scholarly journals Biochemical characterization of spiromesifen and spirotetramat as lipid synthesis inhibitors on cotton leaf worm, Spodoptera littoralis

Author(s):  
Ragaei M. Abdel-Fatah ◽  
Samy M. Mohamed ◽  
Atef A. Aly ◽  
Al-Kazafy H. Sabry
2022 ◽  
Vol 11 (1) ◽  
pp. 63-68 ◽  
Author(s):  
Omar M. Elhady ◽  
Erian. S. Mansour ◽  
M. M. Elwassimy ◽  
Sameh A. Zawam ◽  
Ali M. Drar

Many of mimic analogues synthesized before depending on the change in the structure of aromatic rings. In this work, the carbonyl group in the structure of compounds 1-4 converted to thiocarbonyl group, and then studying the toxicological activity due to chemical change in the active center of mimic analogues was performed for compounds N-tert-butyl-2,4-dichloro-N'-(2,4-dichlorobenzoyl)benzohydrazide (2) and N-tert-butyl-2,4-dichloro-N'-[(2,4-dichlorophenyl)carbonothioyl]benzenecarbothiohydrazide (6). The toxicological study was done by using 2nd and 4th instar larvae of the cotton leaf worm, Spodoptera littoralis (Boisd.). Five concentration levels (600, 300, 150, 75 and 37.5 ppm) of compounds (2) and (6) were applied on the fresh plant food to the newly grown (2nd and 4th) instar larvae.


2020 ◽  
Author(s):  
Ouma Onguka ◽  
Brett M. Babin ◽  
Markus Lakemeyer ◽  
Ian T. Foe ◽  
Neri Amara ◽  
...  

SummaryThe intracellular protozoan parasite Toxoplasma gondii must scavenge cholesterol and other lipids from the host to facilitate intracellular growth and replication. Enzymes responsible for neutral lipid synthesis have been identified but there is no evidence for enzymes that catalyze lipolysis of cholesterol esters and esterified lipids. Here we characterize several T. gondii serine hydrolases with esterase and thioesterase activities that were previously thought to be depalmitoylating enzymes. We find they do not cleave palmitoyl thiol esters but rather hydrolyze short chain lipid esters. Deletion of one of the hydrolases results in alterations in levels of multiple lipids species. We also identify small molecule inhibitors of these hydrolases and show that treatment of parasites results in phenotypic defects reminiscent of parasites exposed to excess cholesterol or oleic acid. Together, these data characterize enzymes necessary for processing lipids critical for infection and highlight the potential for targeting parasite hydrolases for therapeutic applications.HighlightsBioinformatic and biochemical characterization of T. gondii serine hydrolases reveals substrate preference between enzymes with similar catalytic foldT. gondii serine hydrolases previously thought to be depalmitoylases are lipid metabolizing enzymesT. gondii lipid metabolism pathways utilize enzymes that are viable therapeutic targets


2015 ◽  
Vol 13 (4) ◽  
pp. 3-9
Author(s):  
Muhammad Azmat Ullah Khan ◽  
Ahmad Ali Shahid ◽  
Abdul Qayyum Rao ◽  
Kamran Shehzad Bajwa ◽  
Tahir Rehman Samiullah ◽  
...  

2013 ◽  
Vol 1830 (6) ◽  
pp. 3734-3744 ◽  
Author(s):  
Debojit Guha ◽  
C.G. Poornima Priyadarshini ◽  
Arunima Purakayastha ◽  
R. Thippeswamy ◽  
M. Lakshmikanth ◽  
...  

2017 ◽  
Vol 8 (1) ◽  
pp. 34-47 ◽  
Author(s):  
Ahmad Alsayed Om ◽  
Azza Hosni Moha ◽  
Mahmoud Imam Nasr ◽  
Khalil Abdel Hame ◽  
Elsayed Abdel Khal ◽  
...  

Author(s):  
J. H. Resau ◽  
N. Howell ◽  
S. H. Chang

Spinach grown in Texas developed “yellow spotting” on the peripheral portions of the leaves. The exact cause of the discoloration could not be determined as there was no evidence of viral or parasitic infestation of the plants and biochemical characterization of the plants did not indicate any significant differences between the yellow and green leaf portions of the spinach. The present study was undertaken using electron microscopy (EM) to determine if a micro-nutrient deficiency was the cause for the discoloration.Green leaf spinach was collected from the field and sent by express mail to the EM laboratory. The yellow and equivalent green portions of the leaves were isolated and dried in a Denton evaporator at 10-5 Torr for 24 hrs. The leaf specimens were then examined using a JEOL 100 CX analytical microscope. TEM specimens were prepared according to the methods of Trump et al.


2014 ◽  
Vol 3 (3) ◽  
pp. 218-225
Author(s):  
R. G. Somkuwar ◽  
M. A. Bhange ◽  
A. K. Upadhyay ◽  
S. D. Ramteke

SauvignonBlanc wine grape was characterized for their various morphological, physiological and biochemical parameters grafted on different rootstocks. Significant differences were recorded for all the parameters studied. The studies on vegetative parameters revealed that the rootstock influences the vegetative growth thereby increasing the photosynthetic activities of a vine. The highest photosynthesis rate was recorded in 140-Ru grafted vine followed by Fercal whereas the lowest in Salt Creek rootstock grafted vines.The rootstock influenced the changes in biochemical constituents in the grafted vine thereby helping the plant to store enough food material. Significant differences were recorded for total carbohydrates, proteins, total phenols and reducing sugar. The vines grafted on1103-Pshowed highest carbohydrates and starch followed by 140-Ru,while the least amount of carbohydrates were recorded in 110-R and Salt Creek grafted vines respectively.Among the different rootstock graft combinations, Fercal showed highest amount of reducing sugar, proteins and phenols, followed by 1103-P and SO4, however, the lowest amount of reducing sugar, proteins and phenols were recorded with 110-R grafted vines.The vines grafted on different rootstocks showed changes in nutrient uptake. Considering this, the physico-biochemical characterization of grafted vine may help to identify particularrootstocks combination that could influence a desired trait in commercial wine grape varieties after grafting.


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