scholarly journals Correction: ETHYLENE INSENSITIVE 3 suppresses plant de novo root regeneration from leaf explants and mediates age-regulated regeneration decline

Development ◽  
2021 ◽  
Vol 148 (9) ◽  
Author(s):  
Hong Li ◽  
Lulu Yao ◽  
Lili Sun ◽  
Ziqiang Zhu
Keyword(s):  
De Novo ◽  
Leaf Explants ◽  
Root Regeneration

Control of de novo root regeneration efficiency by developmental status of Arabidopsis leaf explants

2019 ◽  
Vol 46 (3) ◽  
pp. 133-140 ◽  
Author(s):  
Jing Pan ◽  
Fei Zhao ◽  
Guifang Zhang ◽  
Yu Pan ◽  
Lijun Sun ◽  
...  
Keyword(s):  
De Novo ◽  
Leaf Explants ◽  
Developmental Status ◽  
Root Regeneration ◽  
Regeneration Efficiency

scholarly journals ETHYLENE INSENSITIVE 3 suppresses plant de novo root regeneration from leaf explants and mediates age-regulated regeneration decline

Development ◽  
2020 ◽  
Vol 147 (9) ◽  
pp. dev179457 ◽  
Author(s):  
Hong Li ◽  
Lulu Yao ◽  
Lili Sun ◽  
Ziqiang Zhu
Keyword(s):  
De Novo ◽  
Leaf Explants ◽  
Root Regeneration

The Molecular Basis of Age-Modulated Plant De Novo Root Regeneration Decline in Arabidopsis thaliana

2020 ◽  
Author(s):  
Lili Sun ◽  
Ziqiang Zhu
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Fate ◽  
Adventitious Roots ◽  
De Novo ◽  
Leaf Explants ◽  
Auxin Biosynthesis ◽  
Regeneration Capacity ◽  
Exogenous Hormone ◽  
Root Regeneration ◽  
Wox Genes

Abstract Plants possess a regeneration capacity that enables them to survive after wounding. For example, detached Arabidopsis thaliana leaves are able to form adventitious roots from their cutting sites even in the absence of exogenous hormone supplements, as process termed de novo root regeneration (DNRR). Wounding rapidly induces auxin biosynthesis at the cutting sites and then elicits a signaling cascade to promote cell fate transitions and finally generate the adventitious roots. However, rooting rates in older plants are much lower than in younger leaf explants. In this review, we highlight the recent breakthroughs in the understanding of DNRR decay in older plants from at least two independent signaling routes: (i) via the accumulation of EIN3 protein in older plants, which directly suppresses expression of WUSCHEL RELATED HOMEOBOX (WOX) genes to inhibit rooting; (ii) the miR156-SPLs-AP2/ERFs pathway, which modulates root regeneration by reducing auxin biosynthesis.

scholarly journals Molecular Basis for Natural Vegetative Propagation via Regeneration in North American Lake Cress, Rorippa aquatica (Brassicaceae)

2019 ◽  
Vol 61 (2) ◽  
pp. 353-369 ◽  
Author(s):  
Rumi Amano ◽  
Hokuto Nakayama ◽  
Risa Momoi ◽  
Emi Omata ◽  
Shizuka Gunji ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Vegetative Propagation ◽  
Molecular Basis ◽  
Auxin Transport ◽  
Vascular Tissue ◽  
De Novo ◽  
Leaf Explants ◽  
Experimental Models ◽  
Polar Auxin Transport ◽  
Proximal Side

