Comparison of human and murine isolates of Schistosoma mansoni from Richard-Toll, Senegal, by isoelectric focusing

1997 ◽  
Vol 71 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Sène ◽  
P. Brémond ◽  
J.P. Hervé ◽  
V.R. Southgate ◽  
B. Sellin ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Schistosoma Mansoni ◽  
Isoelectric Focusing ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Arvicanthis Niloticus

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.

Isoelectric focusing of general protein and specific enzymes from pathotypes of Globodera rostochiensis and G. pallida

Parasitology ◽  
1984 ◽  
Vol 88 (1) ◽  
pp. 131-139 ◽  
Author(s):  
P. C. Fox ◽  
H. J. Atkinson
Keyword(s):  
Isoelectric Focusing ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Species Variation ◽  
Glucose Phosphate Isomerase ◽  
Protein Patterns ◽  
Major Band ◽  
Glucose Phosphate ◽  
General Protein ◽  
And Cluster Analysis

SUMMARYVariations in the general protein and 40 enzymes of the 8 European pathotypes of the potato cyst nematode Globodera rostochiensis and G. pallida have been examined by isoelectric focusing (IEF) and cluster analysis. Differences in the protein patterns were observed and the presence of a major band at pH 8·0 allowed the two species to be discriminated. Extra isozymed of peptidase (EC 3.4. 11.*) and esterase (EC 3. 1. 1. 1) were also found in G. rostochiensis. Intra-species variation was seen within G. rostochiensis for superoxide dismutase (EC 1. 15. 1. 1) and within G. pallida for phosphoglucomutase (EC 2. 7. 5. 1) and glucose phosphate isomerase (EC 5. 3. 1. 9). The results suggest that the two species are very similar and the prospect for recognizing their pathotypes by IEF is discussed.

scholarly journals Detection of Glucose-Phosphate Isomerase Deficiency by a Screening Procedure

Blood ◽  
1972 ◽  
Vol 39 (5) ◽  
pp. 685-687 ◽  
Author(s):  
Karl-Georg Blume ◽  
Ernest Beutler
Keyword(s):  
Rapid Detection ◽  
Screening Procedure ◽  
Red Cell ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Dehydrogenase Reaction ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Cell Glucose

Abstract A method for rapid detection of red cell glucose-phosphate isomerase deficiency is described. The procedure is based on the appearance of fluorescence, caused by TPNH, that is generated in the linked glucose-phosphate isomerase/glucose-6-phosphate dehydrogenase reaction.

INHERITANCE OF ALLOZYME VARIANTS IN COASTAL DOUGLAS-FIR (PSEUDOTSUGA MENZIESII VAR. MENZIESII)

1982 ◽  
Vol 24 (3) ◽  
pp. 325-335 ◽  
Author(s):  
Y. A. El-Kassaby ◽  
F. C. Yeh ◽  
O. Sziklai
Keyword(s):  
Superoxide Dismutase ◽  
Acid Phosphatase ◽  
Pseudotsuga Menziesii ◽  
Malic Enzyme ◽  
Quaternary Structure ◽  
Allelic Variation ◽  
Enzyme System ◽  
Douglas Fir ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase

The inheritance of 27 loci from 18 enzyme systems was investigated using both megagametophyte and embryo tissues of open pollinated seed collected from a natural stand of coastal Douglas-fir [Pseudotsuga menziesii var. menziesii (Mirb.) Franco]. Four enzyme systems - glutamate dehydrogenase (GDH), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mannose-6-phosphate isomerase (MPI), and peptidase (PEP) - appeared to be monomorphic and the remaining 14 systems - acid phosphatase (APH), aconitase (ACO), aspartate aminotransferase (AAT), diaphorase (DIA), esterase (EST), glucose-6-phosphate dehydrogenase (G6P), hexoseaminidase (HA), isocitrate dehydrogenase (IDH), malate dehydrogenase (MDH), malic enzyme (ME), phosphoglucose isomerase (PGI), phosphoglucomutase (PGM), 6-phosphogluconic dehydrogenase (6-PGD), and superoxide dismutase (SOD)-showed polymorphism. Each enzyme system was discussed with respect to its multilocus organization, subunit (quaternary) structure, and allelic variation.

scholarly journals Enzyme histochemistry of developing rat oral mucosa.

