Development of explanted rat embryos in circulating medium

Development ◽  
1967 ◽  
Vol 17 (3) ◽  
pp. 513-525
Author(s):  
D. A. T. New
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Rat Embryos ◽  
Embryo Extract ◽  
Tissue Culture Method

The inaccessibility of rat embryos in vivo has prompted several previous attempts to grow them in culture. It has been shown that embryos explanted at head fold or early somite stages with their membranes will develop on the surface of plasma-embryo extract clots (New & Stein, 1964; New, 1966 a) or in homologous liquid plasma or serum (Nicholas & Rudnick, 1934, 1938; Jolly & Lieure, 1938; New, 1966a, b). The explants can conveniently be cultured in watch glasses, as in the standard tissue culture method of Fell & Robison (1929), and development is improved if they are incubated in 60–90% O2 and 3–5% CO2. Under these conditions the older embryos grow well for 30–40 h developing to 25- to 30-somite stages. However, no embryos have been observed to develop much further than this, and those explanted at stages more advanced than 30 somites have always died fairly quickly.

scholarly journals In Vitro Effect of Erythropoietin on the Spleen of The Polycythemic Mouse

Blood ◽  
1967 ◽  
Vol 29 (6) ◽  
pp. 852-858
Author(s):  
YASUSADA MIURA ◽  
FUMIMARO TAKAKU ◽  
KIKU NAKAO
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Stem Cell Differentiation ◽  
Mouse Spleen ◽  
In Vitro Method ◽  
Heme Synthesis ◽  
Tissue Culture Method ◽  
Vitro Effect

Abstract 1. An in vitro method to observe radiosensitivity of stem cells was developed in the present study. In vivo and in vitro effect of 60Co irradiation on the erythropoietin-induced stem cell differentiation into erythroblasts was observed, using a tissue culture method of polycythemic mouse spleen. Response to erythropoietin was demonstrated by an appearance of heme synthesis and erythroblasts in spleen fragments. 2. A significant correlation between the rate of appearance of erythroblasts and heme synthesis of the spleen fragments was observed. 3. After irradiation, marked impairment of both heme synthesis and production of erythroblasts was observed, yielding D37 values in the vicinity of 70 r in vivo and 120 r in vitro irradiation, respectively. 4. Marked recovery of erythropoietin-induced heme synthesis in the polycythemic mouse spleen was observed 9 days after 300 r irradiation, with an "overshooting" phenomenon on the 12th day.

scholarly journals Assessment of bioscompatibility of isocyanurate-containing polyurethanes with ifosphamide by in vivo and in vitro methods.

Morphologia ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 39-47
Author(s):  
N.A. Galatenko ◽  
D.V. Kuliesh ◽  
R.A. Rozhnova ◽  
V.P. Gritsenko ◽  
L.F. Narazhayko
Keyword(s):  
Tissue Culture ◽  
Composite Materials ◽  
Polymeric Composite ◽  
Culture Method ◽  
Experimental Animals ◽  
Polymeric Composite Materials ◽  
In Vitro Methods ◽  
Tissue Culture Method

Background. The creation of polymeric composite materials with pronounced biological activity, which are able to act as implants with local prolonged action of the immobilized substance can be widely used in medical practice. Objective. Study of cellular reactions of surrounding tissues of experimental animals to implantation of polymeric composite materials based on isocyanurate-containing polyurethanes with ifosfamide, study of histotoxicity of the obtained materials by tissue culture method. Methods. Polymeric composite materials based on isocyanate-containing polyurethanes without and with ifosfamide were implanted into the body of white laboratory Wistar rats. Cellular responses of the organism and possible changes in the structure of test specimens after implantation were studied by light microscopy by analysis of histological micropreparations. In order to study the peculiarities of the dynamics of growth and development of fibroblastic elements, the method of tissue culture was used. Results. Conducted biological studies by in vivo and in vitro methods allowed to evaluate the effect of immobilized ifosfamide in the structure of isocyanurate-containing polyurethanes on cellular reactions of surrounding tissues during implantation in experimental animals, as well as the effect of extracts from developed polymer samples on cultured cell growth. Conclusion. It was found that the implantation of isocyanurate-containing polyurethanes with ifosfamide led to the development of intense cellular reactions in the area of implant placement, primarily the reaction of round cell elements. The presence of ifosfamide in the structure of the polymeric implantation material probably affected the proliferation of cellular elements, inhibited regenerative processes in the early stages of the study and delayed the formation of a mature connective tissue capsule around the implanted samples. The tissue culture method showed that when making an extract of isocyanurate-containing polyurethanes with ifosfamide in the culture medium, there was a large variability of cell forms, which led to the appearance of macrophage-like elements.

scholarly journals Rapid Tissue Culture Method for Detection of Mycoplasma hyorhinis

Microbiology ◽  
1982 ◽  
Vol 128 (11) ◽  
pp. 2817-2820
Author(s):  
J. A. Levy ◽  
P. E. Sumner ◽  
L. E. Hooser
Keyword(s):  
Tissue Culture ◽  
Culture Method ◽  
Mycoplasma Hyorhinis ◽  
Tissue Culture Method

scholarly journals Estimation of Biocompatibility of High Strength β-Tricalcium Phosphate Ceramics by a Tissue Culture Method

1990 ◽  
Vol 98 (1136) ◽  
pp. 404-407 ◽  
Author(s):  
Motohiro TORIYAMA ◽  
Sukezo KAWAMURA ◽  
Yukari KAWAMOTO ◽  
Takahiro SUZUKI ◽  
Yoshiyuki YOKOGAWA ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Tricalcium Phosphate ◽  
High Strength ◽  
Culture Method ◽  
Tissue Culture Method

scholarly journals Vast propagation of submerged tool species Hydrilla verticillata with tissue culture method

2008 ◽  
Vol 20 (2) ◽  
pp. 215-220
Author(s):  
JIANG Jinhui ◽  
◽  
ZHOU Changfang ◽  
AN Shuqing ◽  
GUAN Baohua ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Hydrilla Verticillata ◽  
Culture Method ◽  
Tissue Culture Method

Toxin action b. Novyi (b. Oedematiens Weinberg-Sequin) on tissue growth in vitro and on cell metabolism. To the study of anaerobes and their toxin using the explantation method

1935 ◽  
Vol 31 (2) ◽  
pp. 271-272
Author(s):  
G. Barg
Keyword(s):  
Tissue Culture ◽  
Cell Metabolism ◽  
Anaerobic Bacteria ◽  
Culture Method ◽  
Tissue Growth ◽  
Tissue Culture Method ◽  
Series Of Experiments

The author has shown in a series of experiments that the tissue culture method is quite applicable for the study of anaerobic bacteria and their toxins, especially those of them that are significantly weakened by filtration.

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