Effects of Starvation in Rats on Serum Levels of Follicle Stimulating Hormone, Luteinizing Hormone, Thyrotropin, Growth Hormone and Prolactin; Response to LH-Releasing Hormone and Thyrotropin-Releasing Hormone

Endocrinology ◽  
1977 ◽  
Vol 100 (2) ◽  
pp. 580-587 ◽  
Author(s):  
G. A. CAMPBELL ◽  
M. KURCZ ◽  
S. MARSHALL ◽  
J. MEITES
Keyword(s):  
Growth Hormone ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Serum Levels ◽  
Thyrotropin Releasing Hormone ◽  
Releasing Hormone ◽  
Prolactin Response ◽  
Lh Releasing Hormone ◽  
Stimulating Hormone

EFFECT OF SYNTHETIC LUTEINIZING HORMONE-AND FOLLICLE STIMULATING HORMONE-RELEASING HORMONE (LH/FSH-RH) ON SERUM LEVELS OF LH, FSH, THYROTROPHIN (TSH) AND GROWTH HORMONE (HGH) IN NORMAL MALE SUBJECTS

1973 ◽  
Vol 73 (3) ◽  
pp. 465-474 ◽  
Author(s):  
Egil Haug ◽  
Peter Torjesen
Keyword(s):  
Growth Hormone ◽  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Normal Male ◽  
Serum Growth Hormone ◽  
Maximum Serum ◽  
Releasing Hormone ◽  
Serum Lh ◽  
Male Subjects ◽  
Stimulating Hormone

ABSTRACT Four normal male subjects received iv injections of synthetic luteinizing hormone- and follicle stimulating hormone-releasing hormone (LH/FSH-RH) in doses of 12.5, 25, 100, 200 and 400 μg, respectively. A dose of 12.5 μg of LH/FSH-RH caused a significant increase in serum FSH, and 25 μg significantly increased the serum LH. The peak responses occurred 15 to 30 min after the LH/FSH-RH injections in most of the experiments. The increase in the mean maximum serum LH and FSH levels was 2 to 4 fold. There was great variation in response between the subjects, but when tested repeatedly with the same dose of LH/FSH-RH a given individual responded in a consistent manner. The log dose-response curve between LH/FSH-RH and serum LH, and between LH/FSH-RH and serum FSH was approximately linear. A small but significant (P < 0.05) rise in serum thyrotrophin (TSH) was found after LH/FSH-RH in doses ranging from 25 to 400 μg. There was no significant rise in serum growth hormone (HGH). On the basis of the present study a standard 100 μg iv LH/FSH-RH test is suggested.

DEVELOPMENT AND APPLICATION OF HOMOLOGOUS RADIOIMMUNOASSAYS FOR PORCINE GONADOTROPHINS

1979 ◽  
Vol 81 (1) ◽  
pp. 1-10 ◽  
Author(s):  
J. L. VANDALEM ◽  
CH. BODART ◽  
G. PIRENS ◽  
J. CLOSSET ◽  
G. HENNEN
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Oestrous Cycle ◽  
Releasing Hormone ◽  
Porcine Serum ◽  
Lh Releasing Hormone ◽  
Stimulating Hormone

Antisera were raised against highly purified preparations of porcine luteinizing hormone (pLH) and follicle-stimulating hormone (pFSH). Highly specific and sensitive radioimmunoassay systems were developed. The antisera to LH and FSH were used at working dilutions of 1: 500 000 and 1: 200 000 respectively and the sensitivities of the assays were 0·1 ng LH/ml serum (3 × 10−12 mol/l) and 0·5 ng FSH/ml serum (1·5 × 10−11 mol/l). The LH and FSH preparations used as standards were 1·2 and 81 times as potent as NIH-LH-S15 and NIH-FSH-P1 respectively. Both assays were validated and adapted for the measurement of the gonadotrophin content of porcine serum. The concentrations of LH and FSH in blood were measured simultaneously in prepubertal sows throughout a 24 h period, in adult sows during the oestrous cycle and in both prepubertal and adult animals after treatment with LH releasing hormone.

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