1981 ◽  
Vol 29 (1) ◽  
pp. 57-64 ◽  
Author(s):  
S Sjögren ◽  
L Hammarström ◽  
A Larsson
Keyword(s):  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Succinate Dehydrogenase ◽  
Oral Mucosa ◽  
Adenosine Triphosphatase ◽  
Enzyme Histochemistry ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Developing Rat

The oral mucosa of developing and mature rats was analyzed histochemically for regional enzyme differences. The following enzymes were studied: nonspecific alkaline phosphatase (alkpase), acid phosphatase (acidpase), 5'-nucleotidase (AMPase), adenosine triphosphatase (ATPase), succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), and glucose-6-phosphate dehydrogenase (G-6-pDH). All enzymes were active in the oral mucosa, but regional as well as tissue variations were observed. Epithelium in all regions showed acidpase staining. Oxidoreductases were found in all regions with variations within the epithelium. The epithelium of specific regions stained for alkpase and AMPase, while adjacent epithelium did not. We suggest that the alkpase and AMPase activities are associated with specific functions of the epithelium in these regions.

ELECTROPHORETIC STUDY OF ENZYMES FROM A GLOSSINA FUSCIPES FUSCIPES NEWSTEAD POPULATION FROM WESTERN KENYA

1991 ◽  
Vol 123 (2) ◽  
pp. 295-298 ◽  
Author(s):  
G.F. Rajendram
Keyword(s):  
Lake Victoria ◽  
Xanthine Dehydrogenase ◽  
Autosomal Locus ◽  
Glucose Phosphate Isomerase ◽  
Electrophoretic Study ◽  
Western Kenya ◽  
Glossina Fuscipes Fuscipes ◽  
Glucose Phosphate ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Rusinga Island

AbstractEnzymes were investigated, by electrophoresis, in a population of Glossina fuscipes fuscipes Newstead collected from Rusinga Island in Lake Victoria, Western Kenya.The following enzymes were tested: glucose phosphate isomerase, glucose-6-phosphate dehydrogenase (G6PDH), hexokinase. isocitrate dehydrogenase (IDH), malate-dehydrogenase (MDH), phosphoglucomutase, and xanthine dehydrogenase (XDH).Single monomorphic bands were stained by the following enzymes apparently under the control of single loci: G6PDH, MDH, and XDH. The enzyme IDH showed two bands with very close mobilities and no variation among individuals in the population. Hence IDH was considered as representing a single locus. Glucose phosphate isomerase manifested three alleles and apparently six genotypes. Phosphoglucomutase manifested a double-banded pattern representing an autosomal locus.

scholarly journals The effect of dietary and hormonal conditions on the activities of glycolytic enzymes in rat epididymal adipose tissue

1969 ◽  
Vol 115 (3) ◽  
pp. 405-417 ◽  
Author(s):  
E. D. Saggerson ◽  
A. L. Greenbaum
Keyword(s):  
Adipose Tissue ◽  
Lactate Dehydrogenase ◽  
Pyruvate Kinase ◽  
High Fat Diet ◽  
Alloxan Diabetes ◽  
Phosphoglycerate Kinase ◽  
Glycolytic Enzymes ◽  
Glucose Phosphate Isomerase ◽  
High Fat ◽  
Glucose Phosphate

