Cytochrome P450 2C - a source of EDHF and reactive oxygen species in the porcine coronary artery

BACKGROUND: Fruits of Crataegus songarica are commonly used for the treatment of vascular insufficiency and heart problems. OBJECTIVE: Our aim was to determine the effect of C. songarica on vascular tone and to determine the mechanisms underlying the vasorelaxant properties. METHODS: Extracts of C. songarica were tested for vasodilator activity of porcine coronary artery after pre-contraction with the thromboxane mimetic U46619 in the presence or absence of inhibitors of intracellular signaling cascades. Reactive oxygen species were assessed by dihydroethidine staining and the level of eNOS and AKT phosphorylation was measured by immunohistochemical staining. RESULTS: Extracts of C. songarica berries produced endothelium dependent vasorelaxation, with most significant effect induced by aqueous fraction (AS-CS). This vasorelaxant effect of AS-CS was reduced by inhibition of nitric oxide pathways and inhibition of potassium channels. Inhibition of phosphatidylinositol 3- kinase and Src tyrosine kinase, as well as scavenging of reactive oxygen species, produced an attenuation of the relaxation response. Estrogen receptor antagonists (tamoxifen and ICI 182,782) reduced the AS-CS mediated vasorelaxation. AS-CS also stimulated the endothelial formation of ROS and phosphorylation of Akt and eNOS. CONCLUSION: The data indicated that C. songarica produces an endothelium-dependent vasorelaxation, which is partly dependent upon estrogen receptors, and sensitive to inhibition of ROS/Src/PI3K/NO pathways.

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HYPOXIC VASORELAXATION IN PORCINE CORONARY ARTERY IS NOT MEDIATED BY REACTIVE OXYGEN SPECIES

The FASEB Journal ◽

10.1096/fasebj.23.1_supplement.1032.1 ◽

2009 ◽

Vol 23 (S1) ◽

Author(s):

Richard J. Paul ◽

Peggy Sue Bowman ◽

Anthony J. Parr ◽

Yukisato Ishida ◽

Robert L. Wardle ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Coronary Artery ◽

Reactive Oxygen ◽

Oxygen Species ◽

Porcine Coronary Artery

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Increased reactive oxygen species generation in specific mutants of cytochrome P450 2D6

Free Radical Biology and Medicine ◽

10.1016/s0891-5849(98)90056-9 ◽

1998 ◽

Vol 25 ◽

pp. S22

Keyword(s):

Reactive Oxygen Species ◽

Cytochrome P450 ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Oxygen Species ◽

Cytochrome P450 2D6 ◽

P450 2D6

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The role of reactive oxygen species in the infarct-limiting effect of hypoxic preconditioning

Regional blood circulation and microcirculation ◽

10.24884/1682-6655-2021-20-2-87-91 ◽

2021 ◽

Vol 20 (2) ◽

pp. 87-91

Author(s):

A. S. Sementsov ◽

N. V. Naryzhnaya ◽

M. A. Sirotina ◽

L. N. Maslov

Keyword(s):

Reactive Oxygen Species ◽

Coronary Artery ◽

Ischemia Reperfusion ◽

Reactive Oxygen ◽

Coronary Artery Occlusion ◽

Artery Occlusion ◽

Hypoxic Preconditioning ◽

Male Rats ◽

Oxygen Species ◽

Infarct Size Reduction

Introduction. Increased resistance of the heart to ischemia/reperfusion (I/R) is an urgent aim of physiology, pharmacology, and cardiac surgery, since I/R injury of the heart is often the cause of cardiogenic shock and subsequent death of patients in the postoperative period. Materials and methods. The study was carried out in male rats which were subjected to coronary artery occlusion (45 min) and reperfusion (2 h). Before coronary occlusion, early hypoxic preconditioning (HP) was modeled. The rats were subjected to six sessions of hypoxia (8 % O2, 10 min) and reoxygenation (21 % O2, 10 min) 30 min before coronary artery occlusion. The rats were injected with the following drugs: 1,3-dimethylthiourea (DMTM), 2-mercaptopropionyl glycine (2-MPG), deferoxamine. Results. It was found that HP contributes to infarct size reduction by 30 %. Preliminary administration of DMTM, 2-MPG, deferoxamine eliminated the infarct-reducing effect of HP. Conclisuon. The obtained data indicate that reactive oxygen species are involved in the cardioprotective effect of HP.

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