scholarly journals Myostatin gene promoter: structure, conservation and importance as a target for muscle modulation

Author(s):  
Carla Vermeulen Carvalho Grade ◽  
Carolina Stefano Mantovani ◽  
Lúcia Elvira Alvares
1984 ◽  
Vol 259 (14) ◽  
pp. 8764-8768
Author(s):  
J A Mankovich ◽  
P H Lai ◽  
N Gokul ◽  
J Konisky

2011 ◽  
Vol 363 (1-2) ◽  
pp. 291-299 ◽  
Author(s):  
Bing Deng ◽  
Jianghui Wen ◽  
Yi Ding ◽  
Qishuang Gao ◽  
Haijun Huang ◽  
...  

Endocrinology ◽  
1999 ◽  
Vol 140 (12) ◽  
pp. 5598-5608 ◽  
Author(s):  
David F. Gordon ◽  
Whitney W. Woodmansee ◽  
Suzanne R. Lewis ◽  
R. Andrew James ◽  
William M. Wood ◽  
...  

2007 ◽  
Vol 50 (5) ◽  
pp. 648-654 ◽  
Author(s):  
Rong Du ◽  
XiaoRong An ◽  
YongFu Chen ◽  
Jian Qin

2007 ◽  
Vol 6 (sup1) ◽  
pp. 109-109
Author(s):  
S. Dall’Olio ◽  
A. Falaschini ◽  
M. Tassinari ◽  
G. Canestrari

2002 ◽  
Vol 22 (20) ◽  
pp. 7066-7082 ◽  
Author(s):  
Michael P. Spiller ◽  
Ravi Kambadur ◽  
Ferenc Jeanplong ◽  
Mark Thomas ◽  
Julie K. Martyn ◽  
...  

ABSTRACT Myostatin is a negative regulator of myogenesis, and inactivation of myostatin leads to heavy muscle growth. Here we have cloned and characterized the bovine myostatin gene promoter. Alignment of the upstream sequences shows that the myostatin promoter is highly conserved during evolution. Sequence analysis of 1.6 kb of the bovine myostatin gene upstream region revealed that it contains 10 E-box motifs (E1 to E10), arranged in three clusters, and a single MEF2 site. Deletion and mutation analysis of the myostatin gene promoter showed that out of three important E boxes (E3, E4, and E6) of the proximal cluster, E6 plays a significant role in the regulation of a reporter gene in C2C12 cells. We also demonstrate by band shift and chromatin immunoprecipitation assay that the E6 E-box motif binds to MyoD in vitro and in vivo. Furthermore, cotransfection experiments indicate that among the myogenic regulatory factors, MyoD preferentially up-regulates myostatin promoter activity. Since MyoD expression varies during the myoblast cell cycle, we analyzed the myostatin promoter activity in synchronized myoblasts and quiescent “reserve” cells. Our results suggest that myostatin promoter activity is relatively higher during the G1 phase of the cell cycle, when MyoD expression levels are maximal. However, in the reserve cells, which lack MyoD expression, a significant reduction in the myostatin promoter activity is observed. Taken together, these results suggest that the myostatin gene is a downstream target gene of MyoD. Since the myostatin gene is implicated in controlling G1-to-S progression of myoblasts, MyoD could be triggering myoblast withdrawal from the cell cycle by regulating myostatin gene expression.


Sign in / Sign up

Export Citation Format

Share Document