scholarly journals HYPOXIC VASORELAXATION IN PORCINE CORONARY ARTERY IS NOT MEDIATED BY REACTIVE OXYGEN SPECIES

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Richard J. Paul ◽  
Peggy Sue Bowman ◽  
Anthony J. Parr ◽  
Yukisato Ishida ◽  
Robert L. Wardle ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Coronary Artery ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Porcine Coronary Artery

Hawthorn berry (crataegus songarica) causes endothelium-dependent relaxation of the porcine coronary artery: Role of Estrogen receptors

2021 ◽  
pp. 1-17
Author(s):  
Waqas Younis ◽  
Alamgeer ◽  
Valerie B. Schini-Kerth ◽  
Muhammad Akmal farooq ◽  
Musaad Althobaiti ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Coronary Artery ◽  
Estrogen Receptors ◽  
Intracellular Signaling ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Src Tyrosine Kinase ◽  
Aqueous Fraction ◽  
Porcine Coronary Artery ◽  
Vascular Insufficiency

BACKGROUND: Fruits of Crataegus songarica are commonly used for the treatment of vascular insufficiency and heart problems. OBJECTIVE: Our aim was to determine the effect of C. songarica on vascular tone and to determine the mechanisms underlying the vasorelaxant properties. METHODS: Extracts of C. songarica were tested for vasodilator activity of porcine coronary artery after pre-contraction with the thromboxane mimetic U46619 in the presence or absence of inhibitors of intracellular signaling cascades. Reactive oxygen species were assessed by dihydroethidine staining and the level of eNOS and AKT phosphorylation was measured by immunohistochemical staining. RESULTS: Extracts of C. songarica berries produced endothelium dependent vasorelaxation, with most significant effect induced by aqueous fraction (AS-CS). This vasorelaxant effect of AS-CS was reduced by inhibition of nitric oxide pathways and inhibition of potassium channels. Inhibition of phosphatidylinositol 3- kinase and Src tyrosine kinase, as well as scavenging of reactive oxygen species, produced an attenuation of the relaxation response. Estrogen receptor antagonists (tamoxifen and ICI 182,782) reduced the AS-CS mediated vasorelaxation. AS-CS also stimulated the endothelial formation of ROS and phosphorylation of Akt and eNOS. CONCLUSION: The data indicated that C. songarica produces an endothelium-dependent vasorelaxation, which is partly dependent upon estrogen receptors, and sensitive to inhibition of ROS/Src/PI3K/NO pathways.

scholarly journals The role of reactive oxygen species in the infarct-limiting effect of hypoxic preconditioning

2021 ◽  
Vol 20 (2) ◽  
pp. 87-91
Author(s):  
A. S. Sementsov ◽  
N. V. Naryzhnaya ◽  
M. A. Sirotina ◽  
L. N. Maslov
Keyword(s):  
Reactive Oxygen Species ◽  
Coronary Artery ◽  
Ischemia Reperfusion ◽  
Reactive Oxygen ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Hypoxic Preconditioning ◽  
Male Rats ◽  
Oxygen Species ◽  
Infarct Size Reduction

Introduction. Increased resistance of the heart to ischemia/reperfusion (I/R) is an urgent aim of physiology, pharmacology, and cardiac surgery, since I/R injury of the heart is often the cause of cardiogenic shock and subsequent death of patients in the postoperative period. Materials and methods. The study was carried out in male rats which were subjected to coronary artery occlusion (45 min) and reperfusion (2 h). Before coronary occlusion, early hypoxic preconditioning (HP) was modeled. The rats were subjected to six sessions of hypoxia (8 % O2, 10 min) and reoxygenation (21 % O2, 10 min) 30 min before coronary artery occlusion. The rats were injected with the following drugs: 1,3-dimethylthiourea (DMTM), 2-mercaptopropionyl glycine (2-MPG), deferoxamine. Results. It was found that HP contributes to infarct size reduction by 30 %. Preliminary administration of DMTM, 2-MPG, deferoxamine eliminated the infarct-reducing effect of HP. Conclisuon. The obtained data indicate that reactive oxygen species are involved in the cardioprotective effect of HP.

