Liver X Receptor-α Regulates Proopiomelanocortin (POMC) Gene Transcription in the Pituitary

Abstract Lipopolysaccharides (LPS) could induce milk fat depression via regulating the body and blood fat metabolism. However, it is not completely clear how LPS might regulate triglyceride synthesis in dairy cow mammary epithelial cells (DCMECs). DCMECs were isolated and purified from dairy cow mammary tissue and treated with LPS. The level of triglyceride synthesis, the expression and activity of the liver X receptor α (LXRα), enzymes related to de novo fatty acid synthesis, and the expression of the fatty acid transporters were investigated. We found that LPS decreased the level of triglyceride synthesis via a down-regulation of the transcription, translation, and nuclear translocation level of the LXRα. The results also indicated that the transcription level of the LXRα target genes, sterol regulatory element binding protein 1 (SREBP1), fatty acid synthetase (FAS), acetyl-CoA carboxylase-1 (ACC1), were significantly down-regulated in DCMECs after LPS treatment. Our data may provide new insight into the mechanisms of milk fat depression caused by LPS.

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Interleukin-10 increases reverse cholesterol transport in macrophages through its bidirectional interaction with liver X receptor α

Biochemical and Biophysical Research Communications ◽

10.1016/j.bbrc.2014.07.036 ◽

2014 ◽

Vol 450 (4) ◽

pp. 1525-1530 ◽

Cited By ~ 6

Author(s):

Bente Halvorsen ◽

Sverre Holm ◽

Arne Yndestad ◽

Hanne Scholz ◽

Ellen Lund Sagen ◽

...

Keyword(s):

Reverse Cholesterol Transport ◽

Interleukin 10 ◽

Liver X Receptor ◽

Cholesterol Transport ◽

Liver X Receptor Α

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Immunohistochemical Expression of Cyclo-oxygenase 2 and Liver X Receptor-α in Acne Vulgaris

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2017/28754.10577 ◽

2017 ◽

Author(s):

Ola Ahmed Bakry

Keyword(s):

Acne Vulgaris ◽

Liver X Receptor ◽

Immunohistochemical Expression ◽

Liver X Receptor Α

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Dichlorodiphenyltrichloroethane Impairs Amyloid Beta Clearance by Decreasing Liver X Receptor α Expression

Frontiers in Aging Neuroscience ◽

10.3389/fnagi.2021.634948 ◽

2021 ◽

Vol 13 ◽

Author(s):

Dongmei Wu ◽

Yang Hu ◽

Min Song ◽

Gongbo Li

Keyword(s):

Amyloid Beta ◽

Critical Role ◽

Liver X Receptor ◽

Dynamics Simulation ◽

Atp Binding ◽

Atp Binding Cassette Transporter ◽

Liver X Receptor Α ◽

Aβ Clearance ◽

Atp Binding Cassette

Abnormal amyloid beta (Aβ) clearance is a distinctive pathological mechanism for Alzheimer’s disease (AD). ATP-binding cassette transporter A1 (ABCA1), which mediates the lipidation of apolipoprotein E, plays a critical role in Aβ clearance. As an environmental factor for AD, dichlorodiphenyltrichloroethane (DDT) can decrease ATP-binding cassette transporter A1 (ABCA1) expression and disrupt Aβ clearance. Liver X receptor α (LXRα) is an autoregulatory transcription factor for ABCA1 and a target of some environmental pollutants, such as organophosphate pesticides. In this study, we aimed to investigate whether DDT could affect Aβ clearance by targeting LXRα. The DDT-pretreated H4 human neuroglioma cells and immortalized astrocytes were incubated with exogenous Aβ to evaluate Aβ consumption. Meanwhile, cytotoxicity and LXRα expression were determined in the DDT-treated cells. Subsequently, the antagonism of DDT on LXRα agonist T0901317 was determined in vitro. The interaction between DDT and LXRα was predicted by molecular docking and molecular dynamics simulation technology. We observed that DDT could inhibit Aβ clearance and decrease the levels of LXRα mRNA and LXRα protein. Moreover, DDT is supposed to strongly bind to LXRα and exert antagonistic effects on LXRα. In conclusion, this study firstly presented that DDT could inhibit LXRα expression, which would contribute to Aβ clearance decline in vitro. It provides an experimental basis to search for potential therapeutic targets of AD.

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