scholarly journals Induction and migration of the anterior visceral endoderm is regulated by the extra-embryonic ectoderm

Development ◽  
2005 ◽  
Vol 132 (11) ◽  
pp. 2513-2520 ◽  
Author(s):  
T. A. Rodriguez
Keyword(s):  
Visceral Endoderm ◽  
And Migration ◽  
Embryonic Ectoderm ◽  
Anterior Visceral Endoderm

scholarly journals Characterisation of the transcriptional dynamics underpinning the function, fate, and migration of the mouse Anterior Visceral Endoderm

2021 ◽  
Author(s):  
Shifaan Thowfeequ ◽  
Jonathan Fiorentino ◽  
Di Hu ◽  
Maria Solovey ◽  
Sharon Ruane ◽  
...  
Keyword(s):  
Primitive Streak ◽  
Pharmacological Intervention ◽  
Visceral Endoderm ◽  
Migratory Behaviour ◽  
Tight Coupling ◽  
Transient Nature ◽  
Transcriptional Dynamics ◽  
And Migration ◽  
And Function ◽  
Anterior Visceral Endoderm

During early post-implantation development of the mouse embryo, the Anterior Visceral Endoderm (AVE) differs from surrounding visceral endoderm (VE) in its migratory behaviour and ability to restrict primitive streak formation to the opposite side of the egg cylinder. In order to characterise the molecular basis for the unique properties of the AVE, we combined single-cell RNA-sequencing of the VE prior to and during AVE migration, with high-resolution imaging, short-term lineage labelling, phosphoproteomics and pharmacological intervention. This revealed the transient nature of the AVE, the emergence of heterogeneities in AVE transcriptional states relative to position of cells, and its prominence in establishing gene expression asymmetries within the spatial constraints of the embryo. We identified a previously unknown requirement of Ephrin- and Semaphorin-signalling for AVE migration. These findings point to a tight coupling of transcriptional state and position in the AVE and reveal molecular heterogeneities underpinning its migratory behaviour and function.

scholarly journals Bone morphogenetic protein 4 signaling regulates development of the anterior visceral endoderm in the mouse embryo

2008 ◽  
Vol 50 (7) ◽  
pp. 615-621 ◽  
Author(s):  
Miguel L. Soares ◽  
Maria-Elena Torres-Padilla ◽  
Magdalena Zernicka-Goetz
Keyword(s):  
Bone Morphogenetic Protein ◽  
Mouse Embryo ◽  
Bone Morphogenetic Protein 4 ◽  
Visceral Endoderm ◽  
Morphogenetic Protein ◽  
Anterior Visceral Endoderm

scholarly journals Anterior Visceral Endoderm SMAD4 Signaling Specifies Anterior Embryonic Patterning and Head Induction in Mice

2010 ◽  
pp. 569-583 ◽  
Author(s):  
Cuiling Li ◽  
Yi-Ping Li ◽  
Xin-Yuan Fu ◽  
Chu-Xia Deng
Keyword(s):  
Visceral Endoderm ◽  
Embryonic Patterning ◽  
Anterior Visceral Endoderm

scholarly journals Regionalised signalling within the extraembryonic ectoderm regulates anterior visceral endoderm positioning in the mouse embryo

2006 ◽  
Vol 123 (4) ◽  
pp. 288-296 ◽  
Author(s):  
Lucy Richardson ◽  
Maria-Elena Torres-Padilla ◽  
Magdalena Zernicka-Goetz
Keyword(s):  
Mouse Embryo ◽  
Visceral Endoderm ◽  
Anterior Visceral Endoderm

In vitro development of inner cell masses isolated immunosurgically from mouse blastocysts

Development ◽  
1978 ◽  
Vol 45 (1) ◽  
pp. 93-105
Author(s):  
Brigid Hogan ◽  
Rita Tilly
Keyword(s):  
Inner Cell Mass ◽  
Cell Mass ◽  
Giant Cells ◽  
Epithelial Layer ◽  
Visceral Endoderm ◽  
Limited Region ◽  
In Vitro Development ◽  
Vitro Development ◽  
Embryonic Ectoderm

This paper describes the in vitro development of inner cell masses isolated immunosurgically from mouse blastocysts which had been collected on 3·5 days p.c. and then incubated for 24 h. The inner cell masses continue to grow in culture and develop through a series of stages with increasing complexity of internal organization. By day 1 all of the cultured ICMs have an outer layer of endoderm, and by day 3 some of them have two distinct kinds of inside cells; a columnar epithelial layer and a thin hemisphere of elongated cells. Later, mesodermal cells appear to delaminate from a limited region of the columnar layer, close to where it forms a junction with the thinner cells. By day 5, about 25% of the cultured ICMs have a striking resemblance to normal 7·5-day p.c. C3H embryos, with embryonic ectoderm, extra-embryonic ectoderm and chorion, embryonic and extra-embryonic mesoderm, and visceral endoderm. When mechanically disrupted and grown as attached clumps of cells in a tissue dish, these embryo-like structures give rise to trophoblast-like giant cells. These results suggest that the inner cell mass of 4·5-day p.c. blastocysts contains cells which can give rise to trophoblast derivates in culture.

Localization and synthesis of alphafoetoprotein in post-implantation mouse embryos

Development ◽  
1978 ◽  
Vol 43 (1) ◽  
pp. 289-313
Author(s):  
M. Dziadek ◽  
E. Adamson
Keyword(s):  
Yolk Sac ◽  
Mouse Embryos ◽  
Visceral Endoderm ◽  
Mouse Embryogenesis ◽  
Visceral Yolk Sac ◽  
Radioactive Labelling ◽  
Post Implantation ◽  
Embryonic Ectoderm ◽  
Embryonic Region

The localization and synthesis of alphafoetoprotein (AFP) during mouse embryogenesis were studied by immunoperoxidase and by immunoprecipitation after radioactive labelling, using an antiserum prepared against AFP. AFP is first detectable in embryos on the 7th day of gestation (7th day embryos). In 7th and 8th day embryos AFP is confined to visceral (proximal) endoderm cells around the embryonic region of the egg cylinder. Visceral extra-embryonic and parietal (distal) endoderm cells do not contain AFP. By the 9th day of gestation AFP is also present in the extra-embryonic ectoderm, mesoderm and embryonic ectoderm cells around the three cavities of the embryo. These tissues do not synthesize AFP when cultured in isolation, but can adsorb AFP when it is added to the medium. On the 12th day of gestation AFP synthesis is confined to the endoderm layer of the visceral yolk sac. It is concluded that the ability to synthesize AFP is a property which is restricted to the visceral endoderm during early post-implantation development. The presence of AFP in other tissues of the embryo appears to be due to adsorption.

scholarly journals Concerted cell divisions in embryonic visceral endoderm guide anterior visceral endoderm migration

2019 ◽  
Vol 450 (2) ◽  
pp. 132-140 ◽  
Author(s):  
Francesco Antonica ◽  
Lorenzo Carlo Orietti ◽  
Richard Lester Mort ◽  
Magdalena Zernicka-Goetz
Keyword(s):  
Visceral Endoderm ◽  
Cell Divisions ◽  
Anterior Visceral Endoderm

scholarly journals Correct Patterning of the Primitive Streak Requires the Anterior Visceral Endoderm

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17620 ◽  
Author(s):  
Daniel W. Stuckey ◽  
Aida Di Gregorio ◽  
Melanie Clements ◽  
Tristan A. Rodriguez
Keyword(s):  
Primitive Streak ◽  
Visceral Endoderm ◽  
Anterior Visceral Endoderm
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