scholarly journals PLASMA MEMBRANE VESICLES FROM TYPE II PNEUMOCYTES INSERT CATION CHANNELS INTO PLANAR LIPID BILAYERS. 2319

1996 ◽  
Vol 39 ◽  
pp. 389-389
Author(s):  
David G Oelberg ◽  
Ana Raiko ◽  
Fang Xu ◽  
Dieter K Bartschat
Keyword(s):  
Plasma Membrane ◽  
Lipid Bilayers ◽  
Membrane Vesicles ◽  
Cation Channels ◽  
Planar Lipid Bilayers ◽  
Type Ii ◽  
Plasma Membrane Vesicles ◽  
Type Ii Pneumocytes

Anion channels in rat liver canalicular plasma membranes reconstituted into planar lipid bilayers

1992 ◽  
Vol 262 (6) ◽  
pp. G1027-G1032 ◽  
Author(s):  
M. Sellinger ◽  
S. A. Weinman ◽  
R. M. Henderson ◽  
A. Zweifach ◽  
J. L. Boyer ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Lipid Bilayers ◽  
Rat Liver ◽  
Membrane Vesicles ◽  
Planar Lipid Bilayers ◽  
Anion Channels ◽  
Plasma Membrane Vesicles ◽  
Patch Clamp Technique ◽  
Bile Formation ◽  
Cl Conductance

Previous studies from this laboratory have demonstrated a Cl(-)-HCO3- exchanger and have provided evidence for a Cl- conductance in rat liver canalicular plasma membrane vesicles. To further investigate the apical Cl- conductance, we performed single-channel analysis after incorporation of canalicular liver plasma membrane vesicles into planar lipid bilayers. This was necessary, because the canalicular membrane is not accessible for the patch-clamp technique. Two types of anion channels could be identified (30- and 90-pS conductance) corresponding to the class of small and intermediate channels, respectively. The kinetics of the small channel were found to be voltage dependent with a maximum for the open probability at -20 mV. In contrast, intermediate channel kinetics were voltage independent. The anion channels described above could allow electrogenic Cl- efflux, to compensate Cl- influx via the electroneutral Cl(-)-HCO3- exchanger. Further studies will be required to prove their functional importance in bile formation.

scholarly journals Batrachotoxin-activated Na+ channels in planar lipid bilayers. Competition of tetrodotoxin block by Na+.

1984 ◽  
Vol 84 (5) ◽  
pp. 665-686 ◽  
Author(s):  
E Moczydlowski ◽  
S S Garber ◽  
C Miller
Keyword(s):  
Lipid Bilayers ◽  
Membrane Vesicles ◽  
Receptor Site ◽  
Single Channels ◽  
Planar Lipid Bilayers ◽  
Na Channels ◽  
Plasma Membrane Vesicles ◽  
Voltage Range ◽  
Conduction Pathway ◽  
Almost All

Single Na+ channels from rat skeletal muscle plasma membrane vesicles were inserted into planar lipid bilayers formed from neutral phospholipids and were observed in the presence of batrachotoxin. The batrachotoxin-modified channel activates in the voltage range -120 to -80 mV and remains open almost all the time at voltages positive to -60 mV. Low levels of tetrodotoxin (TTX) induce slow fluctuations of channel current, which represent the binding and dissociation of single TTX molecules to single channels. The rates of association and dissociation of TTX are both voltage dependent, and the association rate is competitively inhibited by Na+. This inhibition is observed only when Na+ is increased on the TTX binding side of the channel. The results suggest that the TTX receptor site is located at the channel's outer mouth, and that the Na+ competition site is not located deeply within the channel's conduction pathway.

Calcium efflux of plasma membrane vesicles exposed to ELF magnetic fields-test of a nuclear magnetic resonance interaction model

2012 ◽  
Vol 33 (7) ◽  
pp. 535-542 ◽  
Author(s):  
Wenjun J. Sun ◽  
Mehri Kaviani Mogadam ◽  
Marianne Sommarin ◽  
Henrietta Nittby ◽  
Leif G. Salford ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Plasma Membrane ◽  
Magnetic Resonance ◽  
Magnetic Fields ◽  
Interaction Model ◽  
Membrane Vesicles ◽  
Resonance Interaction ◽  
Calcium Efflux ◽  
Plasma Membrane Vesicles ◽  
Nuclear Magnetic
Sign in / Sign up

Export Citation Format

Share Document