Abstract Some plant species have a striking capacity for regeneration in nature, including regeneration of the entire individual from explants. However, due to the lack of suitable experimental models, the regulatory mechanisms of spontaneous whole plant regeneration are mostly unknown. In this study, we established a novel model system to study these mechanisms using an amphibious plant within Brassicaceae, Rorippa aquatica, which naturally undergoes vegetative propagation via regeneration from leaf fragments. Morphological and anatomical observation showed that both de novo root and shoot organogenesis occurred from the proximal side of the cut edge transversely with leaf vascular tissue. Time-series RNA-seq analysis revealed that auxin and cytokinin responses were activated after leaf amputation and that regeneration-related genes were upregulated mainly on the proximal side of the leaf explants. Accordingly, we found that both auxin and cytokinin accumulated on the proximal side. Application of a polar auxin transport inhibitor retarded root and shoot regeneration, suggesting that the enhancement of auxin responses caused by polar auxin transport enhanced de novo organogenesis at the proximal wound site. Exogenous phytohormone and inhibitor applications further demonstrated that, in R. aquatica, both auxin and gibberellin are required for root regeneration, whereas cytokinin is important for shoot regeneration. Our results provide a molecular basis for vegetative propagation via de novo organogenesis.

scholarly journals YUCCA-Mediated Biosynthesis of the Auxin IAA Is Required during the Somatic Embryogenic Induction Process in Coffea canephora

2020 ◽  
Vol 21 (13) ◽  
pp. 4751
Author(s):  
Miguel A. Uc-Chuc ◽  
Cleyre Pérez-Hernández ◽  
Rosa M. Galaz-Ávalos ◽  
Ligia Brito-Argaez ◽  
Víctor Aguilar-Hernández ◽  
...  
Keyword(s):  
De Novo ◽  
Leaf Explants ◽  
Coffea Canephora ◽  
Naphthaleneacetic Acid ◽  
Induction Process ◽  
Exogenous Addition ◽  
Pre Treatment ◽  
Process Of Se ◽  
Somatic Embryogenic ◽  
Indole 3 Acetic Acid

Despite the existence of considerable research on somatic embryogenesis (SE), the molecular mechanism that regulates the biosynthesis of auxins during the SE induction process remains unknown. Indole-3-acetic acid (IAA) is an auxin that is synthesized in plants through five pathways. The biosynthetic pathway most frequently used in this synthesis is the conversion of tryptophan to indol-3-pyruvic acid (IPA) by tryptophan aminotransferase of Arabidopsis (TAA) followed by the conversion of IPA to IAA by enzymes encoded by YUCCA (YUC) genes of the flavin monooxygenase family; however, it is unclear whether YUC-mediated IAA biosynthesis is involved in SE induction. In this study, we report that the increase of IAA observed during SE pre-treatment (plants in MS medium supplemented with 1-naphthaleneacetic acid (NAA) 0.54 µM and kinetin (Kin) 2.32 µM for 14 days) was due to its de novo biosynthesis. By qRT-PCR, we demonstrated that YUC gene expression was consistent with the free IAA signal found in the explants during the induction of SE. In addition, the use of yucasin to inhibit the activity of YUC enzymes reduced the signal of free IAA in the leaf explants and dramatically decreased the induction of SE. The exogenous addition of IAA restored the SE process in explants treated with yucasin. Our findings suggest that the biosynthesis and localization of IAA play an essential role during the induction process of SE in Coffea canephora.

scholarly journals ABI3 plays a role in de-novo root regeneration from Arabidopsis thaliana callus cells

2020 ◽  
Vol 15 (9) ◽  
pp. 1794147
Author(s):  
Sourabh Sengupta ◽  
Ronita Nag Chaudhuri
Keyword(s):  
Arabidopsis Thaliana ◽  
De Novo ◽  
Root Regeneration

TAA family contributes to auxin production during de novo regeneration of adventitious roots from Arabidopsis leaf explants

2016 ◽  
Vol 61 (22) ◽  
pp. 1728-1731 ◽  
Author(s):  
Beibei Sun ◽  
Lyuqin Chen ◽  
Jingchun Liu ◽  
Xuening Zhang ◽  
Zhongnan Yang ◽  
...  
Keyword(s):  
Adventitious Roots ◽  
De Novo ◽  
Leaf Explants ◽  
Auxin Production
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