Cloning and characterization of a myoblast cell surface antigen defined by 24.1D5 monoclonal antibody

Development ◽  
1989 ◽  
Vol 105 (4) ◽  
pp. 723-731 ◽  
Author(s):  
H.J. Gower ◽  
S.E. Moore ◽  
G. Dickson ◽  
V.L. Elsom ◽  
R. Nayak ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Monoclonal Antibody ◽  
Cell Surface ◽  
Human Skeletal Muscle ◽  
Muscle Development ◽  
Early Stage ◽  
Primary Cultures ◽  
Skeletal Muscle Development ◽  
Mouse Muscle ◽  
Human Skeletal Muscle Cells

Monoclonal antibody 24.1D5 reacts specifically with an epitope expressed on the cell surface of mononucleate myoblasts in primary cultures of human skeletal muscle cells, but not with either multinucleate myotubes or fibroblasts. Polypeptides of 60 and 100 X 10(3) Mr were identified by immunoblotting with the McAb. Human muscle cDNAs encoding the 24.1D5 epitope were used to study further the structure and expression of 24.1D5 during skeletal muscle development. Two mRNA species of 3.0 and 2.5 kb were identified in primary cultures of human skeletal muscle and in mouse muscle cell lines. The levels of both transcripts decreased during myotube formation in vitro and were similarly decreased during myogenesis in the mouse embryo. 24.1D5 mRNAs were expressed by multipotential cells and myoblast derivatives of the mouse embryonic cell line C3H10T1/2, suggesting that 24.1D5 is expressed at an early stage during skeletal muscle development.

scholarly journals Study on the transcriptional regulatory mechanism of the MyoD1 gene in Guanling bovine

RSC Advances ◽  
2018 ◽  
Vol 8 (22) ◽  
pp. 12409-12419
Author(s):  
Di Zhou ◽  
Houqiang Xu ◽  
Wei Chen ◽  
Yuanyuan Wang ◽  
Ming Zhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Regulatory Mechanism ◽  
Early Stage ◽  
Myoblast Differentiation ◽  
Differentiation Process ◽  
Skeletal Muscle Development ◽  
Transcriptional Regulatory Mechanism ◽  
Transcriptional Regulatory

The MyoD1 gene plays a key role in regulating the myoblast differentiation process in the early stage of skeletal muscle development.

Glucose uptake and metabolism by cultured human skeletal muscle cells: rate-limiting steps

2001 ◽  
Vol 281 (1) ◽  
pp. E72-E80 ◽  
Author(s):  
Laureta M. Perriott ◽  
Tetsuro Kono ◽  
Richard R. Whitesell ◽  
Susan M. Knobel ◽  
David W. Piston ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Glucose Metabolism ◽  
Glucose Transport ◽  
Human Skeletal Muscle ◽  
Primary Cultures ◽  
Insulin Response ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Insulin Effect ◽  
Human Skeletal Muscle Cells

To use primary cultures of human skeletal muscle cells to establish defects in glucose metabolism that underlie clinical insulin resistance, it is necessary to define the rate-determining steps in glucose metabolism and to improve the insulin response attained in previous studies. We modified experimental conditions to achieve an insulin effect on 3- O-methylglucose transport that was more than twofold over basal. Glucose phosphorylation by hexokinase limits glucose metabolism in these cells, because the apparent Michaelis-Menten constant of coupled glucose transport and phosphorylation is intermediate between that of transport and that of the hexokinase and because rates of 2-deoxyglucose uptake and phosphorylation are less than those of glucose. The latter reflects a preference of hexokinase for glucose over 2-deoxyglucose. Cellular NAD(P)H autofluorescence, measured using two-photon excitation microscopy, is both sensitive to insulin and indicative of additional distal control steps in glucose metabolism. Whereas the predominant effect of insulin in human skeletal muscle cells is to enhance glucose transport, phosphorylation, and steps beyond, it also determines the overall rate of glucose metabolism.

Impact of SOCS3 overexpression on human skeletal muscle development in vitro

Cytokine ◽  
2011 ◽  
Vol 55 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Marissa K. Caldow ◽  
Gregory R. Steinberg ◽  
David Cameron-Smith
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Development ◽  
Skeletal Muscle Development ◽  
Development In Vitro

Developmental regulation of cell-surface glycoproteins in clonal cultures of human skeletal muscle satellite cells

1989 ◽  
Vol 67 (2-3) ◽  
pp. 128-136 ◽  
Author(s):  
Shashikant Champaneria ◽  
Paul C. Holland ◽  
George Karpati ◽  
Claude Guérin
Keyword(s):  
Skeletal Muscle ◽  
Cell Surface ◽  
Satellite Cells ◽  
Human Skeletal Muscle ◽  
Developmental Regulation ◽  
Lectin Binding ◽  
Primary Cultures ◽  
Surface Proteins ◽  
Cell Surface Glycoproteins ◽  
Surface Glycoproteins

Pure populations of myogenic cells were obtained by cloning satellite cells from human skeletal muscle biopsies. Cell-surface glycoproteins at various stages of myogenesis were analysed by one- and two-dimensional gel electrophoresis. A total of 14 distinct proteins were detectable at the cell surface, on the basis of their susceptibility to desialation by exogenous neuraminidase or their iodination by exogenous lactoperoxidase. Reproducible changes in lectin binding or iodination of eight of these proteins occurred during myogenesis. Only two of the developmentally regulated proteins were components of the detergent-insoluble extracellular matrix fraction. Developmental regulation of these two proteins was unaffected by growth of cultures in 5-bromo-2′-deoxyuridine to inhibit myogenesis. In contrast, developmental regulation of the other cell-surface proteins was inhibited by growth in 5-bromo-2′-deoxyuridine, suggesting that changes in these proteins are tightly coupled to satellite cell differentiation. These studies represent the first systematic analysis of the surface proteins of pure, clonally derived, primary cultures of normal myogenic cells.Key words: satellite cells, myogenesis, myoblast, glycoproteins, cell surface.

TGFß regulates metabolism of human skeletal muscle cells by miRNAs

2018 ◽  
Author(s):  
S Höckele ◽  
P Huypens ◽  
C Hoffmann ◽  
T Jeske ◽  
M Hastreiter ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Human Skeletal Muscle Cells

Effect of insulin on GLUT4 cell surface content and turnover rate in human skeletal muscle as measured by the exofacial bis-mannose photolabeling technique

Diabetes ◽  
1997 ◽  
Vol 46 (12) ◽  
pp. 1965-1969 ◽  
Author(s):  
S. Lund ◽  
G. D. Holman ◽  
J. R. Zierath ◽  
J. Rincon ◽  
L. A. Nolte ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Surface ◽  
Human Skeletal Muscle ◽  
Turnover Rate ◽  
Surface Content
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