High-Performance Liquid Chromatographic Mapping of Oligosaccharides Using High pH Anion-Exchange Chromatography: Improvements from Sample Preparation, Reduction, and Fluorometric Detection

1997 ◽  
pp. 467-480
Keyword(s):  
Sample Preparation ◽  
Anion Exchange ◽  
High Performance ◽  
Anion Exchange Chromatography ◽  
High Performance Liquid Chromatographic ◽  
Exchange Chromatography ◽  
Fluorometric Detection ◽  
High Ph ◽  
Liquid Chromatographic

scholarly journals High-Performance Anion-Exchange Chromatography Coupled with Pulsed Electrochemical Detection as a Powerful Tool to Evaluate Carbohydrates of Food Interest: Principles and Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-13 ◽  
Author(s):  
Claudio Corradini ◽  
Antonella Cavazza ◽  
Chiara Bignardi
Keyword(s):  
Anion Exchange ◽  
Electrochemical Detection ◽  
High Performance ◽  
Oxidation Mechanism ◽  
Amperometric Detection ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
High Ph ◽  
Analytical Technique ◽  
Pulsed Electrochemical Detection

Specific HPLC approaches are essential for carbohydrate characterization in food products. Carbohydrates are weak acids with pKa values in the range 12–14 and, consequently, at high pH can be transformed into oxyanions, and can be readily separated using highly efficient anion-exchange columns. Electrochemical detection in HPLC has been proven to be a powerful analytical technique for the determination of compounds containing electroactive groups; pulsed amperometric detection of carbohydrates is favourably performed by taking advantage of their electrocatalytic oxidation mechanism at a gold working electrode in a basic media. High-performance Anion Exchange Chromatography (HPAEC) at high pH coupled with pulsed electrochemical detection (PED) is one of the most useful techniques for carbohydrate determination either for routine monitoring or research application. This technique has been of a great impact on the analysis of oligo- and polysaccharides. The compatibility of electrochemical detection with gradient elution, coupled with the high selectivity of the anion-exchange stationary phases, allows mixtures of simple sugars, oligo- and polysaccharides to be separated with high resolution in a single run. A few reviews have been written on HPAEC-PED of carbohydrates of food interest in the last years. In this paper the recent developments in this field are examined.

Simultaneous liquid-chromatographic analysis for 4-hydroxy-3-methoxymandelic acid and 4-hydroxy-3-methoxyphenylacetic acid in urine.

1980 ◽  
Vol 26 (2) ◽  
pp. 291-294
Author(s):  
S J Soldin ◽  
J G Hill
Keyword(s):  
High Performance ◽  
Potassium Phosphate ◽  
Amperometric Detection ◽  
Anion Exchange Chromatography ◽  
High Performance Liquid Chromatographic ◽  
Exchange Chromatography ◽  
Liquid Chromatograph ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract We describe a rapid, reliable “high-performance" liquid-chromatographic method of simultaneously analyzing for 4-hydroxy-3-methoxymandelic acid (I) and 4-hydroxy-3-methoxyphenylacetic acid (II) in urine. Paired-ion chromatography and amperometric detection are used in the method. A 5-mL aliquot of urine is adjusted to pH 6.1 and subjected to anion-exchange chromatography (Dowex AG 1-X4, 1.8 X 0.7 cm column). The two analytes are eluted from the column with 10 mL of 3 mol/L sodium chloride, and 2 microL of the eluate, injected directly into the liquid chromatograph, is chromatographed at 40 degrees C. This column contains mu Bondapak C18, and the mobile phase is 100 mmol/L potassium phosphate buffer, pH 6.7, containing 0.5 mmol of tetrabutylammonium phosphate per liter. Detector sensitivity is 50 nA full scale. Analytical recoveries of I and II are 80--84% and 48--49%, respectively; the coefficients of variation for various concentrations in urine are about 10%.

scholarly journals Simultaneous liquid-chromatographic analysis for 4-hydroxy-3-methoxymandelic acid and 4-hydroxy-3-methoxyphenylacetic acid in urine.

1980 ◽  
Vol 26 (2) ◽  
pp. 291-294 ◽  
Author(s):  
S J Soldin ◽  
J G Hill
Keyword(s):  
High Performance ◽  
Potassium Phosphate ◽  
Amperometric Detection ◽  
Anion Exchange Chromatography ◽  
High Performance Liquid Chromatographic ◽  
Exchange Chromatography ◽  
Liquid Chromatograph ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract We describe a rapid, reliable “high-performance" liquid-chromatographic method of simultaneously analyzing for 4-hydroxy-3-methoxymandelic acid (I) and 4-hydroxy-3-methoxyphenylacetic acid (II) in urine. Paired-ion chromatography and amperometric detection are used in the method. A 5-mL aliquot of urine is adjusted to pH 6.1 and subjected to anion-exchange chromatography (Dowex AG 1-X4, 1.8 X 0.7 cm column). The two analytes are eluted from the column with 10 mL of 3 mol/L sodium chloride, and 2 microL of the eluate, injected directly into the liquid chromatograph, is chromatographed at 40 degrees C. This column contains mu Bondapak C18, and the mobile phase is 100 mmol/L potassium phosphate buffer, pH 6.7, containing 0.5 mmol of tetrabutylammonium phosphate per liter. Detector sensitivity is 50 nA full scale. Analytical recoveries of I and II are 80--84% and 48--49%, respectively; the coefficients of variation for various concentrations in urine are about 10%.

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