Hormone Orchestrates a Hierarchical Transcriptional Cascade That Regulates Al-Induced De Novo Root Regeneration in Tea Nodal Cutting

2021 ◽  
Author(s):  
Long Cheng ◽  
Huan Liu ◽  
Jing Zhao ◽  
Yuan Dong ◽  
Qingshan Xu ◽  
...  
Keyword(s):  
De Novo ◽  
Root Regeneration ◽  
Nodal Cutting

Control of de novo root regeneration efficiency by developmental status of Arabidopsis leaf explants

2019 ◽  
Vol 46 (3) ◽  
pp. 133-140 ◽  
Author(s):  
Jing Pan ◽  
Fei Zhao ◽  
Guifang Zhang ◽  
Yu Pan ◽  
Lijun Sun ◽  
...  
Keyword(s):  
De Novo ◽  
Leaf Explants ◽  
Developmental Status ◽  
Root Regeneration ◽  
Regeneration Efficiency

scholarly journals ABI3 plays a role in de-novo root regeneration from Arabidopsis thaliana callus cells

2020 ◽  
Vol 15 (9) ◽  
pp. 1794147
Author(s):  
Sourabh Sengupta ◽  
Ronita Nag Chaudhuri
Keyword(s):  
Arabidopsis Thaliana ◽  
De Novo ◽  
Root Regeneration

scholarly journals ETHYLENE INSENSITIVE 3 suppresses plant de novo root regeneration from leaf explants and mediates age-regulated regeneration decline

Development ◽  
2020 ◽  
Vol 147 (9) ◽  
pp. dev179457 ◽  
Author(s):  
Hong Li ◽  
Lulu Yao ◽  
Lili Sun ◽  
Ziqiang Zhu
Keyword(s):  
De Novo ◽  
Leaf Explants ◽  
Root Regeneration

scholarly journals An auxin-mediated ultradian rhythm promotes root regeneration in Arabidopsis

2021 ◽  
Author(s):  
Quy Vu ◽  
Kitae Song ◽  
Sung-Jin Park ◽  
Lin Xu ◽  
Hong Gil Nam ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
De Novo ◽  
Developmental Process ◽  
Ultradian Rhythm ◽  
Large Set ◽  
Biological Processes ◽  
Failure To Rescue ◽  
Genetic Studies ◽  
Root Regeneration ◽  
Short Period

Abstract Ultradian rhythms have been proved to be critical for diverse biological processes. However, comprehensive understanding of the short-period rhythms remains limited. Here, we discover that leaf excision triggers a gene expression rhythm with ~ 3-h periodicity, named as the excision ultradian rhythm (UR), which is regulated by the plant hormone auxin. Transcriptome analysis found more than 4,000 excision UR genes which are diverse in terms of biological function. Promoter–luciferase analyses showed that the spatiotemporal patterns of the excision UR were positively associated with de novo root regeneration (DNRR), a post-embryonic developmental process. Genetic studies showed that EXCISION ULTRADIAN RHYTHM 1 (EUR1), which encodes ENHANCER OF ABSCISIC ACID CO-RECEPTOR1 (EAR1), an abscisic acid signaling regulator, was required to generate the excision ultradian rhythm and promote root regeneration. Moreover, the ear1 mutant exhibited the absence of auxin-induced excision UR generation and partial failure to rescue DNRR. These results demonstrate that leaf excision activates EAR1-mediated excision UR and reprograms the expression of a large set of genes involved in DNRR.

A complex regulatory network underlies de novo root regeneration in red maple (Acer rubrum)

Botany ◽  
2021 ◽  
Author(s):  
Mengyi Li ◽  
Yunhe Cheng ◽  
Yanlin Li ◽  
Dexin Gan ◽  
Xiaoying Yu
Keyword(s):  
Regulatory Network ◽  
De Novo ◽  
Cell Formation ◽  
Acer Rubrum ◽  
Auxin Signaling ◽  
Rna Seq ◽  
Red Maple ◽  
Root Regeneration ◽  
Complex Regulatory Network ◽  
Root Primordium

