Measurement of Serum LH, FSH, Estradiol and Progesterone in Disorders of the Human Menstrual Cycle: The Inadequate Luteal Phase

Plasma bradykininogen levels have been shown to rise about threefold late in pregnancy in the rat. However, they declined sharply 1–2 days before delivery.Plasma bradykininogen levels remained relatively unchanged through most the oestrous and menstrual cycles (rat, man). However, they showed a fall at two similar periods, in both cycles. A smaller decline occurred in metoestrus in the rat, and (probably) in the late luteal phase in the human. The largest fall took place around the time of ovulation in both the oestrous and the menstrual cycles. The decline was about 59% in the rat and 42% in the human. Bradykininogen showed no similar changes in the blood of male human controls. The suggestion that bradykinin could be involved in ovulation is discussed.

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MEASUREMENT OF PLASMA LH, FSH, ESTRADIOL AND PROGESTERONE IN DISORDERS OF THE HUMAN MENSTRUAL CYCLE: THE SHORT LUTEAL PHASE

Obstetrical & Gynecological Survey ◽

10.1097/00006254-197411000-00020 ◽

1974 ◽

Vol 29 (11) ◽

pp. 820-822 ◽

Cited By ~ 1

Author(s):

BARRY M. SHERMAN ◽

STANLEY G. KORENMAN

Keyword(s):

Menstrual Cycle ◽

Luteal Phase ◽

Human Menstrual Cycle

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Homologous in vitro bioassay for follicle-stimulating hormone (FSH) reveals increased FSH biological signal during the mid- to late luteal phase of the human menstrual cycle

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.81.6.2080 ◽

1996 ◽

Vol 81 (6) ◽

pp. 2080-2088 ◽

Cited By ~ 9

Author(s):

S. Christin-Maitre

Keyword(s):

Menstrual Cycle ◽

Luteal Phase ◽

Follicle Stimulating Hormone ◽

In Vitro Bioassay ◽

Biological Signal ◽

Late Luteal Phase ◽

Human Menstrual Cycle ◽

Stimulating Hormone

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CHANGES IN THE BINDING OF HUMAN CHORIONIC GONADOTROPHIN/LUTEINIZING HORMONE, FOLLICLE-STIMULATING HORMONE AND PROLACTIN TO HUMAN CORPORA LUTEA DURING THE MENSTRUAL CYCLE AND PREGNANCY

Journal of Endocrinology ◽

10.1677/joe.0.0870315 ◽

1980 ◽

Vol 87 (3) ◽

pp. 315-325 ◽

Cited By ~ 28

Author(s):

A. S. McNEILLY ◽

J. KERIN ◽

I. A. SWANSTON ◽

T. A. BRAMLEY ◽

D. T. BAIRD

Keyword(s):

Menstrual Cycle ◽

Luteinizing Hormone ◽

Luteal Phase ◽

Follicle Stimulating Hormone ◽

Chorionic Gonadotrophin ◽

Human Chorionic Gonadotrophin ◽

Corpora Lutea ◽

Maximum Serum ◽

Human Menstrual Cycle ◽

Stimulating Hormone

The changes in the binding of human chorionic gonadotrophin/luteinizing hormone (HCG/LH), follicle-stimulating hormone (FSH) and prolactin to 44 corpora lutea have been assessed during the luteal phase of the human menstrual cycle and early pregnancy. All corpora lutea bound HCG but out of 32 only ten bound FSH and only seven bound prolactin specifically. While binding of HCG increased to maximal levels in the mid-luteal phase, binding of FSH and prolactin was most often found in the early luteal phase. Maximum binding of HCG was associated with maximum serum levels of progesterone. Luteal regression was associated with a decrease in the binding of HCG but a causal relationship could not be established. Very low binding of HCG was found to corpora lutea of pregnancy. These results show that (1) the changes in binding of HCG during the luteal phase of the human menstrual cycle are similar to those in other species and (2) there are specific binding sites for prolactin and FSH in the human corpus luteum.

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PERIPHERAL PLASMA LEVELS OF 17α-HYDROXYPROGESTERONE, PROGESTERONE AND OESTRADIOL DURING NORMAL MENSTRUAL CYCLES IN WOMEN

Acta Endocrinologica ◽

10.1530/acta.0.0710743 ◽

1972 ◽

Vol 71 (4) ◽

pp. 743-754 ◽

Cited By ~ 10

Author(s):

Tore H:son Holmdahl ◽

Elof D. B. Johansson

Keyword(s):

Menstrual Cycle ◽

Plasma Levels ◽

Luteal Phase ◽

Cycle Length ◽

Gel Chromatography ◽

Blood Samples ◽

Menstrual Cycles ◽

Peripheral Plasma ◽

Competitive Protein Binding ◽

Human Menstrual Cycle

ABSTRACT Liquid-gel chromatography on hydroxyalkoxypropyl Sephadex has been used to separate 17α-hydroxyprogesterone* and progesterone from interfering steroids prior to assay by competitive protein binding. During the luteal phase 0.5 ml of plasma was enough for determinations of both steroids. Fifteen samples could be assayed in less than 48 h. Oestradiol in plasma was assayed by radioimmunoassay. Daily blood samples were collected during 10 normal menstrual cycles in young, healthy women. The average cycle length was 29.7±2.0 (sd) days. 17α-hydroxyprogesterone displayed a midcyclic peak averaging 1.86±0.70 (sd) ng per ml coinciding with a midcyclic peak of oestradiol averaging 16.50± 5.95 (sd) ng per 100 ml of plasma. The highest luteal phase level of 17α-hydroxyprogesterone was 1.94 ±0.72 (sd) ng per ml. The corresponding levels for oestradiol were 9.1 ±3.8 (sd) ng per 100 ml. Progesterone formed a luteal plateau averaging 12.3±2.3 (sd) days. The highest luteal level of progesterone was 14.6±2.1 (sd) ng per ml. The peripheral plasma pattern of 17α-hydroxyprogesterone seems to mimic that of oestradiol during the human menstrual cycle.

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