Reactive oxygen species are critical mediators of coronary collateral development in a canine model

2003 ◽  
Vol 285 (4) ◽  
pp. H1582-H1589 ◽  
Author(s):  
Weidong Gu ◽  
Dorothee Weihrauch ◽  
Katsuya Tanaka ◽  
John P. Tessmer ◽  
Paul S. Pagel ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Blood Flow ◽  
Coronary Artery ◽  
Reactive Oxygen ◽  
Coronary Artery Occlusion ◽  
Artery Occlusion ◽  
Oxygen Species ◽  
Collateral Blood Flow ◽  
Collateral Development ◽  
Coronary Collateral

Recent evidence suggests that reactive oxygen species (ROS) promote proliferation and migration of vascular smooth muscle (VSMC) and endothelial cells (EC). We tested the hypothesis that ROS serve as crucial messengers during coronary collateral development. Dogs were subjected to brief (2 min), repetitive coronary artery occlusions (1/h, 8/day, 21 day duration) in the absence (occlusion, n = 8) or presence of N-acetylcysteine (NAC) (occlusion + NAC, n = 8). A sham group ( n = 8) was instrumented identically but received no occlusions. In separate experiments, ROS generation after a single 2-min coronary artery occlusion was assessed with dihydroethidium fluorescence. Coronary collateral blood flow (expressed as a percentage of normal zone flow) was significantly increased (71 ± 7%) in occlusion dogs after 21 days but remained unchanged (13 ± 3%) in sham dogs. Treatment with NAC attenuated increases in collateral blood flow (28 ± 8%). Brief coronary artery occlusion and reperfusion caused ROS production (256 ± 33% of baseline values), which was abolished with NAC (104 ± 12%). Myocardial interstitial fluid produced tube formation and proliferation of VSMC and EC in occlusion but not in NAC-treated or sham dogs. The results indicate that ROS are critical for the development of the coronary collateral circulation.

scholarly journals Reactive Oxygen Species Modulator-1 (ROMO-1) Polymorphism Rs6060566 and The Risk of Myocardial Infarction in Slovenian Subjects With Type 2 Diabetes Mellitus

2020 ◽  
Author(s):  
Miha Tibaut ◽  
Sara Mankoč Ramuš ◽  
Daniel Petrovič
Keyword(s):  
Diabetes Mellitus ◽  
Myocardial Infarction ◽  
Reactive Oxygen Species ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Coronary Artery ◽  
Coronary Arteries ◽  
Reactive Oxygen ◽  
Oxygen Species

Abstract Background We aimed to examine the role of the rs6060566 polymorphism of the reactive oxygen species modulator-1 (ROMO-1) gene in the development of myocardial infarction (MI) in Caucasians with type 2 diabetes mellitus (T2DM). Methods A total of 1072 subjects with T2DM were enrolled in cross-sectional case-control study: 335 subjects with MI and 737 subjects without clinical signs of coronary artery disease (CAD). Genetic analysis of the rs6060566 polymorphism was performed in all subjects. To assess the degree of coronary artery obstruction, a subpopulation of 128 subjects with T2DM underwent coronary computed tomography (CT) angiography. Next, endarterectomy samples were obtained during myocardial revascularization from diffusely diseased coronary arteries in 40 cases, which were analysed for ROMO-1 expression according to their genotype. Results There were no statistically significant associations between different genotypes or alleles of the rs6060566 polymorphism and MI in subjects with T2DM. The carriers of the C allele of the ROMO-1 rs6060566 had a threefold increased likelihood of having coronary artery stenosis (AOR = 3.27, 95% CI 1.16–9.20). Furthermore, the carriers of the C allele showed higher number of positive cells for ROMO-1 expression in endarterectomy samples of coronary arteries. Conclusions In accordance to our study, the rs6060566 polymorphism of the ROMO-1 gene is not the risk factor for MI in Caucasians with T2DM. However, we found that subjects carrying the C allele were at a 3.27-fold increased risk of developing severe CAD compared with those who had nonobstructive CAD. Moreover, The C allele carriers showed statistically higher number of cells positive for ROMO-1 compared with T allele carriers in coronary endarterectomy samples.

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