtilis (B1), B. megaterium (B2), Sphingomonas paucimobilis (P2) and Kocuria kristinae (P6). The inorganic phosphate released by PSB ranged from 18.532 to 38.250µg/ml with decreasing the pH PVK broth up to 3.9. Acid phosphatase activity for PSB were recorded 84.237-98.658µmol/min. Glucose was found to be the best carbon source for B. subtilis, Sphingomonas paucimobilis and Kocuria kristinae whereas mannitol for B. megaterium. Optimum acid phosphatase activity was observed for all the four PSB isolates in presence of ammonium sulphate as nitrogen source in PVK broth at 30oC and pH 7.0.

Rapid fragmentation of Thwaites Eastern Ice Shelf, West Antarctica

Ice shelves play a key role in the dynamics of marine ice sheets, by buttressing grounded ice and limiting rates of ice flux to the oceans. In response to recent climatic and oceanic change, ice shelves fringing the West Antarctic Ice Sheet (WAIS) have begun to fragment and retreat, with major implications for ice sheet stability. Here, we focus on the Thwaites Eastern Ice Shelf (TEIS), the remaining pinned floating extension of Thwaites Glacier. We show that TEIS has undergone a process of fragmentation in the last five years, including brittle failure along a major shear zone, formation of tensile cracks on the main body of the shelf, and release of tabular bergs on both eastern and western flanks. Simulations with the Helsinki Discrete Element Model (HiDEM) show that this pattern of failure is associated with high backstress from a submarine pinning point at the distal edge of the shelf. We show that a significant zone of shear upstream of the main pinning point developed in response to the rapid acceleration of the shelf between 2002 and 2006, seeding damage on the shelf. Subsequently, basal melting and positive feedbacks between damage and strain rates weakened TEIS, allowing damage to accumulate. Thus, although backstress on TEIS has likely diminished through time as the pinning point has shrunk, accumulation of damage has ensured that the ice in the shear zone has remained the weakest link in the system. Experiments with the BISICLES ice sheet model indicate that additional damage to or unpinning of TEIS are unlikely to trigger significantly increased ice loss from WAIS, but the calving response to loss of TEIS remains highly uncertain. It is widely recognised that ice-shelf fragmentation and collapse can be triggered by hydrofracturing and/or unpinning from ice shelf margins or grounding points. Our results indicate a third mechanism, backstress-triggered failure, that can occur when ice ffractures in response to stresses associated with pinning points. In most circumstances, pinning points are essential for ice shelf stability, but as ice shelves thin and weaken the concentration of backstress in damaged ice upstream of a pinning point may provide the seeds of their demise.