Use of systematic analysis of DNA repair pathways in head and neck cancer (HNC) to identify XPF as a novel predictor of induction response, and pMK2 relationship to chemoradiotherapy

2008 ◽  
Vol 26 (15_suppl) ◽  
pp. 6003-6003 ◽  
Author(s):  
T. Y. Seiwert ◽  
E. E. Cohen ◽  
X. Wang ◽  
M. Kocherginsky ◽  
M. Bhayani ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Systematic Analysis ◽  
Repair Pathways

DNA repair pathways to regulate response to chemoradiotherapy in patients with locally advanced head and neck cancer

Tumor Biology ◽  
2016 ◽  
Vol 37 (10) ◽  
pp. 13435-13443 ◽  
Author(s):  
B. Cirauqui ◽  
M. Margelí ◽  
V. Quiroga ◽  
A. Quer ◽  
N. Karachaliou ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Locally Advanced ◽  
Advanced Head ◽  
Repair Pathways

Use of DNA repair pathway analysis to predict overall survival in head and neck cancer patients treated with TFHX chemoradiotherapy

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 6033-6033
Author(s):  
T. Seiwert ◽  
D. T. Weaver ◽  
X. Wang ◽  
J. Kutok ◽  
E. Cohen ◽  
...  
Keyword(s):  
Overall Survival ◽  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Excision Repair ◽  
Chemotherapeutic Agents ◽  
Tumor Cell Death ◽  
Survival Group ◽  
Repair Pathways

6033 Background: Concurrent chemoradiotherapy is a standard-of-care for head and neck cancer. Radiotherapy and chemotherapeutic agents damage DNA; lack of adequate repair induces tumor cell death. The network of DNA repair pathways was examined to predict TFHX chemoradiotherapy outcomes. Methods: Biopsy specimens (paraffin) from 60 HNC patients were evaluated from tissue microarrays. Samples originated from patient groups from phase I/II studies: 1) poor-prognosis radiation-naïve, 2) re-irradiation. All were treated with TFHX-based chemoradiotherapy. DNA repair biomarkers XPF, pMK2, PAR, pH2AX, FANCD2, ATM, BRCA1, RAD51, ERCC1 (clone 8F1), and p53 were explored using IHC, automated image processing, and machine reading (>500 cells/read), for nuclear and cytoplasmic quantity, area, and intensity, and correlated with clinical outcome. Results: There was low inter-core variability per tumor with median patient ranking signal to noise ratio of 15.0 across all markers. Patients were stratified into short and long survival groups by determination of critical marker thresholds. Five DNA repair biomarkers (RAD51, ATM, BRCA1, XPF, FANCD2) exhibited univariate significance for overall survival (p = 2.65e-3, 1.53e-2, 4.77e-4, 8.57e-5, 1.32e-3)(additional clinical parameters multivariate analysis was not feasible due to sample size). ERCC1 was not statistically significant (p = 1.0). Pairwise biomarker combinations improved survival group discrimination. Combination of 4 DNA repair biomarkers (FANCD2, BRCA1, ATM, XPF) was also significant (p = 1.31e-4). Discriminant analysis demonstrated higher fractions of correctly identified patients in good/poor survival groups from four-marker tests. Conclusions: Five DNA repair biomarkers predicted overall survival following TFHX-based chemoradiotherapy. By contrast ERCC1 (8F1) was not significant. Combination of markers improved predictive ability in this study. The analysis of DNA repair pathways, particularly in homologous recombination, DNA damage response, and nucleotide excision repair, may be clinically useful. Validation in a larger, homogeneous patient population, and using platinum-based chemoradiotherapy, is indicated and currently ongoing. [Table: see text]

scholarly journals DNA Repair in Lymphoblastoid Cell Lines From Patients With Head and Neck Cancer

1999 ◽  
Vol 125 (2) ◽  
pp. 185 ◽  
Author(s):  
Erich M. Sturgis ◽  
Gary L. Clayman ◽  
Yongli Guan ◽  
Zhaozheng Guo ◽  
Qingyi Wei
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cell Lines ◽  
Neck Cancer ◽  
Lymphoblastoid Cell Lines

DNA repair gene polymorphisms and risk of head and neck cancer in the Tunisian population

2013 ◽  
Vol 43 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Rim Khlifi ◽  
Imen Kallel ◽  
Bouthaina Hammami ◽  
Amel Hamza-Chaffai ◽  
Ahmed Rebai
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Gene Polymorphisms ◽  
Tunisian Population ◽  
Repair Gene ◽  
Dna Repair Gene

DNA repair- and apoptosis-pathways to regulate response to chemo-radiotherapy (CRT) in patients (p) with locally advanced head and neck cancer (HNC).

