Developmental profile of glucose phosphate isomerase allozymes in parthenogenetic and tetraploid mouse embryos

Glucose phosphate isomerase (GPI) allozymes were compared in eggs and embryos of the mouse strains C57BL/6-JHan (GPI-1BB) and 129/Sv (GPI-1AA) under different experimental conditions. The quantitative differences in eggs of the two strains disappeared by the blastocyst stage at day 4 to 5, both in fertilized and diploid parthenogenetic embryos. The degree of degradation of oocyte-coded enzyme molecules and the activation of the embryonic genome for GPI appeared to be equivalent in parthenogenetic embryos from heterozygous females when only one or other maternal allele type remained in the egg after meiosis. Also in tetraploid embryos, generated by electrofusion of homozygous fertilized eggs from the two strains, both genomes seemed to be activated at the same time at day 4; here, however, the GPI-1BB allozyme remained predominant up to day 6.

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Genetically identical parthenogenetic mouse embryos produced by inhibition of the first meiotic cleavage with cytochalasin D

Development ◽

10.1242/dev.111.3.763 ◽

1991 ◽

Vol 111 (3) ◽

pp. 763-769 ◽

Cited By ~ 1

Author(s):

J. Kubiak ◽

A. Paldi ◽

M. Weber ◽

B. Maro

Keyword(s):

Meiotic Division ◽

Polar Body ◽

Blastocyst Stage ◽

Cytochalasin D ◽

Meiotic Spindle ◽

Glucose Phosphate ◽

First Polar Body ◽

Histone Kinase ◽

Parthenogenetic Mouse Embryos ◽

Parthenogenetic Embryos

The microfilament inhibitor cytochalasin D inhibits extrusion of the first polar body when present during the first meiotic division of mouse oocytes; however, it does not interfere with anaphase movement of chromosomes, and thus induces the formation of tetraploid oocytes. After the separation of chromosomes in anaphase, two spindles start to assemble. However, they merge rapidly and a single meiotic spindle forms. During the transition between metaphase I and metaphase II, in the presence of cytochalasin D, a drop in histone kinase activity takes place demonstrating a transitional decrease in the activity of the maturation promoting factor. These oocytes can be activated parthenogenetically a few hours after washing out the inhibitor. After completion of the second meiotic division and extrusion of a polar body, they contain a diploid number of chromosomes. They are genetically identical to each other and to their mother. Such eggs develop to the blastocyst stage and can implant in the uteri of foster mothers. Most of these fetuses die before the 9th day of gestation, as do diploid control fetuses treated with cytochalasin D during the second meiotic division. The heterozygous state of the experimental embryos obtained after activation of eggs recovered from heterozygous females and treated with cytochalasin D during the first meiotic division was confirmed using a glucose-phosphate isomerase assay. This technique allows the production of genetic clones of parthenogenetic embryos by simple means.

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Cell-Free Translation of Messenger RNAs from Adult and Fetal Human Muscle. Characterization or Neosynthesized Glycogen Phosphorylase, Phosphofructokinase and Glucose Phosphate Isomerase

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1981.tb05293.x ◽

1981 ◽

Vol 116 (1) ◽

pp. 7-12 ◽

Cited By ~ 46

Author(s):

Axel KAHN ◽

Dominique COTTREAU ◽

Dominique DAEGELEN ◽

Jean-Claude DREYFUS

Keyword(s):

Glycogen Phosphorylase ◽

Human Muscle ◽

Messenger Rnas ◽

Glucose Phosphate Isomerase ◽

Glucose Phosphate ◽

Free Translation

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Characterization of the 5′ end of the gene for human glucose phosphate isomerase (GPI)

Genomics ◽

10.1016/0888-7543(90)90212-d ◽

1990 ◽

Vol 7 (4) ◽

pp. 638-643 ◽

Cited By ~ 12

Author(s):

James I.H. Walker ◽

Pelin Faik ◽

Michael J. Morgan

Keyword(s):

Glucose Phosphate Isomerase ◽

Glucose Phosphate

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Comparison of human and murine isolates of Schistosoma mansoni from Richard-Toll, Senegal, by isoelectric focusing

Journal of Helminthology ◽

10.1017/s0022149x00015868 ◽

1997 ◽

Vol 71 (2) ◽

pp. 175-181 ◽

Cited By ~ 7

Author(s):

M. Sène ◽

P. Brémond ◽

J.P. Hervé ◽

V.R. Southgate ◽

B. Sellin ◽

...

Keyword(s):

Genetic Variation ◽

Acid Phosphatase ◽

Lactate Dehydrogenase ◽

Schistosoma Mansoni ◽

Isoelectric Focusing ◽

Glucose Phosphate Isomerase ◽

Glucose Phosphate ◽

Enzyme Systems ◽

Glucose 6 Phosphate Dehydrogenase ◽

Arvicanthis Niloticus

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.

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Synthesis and phosphorylation of uvomorulin during mouse early development

Development ◽

10.1242/dev.115.1.313 ◽

1992 ◽

Vol 115 (1) ◽

pp. 313-318 ◽

Cited By ~ 2

Author(s):

M. Sefton ◽

M.H. Johnson ◽

L. Clayton

Keyword(s):

Cell Adhesion ◽

Early Development ◽

Adhesion Molecule ◽

Cell Adhesion Molecule ◽

Blastocyst Stage ◽

Embryonic Stage ◽

Cell Stage ◽

Mouse Oocytes ◽

Maternal Mrna ◽

Embryonic Genome

The cell adhesion molecule, uvomorulin, is synthesised in both the 135 × 10(3) M(r) precursor and 120 × 10(3) M(r) mature forms on maternal mRNA templates in unfertilized and newly fertilized mouse oocytes. Synthesis on maternal message ceases during the 2-cell stage to resume later on mRNA encoded presumptively by the embryonic genome. Uvomorulin is detectable by immunoblotting at all stages upto the blastocyst stage, but shows variations in its total amount and processing with embryonic stage. Whilst only trace levels of phosphorylated uvomorulin are detectable in early and late 4-cell embryos, uvomorulin in 8-cell embryos is phosphorylated.

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