Goat liver X receptor α, molecular cloning, functional characterization and regulating fatty acid synthesis in epithelial cells of goat mammary glands

Gene ◽  
2012 ◽  
Vol 505 (1) ◽  
pp. 114-120 ◽  
Author(s):  
Wei Wang ◽  
Jun Luo ◽  
Yu Zhong ◽  
Xian-Zi Lin ◽  
Heng-Bo Shi ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Molecular Cloning ◽  
Fatty Acid Synthesis ◽  
Functional Characterization ◽  
Acid Synthesis ◽  
Mammary Glands ◽  
Liver X Receptor ◽  
Liver X Receptor Α

Fatty acid synthesis by explant cultures from the mammary glands of goats on days 60 and 120 of pregnancy

1984 ◽  
Vol 100 (1) ◽  
pp. 87-NP ◽  
Author(s):  
I. A. Forsyth ◽  
A. Turvey
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Fatty Acid Synthesis ◽  
Milk Fat ◽  
Secretory Activity ◽  
Acid Synthesis ◽  
Mammary Glands ◽  
Mammary Tissue ◽  
Histological Structure ◽  
Fourfold Increase

ABSTRACT Explants of mammary glands from 60-day pregnant goats showed a mean fourfold increase in fatty acid synthesis from acetate when cultured with insulin+ cortisol. Epithelial cells increased their area by 60% but no secretory activity was induced. In 120-day pregnant goats, fatty acid synthesis and epithelial cell area were greater than at day 60 of pregnancy and were unaffected by hypophysectomy or by daily treatment with bromocriptine from day 60. Neither increased further on culture of mammary explants in insulin + cortisol. Ovine prolactin increased fatty acid synthesis two-fold when added to insulin + cortisol in cultures of mammary tissue from goats on day 60 of pregnancy and secretory activity was induced. On day 120 of pregnancy insulin + cortisol + prolactin sustained or slightly stimulated both fatty acid synthesis and the extensive secretion present in the tissue at the start of culture. Synthesis of medium-chain fatty acids of milk-fat was also sustained by prolactin in one goat. An atmosphere of air was found to maintain normal histological structure of the mammary gland. By contrast, in 95% oxygen, explants from goats which were 60 days pregnant showed epithelial cells filling the lumina of ducts and alveoli in 60% of explants and a poor response to prolactin. J. Endocr. (1984) 100, 87–92

Liver X receptor α participates in LPS-induced reduction of triglyceride synthesis in bovine mammary epithelial cells

2020 ◽  
pp. 1-7
Author(s):  
Jianfa Wang ◽  
Shuai Lian ◽  
Jun Song ◽  
Hai Wang ◽  
Xu Zhang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Dairy Cow ◽  
Milk Fat ◽  
Mammary Epithelial Cells ◽  
Liver X Receptor ◽  
Mammary Epithelial ◽  
Triglyceride Synthesis ◽  
Milk Fat Depression ◽  
Liver X Receptor Α

Abstract Lipopolysaccharides (LPS) could induce milk fat depression via regulating the body and blood fat metabolism. However, it is not completely clear how LPS might regulate triglyceride synthesis in dairy cow mammary epithelial cells (DCMECs). DCMECs were isolated and purified from dairy cow mammary tissue and treated with LPS. The level of triglyceride synthesis, the expression and activity of the liver X receptor α (LXRα), enzymes related to de novo fatty acid synthesis, and the expression of the fatty acid transporters were investigated. We found that LPS decreased the level of triglyceride synthesis via a down-regulation of the transcription, translation, and nuclear translocation level of the LXRα. The results also indicated that the transcription level of the LXRα target genes, sterol regulatory element binding protein 1 (SREBP1), fatty acid synthetase (FAS), acetyl-CoA carboxylase-1 (ACC1), were significantly down-regulated in DCMECs after LPS treatment. Our data may provide new insight into the mechanisms of milk fat depression caused by LPS.

scholarly journals Initiation of fatty acid synthesis in rat mammary glands

1980 ◽  
Vol 190 (1) ◽  
pp. 171-175 ◽  
Author(s):  
P Martyn ◽  
I A Hansen
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Post Partum ◽  
Acid Synthesis ◽  
Mammary Glands ◽  
Mammary Tissue ◽  
Lactose Synthesis

The rate of fatty acid synthesis from [6-14C]glucose in mammary tissue remained low until parturition at 22 days of gestation and increased 10-fold at 1 day post partum. Administration of progesterone on days 20 and 21 or removal of pups at parturition abolished this increase. In the latter case, administration of prolactin, corticosterone or oxytocin had no stimulatory effect; tissue from suckled glands in which the ducts had been ligated at parturition also showed no increased rate in 24 h. Foetoplacentectomy on day 18 did not stimulate fatty acid synthesis but subsequent suckling by foster pups did. Whereas lactose synthesis is initiated by withdrawal of progesterone from the circulation, a further stimulus related to removal of milk by suckling is required to initiate fatty acid synthesis.

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