Haematology

Anaemia?, Clinical features of anaemia?, Iron-deficiency anaemia?, Anaemia of inflammation?, Macrocytic anaemias?, Haemolytic anaemias?, Red cell membranopathies?, Glucose--phosphate dehydrogenase deficiency?, Sickle cell anaemia?, Thalassaemia?, Blood transfusion?, Effects of HIV/AIDS?, Acute leukaemias?, Lymphoproliferative disorders?, Myeloproliferative disorders?, Splenomegaly?, Disorders of haemostasis?, Acquired coagulation disorders?, Laboratory issues?

The effect of drone transport on the stability of biochemical, coagulation and hematological parameters in healthy individuals

Objectives Transport of blood tubes is mainly by car or pneumatic transport. The transportation of blood tubes by drones is a novel approach for rapid transportation of blood tubes over long distances. However, limited data on the stability of biochemical, coagulation and hematological parameters is available after transport of blood tubes by drone. Methods To investigate the effect of drone transport on the stability of blood parameters, four test flights were performed. Blood was drawn from 20 healthy individuals and 39 of the most frequently measured blood parameters were compared between 4 groups; immediate measurement (control), late measurement, transport by car and transport by drone. Total Allowable Error (TAE) of the EFLM Biological Variation Database was used to determine the clinical relevance of significant differences. Results The majority of blood parameters were not affected by drone transport. Eight of the measured parameters showed significant differences between all the groups; glucose, phosphate, potassium, chloride, hemoglobin, platelet count, APTT and Lactate dehydrogenase (LD). A clinically relevant increase for LD after transport and a decrease for glucose values in time and after transport compared with the control group was shown. Conclusions Transportation of blood tubes from healthy individuals by drones has a limited clinically relevant effect. From the 39 investigated blood parameters only LD and glucose showed a clinically relevant effect.

Fanconi Syndrome Leading to Hypophosphatemic Osteomalacia Related to Tenofovir Use

Tenofovir disoproxil fumarate (TDF) is used worldwide to treat and prevent Human Immunodeficiency Virus (HIV) infection. Fanconi syndrome is a complication of TDF use and is characterized by inadequate reabsorption of glucose, phosphate and protein in the proximal tubule of the kidney which may eventually lead to osteomalacia manifested by symptoms of pain, muscular weakness and difficulty ambulating. We present a patient with severe osteomalacia due to progressive and unrecognized Fanconi’s syndrome, who responded rapidly to TDF withdrawal, oral phosphate repletion and calcitriol. With the widespread use of TDF-containing antiviral regimens, it is critically important that physicians adhere to screening recommendations to detect early Fanconi syndrome, and recognize symptoms of osteomalacia as a serious complication.

The transfer hydrogenation of high concentration levulinic acid to γ-valerolactone catalyzed by glucose phosphate carbamide zirconium

Newly constructed glucose phosphate carbamide zirconium (GluPC-Zr) is an inexpensive, excellent, and recyclable CTH catalyst for the synthesis of γ-valerolactone (γ-GVL) using high concentrations of levulinic acid (LA ).

Stochastic Analysis Demonstrates the Dual Role of Hfq in Chaperoning E. coli Sugar Shock Response

Small RNAs (sRNAs) play a crucial role in the regulation of bacterial gene expression by silencing the translation of target mRNAs. SgrS is an sRNA that relieves glucose-phosphate stress, or “sugar shock” in E. coli. The power of single cell measurements is their ability to obtain population level statistics that illustrate cell-to-cell variation. Here, we utilize single molecule super-resolution microscopy in single E. coli cells coupled with stochastic modeling to analyze glucose-phosphate stress regulation by SgrS. We present a kinetic model that captures the combined effects of transcriptional regulation, gene replication and chaperone mediated RNA silencing in the SgrS regulatory network. This more complete kinetic description, simulated stochastically, recapitulates experimentally observed cellular heterogeneity and characterizes the binding of SgrS to the chaperone protein Hfq as a slow process that not only stabilizes SgrS but also may be critical in restructuring the sRNA to facilitate association with its target ptsG mRNA.

Influence of the butylparaben administration on the oxidative stress metabolism of liver, kidney and spleen

ObjectivesButylparaben is widely used synthetic polymer as preservative in the food, pharmaceutical and cosmetic industries. Although butylparaben is metabolized in the detoxification organs including liver and kidney, some parts of it can retain and accumulate in the body. Parabens can impair developmental and reproductive health, though there is not any published data related with the influence of the butylparaben on the oxidative stress metabolism in the detoxification organs. Therefore, we aimed to evaluate antioxidant enzyme activities in the liver, kidney and spleen of butylparaben-treated rat.MethodsPrepubertal Wistar albino male rats were administered with 0, 100, 200, 400 mg/kg/day butylparaben for 28 days. After treatment, enzyme activities were evaluated as the biomarkers of the oxidative stress.ResultsEnzyme activities including glucose-phosphate dehydrogenase (G6PD), 6-phosphoglucanate dehydrogenase (6-PGD), glutathione reductase (GR), glutathione s-transferase (GST) and glutathione peroxidase (GPx) were impaired upon butylparaben treatment in the liver, kidney and spleen tissues.ConclusionsExposure to endocrine disruptors may affect enzyme activities of the detoxification organs and change the pentose phosphate glutathione (GSH) metabolisms. According to our data oxidative stress metabolism is impaired in the spleen, kidney and liver tissue upon butylparaben treatment that has been indicated first time in the literature.

Stochastic Analysis Demonstrates the Dual Role of Hfq in Chaperoning E. coli Sugar Shock Response

Small RNAs (sRNAs) play a crucial role in the regulation of bacterial gene expression by silencing the translation of target mRNAs. SgrS is an sRNA that relieves glucose-phosphate stress, or “sugar shock” in E. coli. The power of single cell measurements is their ability to obtain population level statistics that illustrate cell-to-cell variation. Here, we utilize single molecule super-resolution microscopy in single E. coli cells coupled with stochastic modeling to analyze glucose-phosphate stress regulation by SgrS. We present a kinetic model that captures the combined effects of transcriptional regulation, gene replication and chaperone mediated RNA silencing in the SgrS regulatory network. This more complete kinetic description, simulated stochastically, recapitulates experimentally observed cellular heterogeneity and characterizes the binding of SgrS to the chaperone protein Hfq as a slow process that not only stabilizes SgrS but also may be critical in restructuring the sRNA to facilitate association with its target ptsG mRNA.