Effect of dietary supplementation with Spirulina on the expressions of AANAT, ADRB3, BTG2 and FASN genes in the subcutaneous adipose and Longissimus dorsi muscle tissues of purebred and crossbred Australian sheep

AbstractIn order to detect the molecular mechanism of heterosis in pigs, an mRNA differential display (DD) technique was performed to investigate the differences in gene expression in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs. Fourteen expressed sequence tags (ESTs), differentially expressed between the hybrid and purebred pigs, were isolated and identified through semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Nucleotide sequence analysis revealed that the 14 ESTs are not homologous to any of the known genes or ESTs. These novel ESTs were then deposited in the GenBank database. Tissue expression profile analysis showed that the ESTs were expressed in most tissues, including heart, spleen, liver, kidney, small intestine, ovary and lung, and this also implied that these genes must be important for the life process. Our results indicate the diversity of differential display of genes between the hybrids and purebreds in the Meishan×Large White cross combination. Results also suggest that heterosis in pigs might be derived from the differential expression of many indispensable genes in specific life phases.

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Lipids deposition, composition and oxidative stability of subcutaneous adipose tissue and Longissimus dorsi muscle in Guizhou mini-pig at different developmental stages

Meat Science ◽

10.1016/j.meatsci.2009.11.003 ◽

2010 ◽

Vol 84 (4) ◽

pp. 684-690 ◽

Cited By ~ 6

Author(s):

Yong Yang ◽

Chunjiang Zhao ◽

Shan Xiao ◽

He Zhan ◽

Min Du ◽

...

Keyword(s):

Adipose Tissue ◽

Oxidative Stability ◽

Subcutaneous Adipose Tissue ◽

Developmental Stages ◽

Longissimus Dorsi ◽

Longissimus Dorsi Muscle ◽

Dorsi Muscle ◽

Subcutaneous Adipose

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Isolation, Sequence Analysis and Expression Profile of a Novel Swine Gene Differentially Expressed in the Longissimus Dorsi Muscle Tissues from Landrace×Large White Cross-combination

Acta Biochimica et Biophysica Sinica ◽

10.1093/abbs/37.3.186 ◽

2005 ◽

Vol 37 (3) ◽

pp. 186-191 ◽

Cited By ~ 16

Author(s):

Yong-Gang Liu ◽

Yuan-Zhu Xiong ◽

Chang-Yan Deng

Keyword(s):

Carbonic Anhydrase ◽

Differentially Expressed ◽

Longissimus Dorsi ◽

Cross Combination ◽

Large White ◽

Phylogenetic Tree Analysis ◽

Longissimus Dorsi Muscle ◽

Carbonic Anhydrase Iii ◽

Dorsi Muscle ◽

Muscle Tissues

Abstract The mRNA differential display technique was performed to investigate the differences in gene expression in the Longissimus dorsi muscle tissues from Landrace×Large White cross-combination. One novel gene that was differentially expressed was identified using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and its complete cDNA sequence was obtained using the rapid amplification of cDNA ends (RACE) method. The nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 260 amino acids that contains the putative conserved domain of the carbonic anhydrase, and this protein has high homology with the carbonic anhydrase III (CA-III) of four species-mouse (91%), horse (91%), rat (89%) and human (86%)–so that it can be defined as swine carbonic anhydrase III. The phylogenetic tree analysis revealed that the swine CA-III has a closer genetic relationship with the horse CA-III than with those of mouse, rat and human. The tissue expression analysis indicated that the swine CA-III gene is generally expressed in most tissues. Our experiment is the first to establish the primary foundation for further research on the swine CA-III gene.

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Isolation, cDNA sequence analysis and tissue expression profile of a novel swine gene differentially expressed in the Longissimus dorsi muscle tissues from Large White × Meishan cross combination

Progress in Natural Science Materials International ◽

10.1080/10020070512331343187 ◽

2005 ◽

Vol 15 (12) ◽

pp. 1073-1078

Author(s):

Liu Yonggang ◽

Lei Minggang ◽

Xiong Yuanzhu ◽

Deng Changyan

Keyword(s):

Cdna Sequence ◽

Tissue Expression ◽

Longissimus Dorsi ◽

Cross Combination ◽

Large White ◽

Longissimus Dorsi Muscle ◽

Dorsi Muscle ◽

Muscle Tissues ◽

Tissue Expression Profile ◽

Cdna Sequence Analysis

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Low Dietary n-6/n-3 PUFA Ratio Regulates Meat Quality, Reduces Triglyceride Content, and Improves Fatty Acid Composition of Meat in Heigai Pigs

Animals ◽

10.3390/ani10091543 ◽

2020 ◽

Vol 10 (9) ◽

pp. 1543

Author(s):

Qiuyun Nong ◽

Liyi Wang ◽

Yanbing Zhou ◽

Ye Sun ◽

Wentao Chen ◽

...

Keyword(s):

Fatty Acid ◽

Meat Quality ◽

Dietary Supplementation ◽

Finishing Pigs ◽

Pufa Ratio ◽

Longissimus Dorsi Muscle ◽

Dorsi Muscle ◽

Triglyceride Content ◽

Subcutaneous Adipose ◽

Fatty Acid Compositions

The objective of this study was to investigate the effects of dietary supplementation with different n-6/n-3 polyunsaturated fatty acid (PUFA) ratios on growth performance, meat quality, and fatty acid profile in Heigai pigs. A total of 54 Heigai finishing pigs (body weight: 71.59 ± 2.16 kg) were randomly divided into three treatments with six replications (three pigs per replication) and fed diets containing different n-6/n-3 PUFA ratios: 8:1, 5:1, and 3:1. Pigs fed the dietary n-6/n-3 PUFA ratio of 8:1 had the highest feed to gain ratio (p < 0.01), carcass weight (p < 0.05), redness a* (p < 0.01), and yellowness b* (p < 0.01). Fatty acid compositions in longissimus dorsi muscle (LDM) and subcutaneous adipose tissue (SAT) were significantly changed (p < 0.01). Notably, the meat from the pigs fed with the low dietary n-6/n-3 PUFA ratio had higher n-3 PUFA contents (p < 0.01) and lower n-6/n-3 PUFA ratio (p < 0.01). The triglyceride and total cholesterol contents were significantly decreased in SAT from the pigs fed with dietary n-6/n-3 PUFA ratios of 5:1 (p < 0.05) and 3:1 (p < 0.01). Reducing n-6/n-3 PUFA ratio upregulated the expression of HSL (p < 0.05), CPT1 (p < 0.01), and FABP4 (p < 0.01) but downregulated ATGL (p < 0.01) expression. These results demonstrate that the lower n-6/n-3 PUFA ratio regulates meat quality and enhances the deposition of n-3 PUFA in Heigai pigs.

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