Characterization of the 2 M NaCl-Resistant Chromatin Fraction from Chicken Erythroid Cells

2018 ◽  
pp. 75-101
Author(s):  
Peter C. Hentzen ◽  
Isaae Bekhor
Keyword(s):  
Erythroid Cells ◽  
Chromatin Fraction

scholarly journals Generation and Characterization of Erythroid Cells from Human Embryonic Stem Cells and Induced Pluripotent Stem Cells: An Overview

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Kai-Hsin Chang ◽  
Halvard Bonig ◽  
Thalia Papayannopoulou
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Pluripotent Stem Cells ◽  
Embryonic Stem ◽  
Erythroid Cells ◽  
Hematopoietic Stem ◽  
Induced Pluripotent

Because of the imbalance in the supply and demand of red blood cells (RBCs), especially for alloimmunized patients or patients with rare blood phenotypes, extensive research has been done to generate therapeutic quantities of mature RBCs from hematopoietic stem cells of various sources, such as bone marrow, peripheral blood, and cord blood. Since human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) can be maintained indefinitely in vitro, they represent potentially inexhaustible sources of donor-free RBCs. In contrast to other ex vivo stem-cell-derived cellular therapeutics, tumorigenesis is not a concern, as RBCs can be irradiated without marked adverse effects on in vivo function. Here, we provide a comprehensive review of the recent publications relevant to the generation and characterization of hESC- and iPSC-derived erythroid cells and discuss challenges to be met before the eventual realization of clinical usage of these cells.

Molecular Characterization of Glycophorin A Transcripts in Human Erythroid Cells Using RT-PCR, Allele-Specific Restriction, and Sequencing

Vox Sanguinis ◽  
1995 ◽  
Vol 68 (2) ◽  
pp. 121-128 ◽  
Author(s):  
B.R. DuPont ◽  
S.G. Grant ◽  
S.H. Oto ◽  
W.L. Bigbee ◽  
R.H. Jensen ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Glycophorin A ◽  
Erythroid Cells ◽  
Rt Pcr ◽  
Allele Specific ◽  
Specific Restriction

Characterization of nuclear orphan receptor TR2/TR4 complexes in erythroid cells

2007 ◽  
Vol 38 (2) ◽  
pp. 149
Author(s):  
Katarzyna Kolodziej ◽  
Osamu Tanabe ◽  
Shoko Kobayashi ◽  
Jeroen Demmers ◽  
Frank Grosveld ◽  
...  
Keyword(s):  
Orphan Receptor ◽  
Erythroid Cells ◽  
Orphan Receptor Tr2 ◽  
Nuclear Orphan Receptor ◽  
In Erythroid Cells

Ultrastructural characterization of the erythroid cells in a novel case of congenital anemia

2003 ◽  
Vol 30 (1) ◽  
pp. 30-42 ◽  
Author(s):  
Marianna H Antonelou ◽  
Issidora S Papassideri ◽  
Fotini J Karababa ◽  
Afroditi Loutradi ◽  
Lukas H Margaritis
Keyword(s):  
Erythroid Cells ◽  
Ultrastructural Characterization

Erythroid cells in immunoregulation: characterization of a novel suppressor factor

2004 ◽  
Vol 93 (2-3) ◽  
pp. 171-178 ◽  
Author(s):  
Galina V Seledtsova ◽  
Victor I Seledtsov ◽  
Denis M Samarin ◽  
Vladimir V Senyukov ◽  
Irina P Ivanova ◽  
...  
Keyword(s):  
Erythroid Cells ◽  
Suppressor Factor

scholarly journals Characterization of Adult α- and β-Globin Genes in the Zebrafish

Blood ◽  
1997 ◽  
Vol 89 (2) ◽  
pp. 688-700 ◽  
Author(s):  
Fung-Yee Chan ◽  
Judith Robinson ◽  
Alison Brownlie ◽  
Ramesh A. Shivdasani ◽  
Adriana Donovan ◽  
...  
Keyword(s):  
High Performance ◽  
Globin Gene ◽  
Regulatory Elements ◽  
Reversed Phase ◽  
Cdna Clones ◽  
Globin Genes ◽  
Erythroid Cells ◽  
Insight Into ◽  
Globin Gene Expression

Abstract Developmental switching of hemoglobins (Hbs) occurs in most vertebrates, yet the cellular and molecular basis for this process remains elusive. The zebrafish is a new genetic and developmental system that can be used to study embryogenesis, and mutants with a variety of defects in hematopoiesis have recently been derived. To initiate our studies on Hb switching in this organism, we have characterized the globins expressed in the adult. Reversed-phase high performance liquid chromatography and mass spectrometric analyses of adult peripheral blood hemolysates showed that there are three major α globins and two β globins in circulating erythroid cells. In addition, we have isolated and characterized zebrafish adult α- and β-globin cDNA clones that encode some of these globins. High levels of α- and β-globin gene expression were detected in adult erythroid cells, whereas embryonic erythroid cells expressed little, if any, of these RNAs. We have also shown that the α- and β-globin genes are tightly linked on the same chromosome and are arrayed in a 3′-5′ to 5′-3′ configuration, respectively. The characterization of these genes and regulatory elements in this globin locus will provide insight into the process of globin gene transcription. With these reagents, future studies of Hb switching in zebrafish mutants with defective hematopoiesis will be possible.

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