scholarly journals Neural retina limits the nonviral gene transfer to retinal pigment epithelium in an in vitro bovine eye model

2004 ◽  
Vol 6 (3) ◽  
pp. 72-80 ◽  
Author(s):  
Leena Pitkänen ◽  
Jukka Pelkonen ◽  
Marika Ruponen ◽  
Seppo Rönkkö ◽  
Arto Urtti
Keyword(s):  
Retinal Pigment Epithelium ◽  
Gene Transfer ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Neural Retina ◽  
Nonviral Gene Transfer ◽  
Eye Model

scholarly journals Comparative Analysis of Gene Transfer to Human and Rat Retinal Pigment Epithelium Cell Line by a Combinatorial Use of Recombinant Adeno- Associated Virus and Ultrasound or/and Microbubbles

2009 ◽  
Vol 9 (3) ◽  
pp. 174-181 ◽  
Author(s):  
Xiao-Zhi Zheng ◽  
Hong-Li Li ◽  
Lian-Fang Du ◽  
Hui-Ping Wang ◽  
Qing Gu
Keyword(s):  
Retinal Pigment Epithelium ◽  
Gene Transfer ◽  
Cell Line ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelium Cell ◽  
Blue Staining ◽  
Adeno Associated Virus ◽  
Epithelium Cell

Ultrasound-targeted microbubble destruction has been utilized to deliver a drug/gene into cells in both in vitro and in vivo studies. This work was performed to investigate the feasibility of gene transfer to human retinal pigment epithelium cell line(ARPE-19) and rat retinal pigment epithelium cell line(RPE-J) by a combinatorial use of recombinant adeno-associated virus (rAAV) and ultrasound (US) or/and mi-crobubbles (MBs) and compare the difference between them. Different doses of serotype 2 rAAV encoding a enhanced green fluorescent protein (rAAV2-EGFP) gene and MBs was administered to ARPE-19 and RPE-J cells under different US conditions. Transfection efficiency and cell viability were assessed by fluorescence microscopy, flow cytometry (FCM) analysis, trypan blue staining. The results indicated that US and MBs could respectively improve rAAV2mediated gene transfer to RPE-J cells, but neither US nor MBs could do so in ARPE- 19 cells. US plus MBs could significantly enhance rAAV2-mediated gene transfer to ARPE-19 cells, however, the same effects were not seen in RPE-J cells. These findings demonstrated it is not always coincident that US, MBs and US plus MBs exert the similar effects on gene transfer in vitro RPE cells. So, it is necessary to choose appropriate RPE cell line for the study of US or/and MBs-mediated rAAV gene transfer in retinal gene therapy.

In vitro transdifferentiation of embryonic rat retinal pigment epithelium to neural retina

1995 ◽  
Vol 677 (2) ◽  
pp. 300-310 ◽  
Author(s):  
Shulei Zhao ◽  
Steven C. Thornquist ◽  
Colin J. Barnstable
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Neural Retina

Production of interleukin-6 by human retinal pigment epithelium in vitro and its regulation by other cytokines

1992 ◽  
Vol 11 (sup1) ◽  
pp. 173-179 ◽  
Author(s):  
Mark T. Benson ◽  
Lynda Shepherd ◽  
Robert C. Rees ◽  
Ian G. Rennie
Keyword(s):  
Retinal Pigment Epithelium ◽  
Interleukin 6 ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Human Retinal Pigment Epithelium

Maintenance of adult porcine retina and retinal pigment epithelium in perfusion culture: Characterisation of an organotypic in vitro model

2008 ◽  
Vol 86 (4) ◽  
pp. 661-668 ◽  
Author(s):  
Karin Kobuch ◽  
Wolfgang A. Herrmann ◽  
Carsten Framme ◽  
Helmut G. Sachs ◽  
Veit-Peter Gabel ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
In Vitro Model ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Perfusion Culture ◽  
Vitro Model

The cytoskeletal elements of human retinal pigment epithelium: in vitro and in vivo

1988 ◽  
Vol 91 (2) ◽  
pp. 303-312
Author(s):  
N.M. McKechnie ◽  
M. Boulton ◽  
H.L. Robey ◽  
F.J. Savage ◽  
I. Grierson
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Cytokeratin 18 ◽  
Rpe Cells ◽  
Human Retinal Pigment Epithelium ◽  
Cytoskeletal Elements

The cytoskeletal elements of normal (in situ) and cultured human retinal pigment epithelium (RPE) were studied by a variety of immunocytochemical techniques. Primary antibodies to vimentin and cytokeratins were used. Positive immunoreactivity for vimentin was obtained with in situ and cultured material. The pattern of reactivity obtained with antisera and monoclonals to cytokeratins was more complex. Cytokeratin immunoreactivity could be demonstrated in situ and in cultured cells. The pattern of cytokeratin expression was similar to that of simple or glandular epithelia. A monoclonal antibody that specifically recognizes cytokeratin 18 identified a population of cultured RPE cells that had particularly well-defined filamentous networks within their cytoplasm. Freshly isolated RPE was cytokeratin 18 negative by immunofluorescence, but upon culture cytokeratin 18 positive cells were identifiable. Cytokeratin 18 positive cells were identified in all RPE cultures (other than early primaries), regardless of passage number, age or sex of the donor. In post-confluent cultures cytokeratin 18 cells were identified growing over cytokeratin 18 negative cells, suggesting an association of cytokeratin 18 immunoreactivity with cell proliferation. Immunofluorescence studies of retinal scar tissue from two individuals revealed the presence of numerous cytokeratin 18 positive cells. These findings indicate that RPE cells can be identified by their cytokeratin immunoreactivity and that the overt expression of cytokeratin 18 may be associated with proliferation of human RPE both in vitro and in vivo.

Coexpression of FGF-5 and bFGF by the retinal pigment epithelium in vitro

1992 ◽  
Vol 55 (5) ◽  
pp. 727-734 ◽  
Author(s):  
Laurie M. Bost ◽  
Amy E. Aotaki-Keen ◽  
Leonard M. Hjelmeland
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment
Sign in / Sign up

Export Citation Format

Share Document