Finite Proliferative Capacity of Syrian Hamster Fetal and Adult Fibroblasts in Vitro: A Model System for the Analysis of the Cellular Basis of Aging

2020 ◽  
pp. 203-219
Author(s):  
Sarah A. Bruce
Keyword(s):  
Syrian Hamster ◽  
Model System ◽  
Proliferative Capacity ◽  
Cellular Basis ◽  
Adult Fibroblasts

In Vitro Differentiation of Monocytoid THP-1 Cells Affects Their Permissiveness for HIV Strains: A Model System for Studying the Cellular Basis of HIV Differential Tropism

Virology ◽  
1993 ◽  
Vol 193 (1) ◽  
pp. 256-267 ◽  
Author(s):  
Pascal R.A. Meylan ◽  
Celsa A. Spina ◽  
Douglas D. Richman ◽  
Richard S. Kornbluth
Keyword(s):  
Model System ◽  
In Vitro Differentiation ◽  
Cellular Basis

EXPANSION OF HEMATOPOIETIC STEM CELLS IN VITRO AS A MODEL SYSTEM FOR HUMAN TISSUE ENGINEERING

2000 ◽  
Vol 31 (3) ◽  
pp. 499-509 ◽  
Author(s):  
Joel S. Greenberger ◽  
Julie P. Goff ◽  
Jason Bush ◽  
Alfred Bahnson ◽  
Douglas Koebler ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Hematopoietic Stem Cells ◽  
Human Tissue ◽  
Model System ◽  
Hematopoietic Stem

scholarly journals Exposure of ichnovirus particles to digitonin leads to enhanced infectivity and induces fusion from without in an in vitro model system

2007 ◽  
Vol 88 (11) ◽  
pp. 2977-2984 ◽  
Author(s):  
Don Stoltz ◽  
Renée Lapointe ◽  
Andrea Makkay ◽  
Michel Cusson
Keyword(s):  
Outer Membrane ◽  
In Vitro Model ◽  
Model System ◽  
Host Cells ◽  
Fusion Event ◽  
Susceptible Host ◽  
In Vitro Model System ◽  
Vitro Model

Unlike most viruses, the mature ichnovirus particle possesses two unit membrane envelopes. Following loss of the outer membrane in vivo, nucleocapsids are believed to gain entry into the cytosol via a membrane fusion event involving the inner membrane and the plasma membrane of susceptible host cells; accordingly, experimentally induced damage to the outer membrane might be expected to increase infectivity. Here, in an attempt to develop an in vitro model system for studying ichnovirus infection, we show that digitonin-induced disruption of the virion outer membrane not only increases infectivity, but also uncovers an activity not previously associated with any polydnavirus: fusion from without.

Effect of Oxidized Myofibrils Protein Subjected to Mutiple Freeze-Thaw Cycles on N-Nitrosamine Formation in In Vitro Model System

2012 ◽  
Vol 550-553 ◽  
pp. 1590-1594 ◽  
Author(s):  
Hua Yang ◽  
Pei Pei Meng ◽  
Rui Wang ◽  
Pei Ran Li ◽  
Peng Li ◽  
...  
Keyword(s):  
Sodium Nitrite ◽  
Protein Carbonyl ◽  
Model System ◽  
Model Systems ◽  
Secondary Amines ◽  
Freeze Thaw ◽  
Heavy Chains ◽  
Carcinogenic Substance ◽  
The Times

N-nitrosamine is a kind of carcinogenic substance, which is possibly formed in the reaction of nitrites with amino acids or secondary amines. Two in vitro model systems were designed to evaluate the influence of oxidized myofibrils protein subjected to repeated freeze-thaw cycles (0, 1, 2, 3, 4, 7, 10 times) on N-nitrosamine formation. Model system I contains diethylamine and sodium nitrite, while model system II contains only sodium nitrite as reaction solution. Oxidized myofibrils protein were added to both systems. The results revealed that as the number of freeze-thaw cycles increased, cross-linking of myosin heavy chains and the content of protein carbonyl increased, but the content of protein sulfydryl decreased, which indicates oxidization of protein occurred. The concentration of N-nitrosodiethylamine increased as the number of freeze-thaw cycles increased, especially after four cycles. Oxidized myofibrils protein promoted the formation of N-nitrosodiethylamine. The more the times of freeze-thaw cycles were subjected, the more oxidization of myofibrils protein occurred and the higher yield of the N-nitrosodiethylamine.

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