Alterations in Leydig Cell Morphology

Leydig cells are known to be the main source of steroids in testicles. The agranular endoplasmic reticulum which in Leydig cells is abundant, has been considered to be the source and place of metabolism of steroids. One of the methods utilized in the male to control human population growth, is the vasectomy. Since many vasectomized men had improved their sexual behaviour, a matter that according to Phoenix is mediated by psychological factors, the question, that if Leydig cells are modified after vasectomy is still unanswered. We decided to find out the possible changes in Leydig cells if any, after the vasectomy.

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Steroid biosynthesis and Leydig cell morphology in adult unilaterally cryptorchid rats

Acta Endocrinologica ◽

10.1530/acta.0.1070556 ◽

1984 ◽

Vol 107 (4) ◽

pp. 556-562 ◽

Cited By ~ 16

Author(s):

A. Bergh ◽

A. Åson Berg ◽

J.-E. Damber ◽

M. Hammar ◽

G. Selstam

Keyword(s):

Cell Morphology ◽

Leydig Cell ◽

Leydig Cells ◽

Lipid Droplets ◽

Steroid Biosynthesis ◽

Adult Age ◽

Unilateral Cryptorchidism ◽

Profile Area ◽

Cell Profile

Abstract. Unilateral cryptorchidism was induced in newborn rats and when the rats reached adult age Leydig cell morphology and steroid biosynthesis were compared in scrotal and abdominal testes. The cell profile area of abdominal Leydig cells was reduced, the cytoplasm contained an increased number of lipid droplets and the endoplasmatic reticulum was more sparse than in scrotal Leydig cells. The intratesticular concentrations of progesterone, 20α-dihydroprogesterone, 17α-hydroxyprogesterone and testosterone were measured both basally and after LH stimulation. The synthesis of testosterone was subnormal in the abdominal testes. The data on basal steroid concentrations suggest a block at the 17α-hydroxylase level, but observations after LH stimulation suggest that earlier steps are also involved. When studied in vitro the steroid biosynthesis in abdominal testes was also disturbed. The conversion mediated by 17α-hydroxylase and 17β-kestosteroid reductase was lower and the conversion mediated by 20α-dehydrogenase was higher in the abdominal testis. The reason for the disturbed steroid biosynthesis in abdominal testes is unknown. It may be related to the increased concentration of oestradiol in abdominal testes. Other possibilities are altered paracrine influence from the damaged tubules or changes in the microcirculation of the testis.

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Author response for "Differential temporal and spatial post‐injury alterations in cerebral cell morphology and viability"

10.1002/cne.24955/v3/response1 ◽

2020 ◽

Author(s):

Zareen Amtul ◽

Abdullah N. Najdat ◽

David J. Hill ◽

Edith J. Arany

Keyword(s):

Cell Morphology ◽

Author Response ◽

Post Injury ◽

Temporal And Spatial

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Cell morphology, volume, and surface area determinations from multilevel contouring within HVEM micrographs of serial sections and whole-cell mounts

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100127426 ◽

1987 ◽

Vol 45 ◽

pp. 588-589

Author(s):

M. Marko ◽

A. Leith ◽

D. Parsons

Keyword(s):

Surface Area ◽

Electron Micrographs ◽

Cell Morphology ◽

Individual Variation ◽

Serial Section ◽

Stereo Pair ◽

Complex Structures ◽

Serial Sections ◽

Surface Areas ◽

Computer Based

The use of serial sections and computer-based 3-D reconstruction techniques affords an opportunity not only to visualize the shape and distribution of the structures being studied, but also to determine their volumes and surface areas. Up until now, this has been done using serial ultrathin sections.The serial-section approach differs from the stereo logical methods of Weibel in that it is based on the Information from a set of single, complete cells (or organelles) rather than on a random 2-dimensional sampling of a population of cells. Because of this, it can more easily provide absolute values of volume and surface area, especially for highly-complex structures. It also allows study of individual variation among the cells, and study of structures which occur only infrequently.We have developed a system for 3-D reconstruction of objects from stereo-pair electron micrographs of thick specimens.

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