2015 ◽  
Vol 33 (15_suppl) ◽  
pp. e17025-e17025
Author(s):  
Beatriz Cirauqui ◽  
Vanesa Quiroga ◽  
Imane Chaib ◽  
Belen Sanchez ◽  
Niki Karachaliou ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Locally Advanced ◽  
Advanced Head ◽  
Apoptosis Pathways

Role of impaired DNA repair in genotoxic susceptibility of patients with head and neck cancer

2008 ◽  
Vol 25 (5) ◽  
pp. 489-497 ◽  
Author(s):  
Pawel Rusin ◽  
Jurek Olszewski ◽  
Alina Morawiec-Bajda ◽  
Karolina Przybylowska ◽  
Dariusz Kaczmarczyk ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer

scholarly journals Genetic polymorphisms in key DNA repair genes and risk of head and neck cancer in a Chinese population

2012 ◽  
Vol 3 (4) ◽  
pp. 719-724 ◽  
Author(s):  
HUA YUAN ◽  
HUIZHANG LI ◽  
HONGXIA MA ◽  
YUMING NIU ◽  
YUNONG WU ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Genetic Polymorphisms ◽  
Neck Cancer ◽  
Chinese Population ◽  
Dna Repair Genes ◽  
Repair Genes

The Expression of Checkpoint and DNA Repair Genes in Head and Neck Cancer as Possible Predictive Factors

2016 ◽  
Vol 23 (2) ◽  
pp. 253-264 ◽  
Author(s):  
Orsolya Rusz ◽  
Margit Pál ◽  
Éva Szilágyi ◽  
László Rovó ◽  
Zoltán Varga ◽  
...  
Keyword(s):  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Neck Cancer ◽  
Predictive Factors ◽  
Dna Repair Genes ◽  
Repair Genes

DNA Damage and Repair of Head and Neck Cancer Cells after Radio- and Chemotherapy

2009 ◽  
Vol 64 (7-8) ◽  
pp. 601-610 ◽  
Author(s):  
Pawel Rusin ◽  
Anna Walczak ◽  
Anita Zwierzchlejska ◽  
Jurek Olszewski ◽  
Alina Morawiec-Bajda ◽  
...  
Keyword(s):  
Dna Damage ◽  
Dna Repair ◽  
Head And Neck Cancer ◽  
Head And Neck ◽  
Cancer Cells ◽  
Neck Cancer ◽  
Cancer Cell Line ◽  
Repair Capacity ◽  
Dna Damage And Repair ◽  
Γ Radiation

DNA repair is critical for successful chemo- and radiotherapy of human tumours, because their genotoxic sensitivity may vary in different types of cancer cells. In this study we have compared DNA damage and the efficiency of its repair after genotoxic treatment with hydrogen peroxide, cisplatin and γ-radiation of head and neck squamous cell carcinoma (HNSCC). Lymphocytes and tissue cells from biopsies of 37 cancer patients and 35 healthy donors as well as the HTB-43 larynx cancer cell line were employed. The cell sensitivity to genotoxic treatment was estimated by the MTT survival assay. The extent of DNA damage and efficiency of its repair was examined by the alkaline comet assay. Among the examined treatments, we found that HNSCC cells were the most sensitive to γ-radiation and displayed impaired DNA repair. In particular, DNA damage was repaired less effectively in cells from HNSCC metastasis than healthy controls. In conclusion, our results suggest that the different genotoxic sensitivity of HNSCC cells may depend on their DNA repair capacity what in turn may be connected with the effectiveness of head and neck cancer therapy.

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