1. Measurements were made of the activities of nine glycolytic enzymes in epididymal adipose tissues obtained from rats that had undergone one of the following treatments: starvation; starvation followed by re-feeding with bread or high-fat diet; feeding with fat without preliminary starvation; alloxan-diabetes; alloxan-diabetes followed by insulin therapy. 2. In general, the activities of the glycolytic enzymes of adipose tissue, unlike those of liver, were not greatly affected by the above treatments. 3. The ‘key’ glycolytic enzymes, phosphofructokinase and pyruvate kinase, were generally no more adaptive in response to physiological factors than other glycolytic enzymes such as glucose phosphate isomerase, fructose diphosphate aldolase, triose phosphate isomerase, glycerol 3-phosphate dehydrogenase, phosphoglycerate kinase and lactate dehydrogenase. 4. Adiposetissue pyruvate kinase did not respond to feeding with fat in a manner similar to the liver enzyme. 5. Glyceraldehyde phosphate dehydrogenase had a behaviour pattern unlike the other eight glycolytic enzymes studied in that its activity was depressed by feeding with fat and was not restored to normal by re-feeding with a high-fat diet after starvation. These results are discussed in relation to the requirements of adipose tissue for glycerol phosphate in the esterification of fatty acids. 6. A statistical analysis of the results permitted the writing of linear equations describing the relationships between the activities of eight of the enzymes studied. 7. Evidence is presented for the existence of two constant-proportion groups amongst the enzymes studied, namely (i) glucose phosphate isomerase, phosphoglycerate kinase and lactate dehydrogenase, and (ii) triose phosphate isomerase, fructose diphosphate aldolase and pyruvate kinase. 8. Mechanisms for maintaining the observed relationships between the activities of the enzymes in the tissue are discussed.

scholarly journals Influence of long-term using of soft contact lenses on indicators of oxidative-reducing potential of glutathione, peroxidation of lipids, and the state of cell membranes and cellular structures of the epithelium of the cornea

2018 ◽  
Vol 22 (1) ◽  
pp. 146-151
Author(s):  
T.A. Veliksar ◽  
M.F. Leus ◽  
T.B. Haydamaka ◽  
I.M. Mykheitseva ◽  
G.I. Drozhzhina ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Cell Membranes ◽  
Contact Lenses ◽  
Control Group ◽  
Soft Contact ◽  
Soft Contact Lenses ◽  
Glucose 6 Phosphate Dehydrogenase

Contact lenses are increasingly used worldwide for correcting refraction disorders. However, contact lenses can destroy a biochemical composition of the tear. Purpose — to determine the effect of long-term use of silicone-hydrogel contact lenses on the parameters of the oxidation-reduction potential of glutathione, lipid peroxidation and the stability of cell membranes and subcellular structures of the cornea of the epithelium by identifying marker enzymes in a tear. We determined the activity of lactate dehydrogenase, glucose-6-phosphate dehydrogenase, acid phosphatase, succinatе dehydrogenase, glutathione (recovered and oxidized forms) and products of lipid peroxidation (malonic dialdehyde, diene conjugates) in tears of patients divided into two groups. Study group comprised 72 people — mild and moderate myopia patients continuously wearing soft contact lenses. Control group consisted of 33 people — mild and moderate myopia patients, spectacles wearers. We revealed a significant increase in the activity of lactate dehydrogenase and glucose-6-phosphate dehydrogenase by >33% and >26%, respectively, as well as a significant increase in the activity of acid phosphatase by >21% and of succinatе dehydrogenase by >18% in the tudy group patients comparing to controls. The study of the level of products of lipid peroxidation in the tear fluid of the main group showed a significant accumulation of them, as well as a violation of the glutathione balance in comparison with the control group. The dependence of biochemical changes in the composition of the tear on the duration of contact lens wear has been revealed. Therefore, with prolonged wearing of MKL, prooxidant-antioxidant equilibrium in the tissues of the eye surface, in particular in the cornea, is violated, activation of free radical processes and reduction of antioxidant reserves occur, which leads to destruction of cellular and subcellular membranes. We assume that the medical correction of these pathological changes will help prevent the development of severe complications of contact correction.

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