scholarly journals Pathogenicity of the entomopathogenic fungus, Beauveria bassiana (Bals.) Vuill. endophytic and a soil isolate against the squash beetle, Epilachna chrysomelina (F.) (Coleoptera: Coccinellidae)

Author(s):  
Feyroz Ramadan Hassan ◽  
Samir Khalaf Abdullah ◽  
Lazgeen Haji Assaf

AbstractLaboratory and field bioassays were conducted to evaluate the pathogenicity of an endophytic and a soil isolate of the entomopathogenic fungus, Beauveria bassiana (Bals.) Vuill. against different stages of squash beetle, Epilachna chrysomelina (F.) (Coleoptera: Coccinellidae). Both isolates were identified by ITS rDNA sequence analysis. Both isolates were pathogenic to the squash beetle; however, their potential was different according to the conidia concentration and the exposure period. Three days post treatment, (100%) mortality rate was obtained, when the first and second larval instars were treated by the B. bassiana ES (soil isolate) compared to 83.67 and 72.60%, respectively when treated with the endophytic isolate. A percentage of 17.67% malformation occurred among the adults that emerged from treated pupae. The highest mortality percentage under field conditions were 28.67 and 22.33% for larvae and adults, respectively.

Author(s):  
Seher Uçar ◽  
Turgut Atay ◽  
Yusuf Yanar

In this work, control capacity of the four isolates (GN22-1, HP15, HP5-2, HP3-1) of entomopathogenic fungus Beauveria bassiana Bals. (Vuill) were evaluated against Tribolium castaneum (Herbst, 1797) (Coleoptera: Tenebrionidae) adults under laboratory conditions. To test the effect of each of the isolate on adults of T. castaneum were dipped into 1×108 conidia/ml suspension of each isolate. The data for mortality was recorded after 3rd, 5th, 7th, 9th, 11th, 13th, 15th, 17th, 19th, 21st, and 23rd day. Thirteen days after application, isolates were listed as GN22-1 (72.85%), HP3-1 (48.88%), HP15 (47.37%) and HP5-2 (30.43%) based on the mortality rate they caused. Mortality rate was 83.52% at the end of the 23rd day with isolate GN22-1. While HP3-1 (53.74%) and HP15 (52.24%) caused more than 50% effect at the end of 23 days incubation period, the effect of HP5-2 remained only 32.51%. In addition, LT50 and LT90 rates were also determined. We arrive to the conclusion that especially GN22-1 isolate can has a potential in the control of this insect and may serve an alternative to chemical insecticides.


2021 ◽  
Vol 499 (1) ◽  
pp. 260-265
Author(s):  
L. F. Ashmarina ◽  
G. R. Lednev ◽  
O. G. Tomilova ◽  
T. A. Sadokhina ◽  
D. Yu. Bakshaev ◽  
...  

2019 ◽  
Vol 29 (1) ◽  
Author(s):  
Monir M. M. El Husseini

AbstractLarval and adult populations of the Egyptian alfalfa weevil (EAW) Hypera brunneipennis (Boheman) (Coleoptera: Curculionidae) was monitored after application of the entomopathogenic fungus Beauveria bassiana in the alfalfa field (Medicago sativa L.) in two successive seasons 2014/2015 and 2015/2016. The second and last generation of the weevil on April 10, 2016, was controlled by only one application with the conidiospores of the entomopathogenic fungus B. bassiana (3 × 108 spores/ml). Accordingly, the larval population decreased from 16.07 ± 1.09 in season 2015/2016 to 7.37 ± 0.05 individuals/50 sweep net double strokes in season 2016/2017. Also, the adult weevil’s population decreased from 5.66 ± 0.8 to 2.55 ± 0.6 individuals/50 sweep net double strokes in the two seasons, respectively. 39.66% mortality rate was recorded in the Hypera brunneipennis adults aestivated under loose bark of the surrounding eucalyptus trees, which received the application of B. bassiana in the field. Another application with the fungus, targeting the second generation of the pest adults in alfalfa each season, will undoubtedly lead to a further decrease in the pest population.


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