Red maple (Acer rubrum L.) is ornamentally and medicinally valuable. However, its wide application is restricted by the difficulty of rooting in cuttings. We analyzed paraffin sections of roots regenerating using RNA-Seq to decipher the mechanisms underlying de novo root regeneration (DNRR) in red maple cuttings. This work contributes to improving the rooting rate and shortening the rooting time. We identified four stages during DNRR: 0 day after induction (DAI), no new cell formation; 30 DAI, root meristem organization; 36 DAI, root primordium formation; and 45 DAI, root elongation growth. We identified 37,959 unigenes by de novo assembly, with 25,477(67.12%) functionally annotated. Furthermore, we identified 1,285 differentially expressed genes (DEGs) between adjacent stages. From GO and KEGG enrichment networks, we found evidence that plant hormones are significant in DNRR of red maple cuttings. Specifically, 149 DEGs functioned in hormone signal transduction pathways, particularly those involving ethylene, auxin, and jasmonic acid (JA). We propose a complex regulatory network model of DNRR in red maple, where wounding induces root regeneration through pathways of JA and auxin signaling. The transcription factors ERF109 and ERF115 integrate JA signal and participate in DNRR directly by regulating SCR activation and indirectly, by promoting auxin biosynthesis.

The Molecular Basis of Age-Modulated Plant De Novo Root Regeneration Decline in Arabidopsis thaliana

2020 ◽  
Author(s):  
Lili Sun ◽  
Ziqiang Zhu
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Fate ◽  
Adventitious Roots ◽  
De Novo ◽  
Leaf Explants ◽  
Auxin Biosynthesis ◽  
Regeneration Capacity ◽  
Exogenous Hormone ◽  
Root Regeneration ◽  
Wox Genes

Abstract Plants possess a regeneration capacity that enables them to survive after wounding. For example, detached Arabidopsis thaliana leaves are able to form adventitious roots from their cutting sites even in the absence of exogenous hormone supplements, as process termed de novo root regeneration (DNRR). Wounding rapidly induces auxin biosynthesis at the cutting sites and then elicits a signaling cascade to promote cell fate transitions and finally generate the adventitious roots. However, rooting rates in older plants are much lower than in younger leaf explants. In this review, we highlight the recent breakthroughs in the understanding of DNRR decay in older plants from at least two independent signaling routes: (i) via the accumulation of EIN3 protein in older plants, which directly suppresses expression of WUSCHEL RELATED HOMEOBOX (WOX) genes to inhibit rooting; (ii) the miR156-SPLs-AP2/ERFs pathway, which modulates root regeneration by reducing auxin biosynthesis.

scholarly journals Red light controls adventitious root regeneration by modulating hormone homeostasis in Picea abies seedlings

2020 ◽  
Author(s):  
Sanaria Alallaq ◽  
Alok Ranjan ◽  
Federica Brunoni ◽  
Ondřej Novák ◽  
Abdellah Lakehal ◽  
...  
Keyword(s):  
Norway Spruce ◽  
Adventitious Root ◽  
De Novo ◽  
Red Light ◽  
Cellular Level ◽  
Promoting Effect ◽  
Endogenous Factors ◽  
Root Regeneration ◽  
Negative Effect ◽  
Hormone Homeostasis

AbstractVegetative propagation relies on the capacity of plants to regenerate de novo adventitious roots (ARs), a quantitative trait controlled by the interaction of endogenous factors such as hormones and environmental cues among which light plays a central role. However, the physiological and molecular components mediating light cues during AR initiation (ARI) remain largely elusive. We explored the effect of light spectral quality on ARI in de-rooted Norway spruce seedlings as well as on hormone metabolism with sensitive mass spectrometry-based methods. We coupled this to gene expression analysis to identify potential signaling pathways and to extensive anatomical characterization to investigate ARI at the cellular level. We showed that in contrast to white light and blue light, red light promoted ARI likely by reducing jasmonate (JA) and JA-isoleucine biosynthesis and repressing the accumulation of isopentyl-adenine-type cytokinins and abscisic acid. We confirmed that exogenously applied JA and/or CK inhibit ARI, and found that they possibly act in the same pathway. The negative effect of JA was confirmed at the histological level. We showed that JA represses the early events of ARI. In conclusion, RL promotes ARI by repressing the accumulation of the wound-induced phytohormones JA and CK.HighlightBlue and red light have an opposite effect on adventitious root initiation in Norway spruce hypocotyl, red light having a promoting effect by modulating hormone homeostasis.

scholarly journals Regulation of touch dependant de novo root regeneration in Arabidopsis

2020 ◽  
Author(s):  
Anju Pallipurath Shanmukhan ◽  
Mabel Maria Mathew ◽  
Mohammed Aiyaz ◽  
Abdul Kareem ◽  
Dhanya Radhakrishnan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Transcription Factor ◽  
De Novo ◽  
Callus Formation ◽  
Detached Leaf ◽  
Root Regeneration ◽  
Regulatory Module ◽  
Experimental Approaches ◽  
Frame Work ◽  
Regeneration Capability

AbstractThe versatile regeneration capability of leaves enable even a detached Arabidopsis leaf to yield two kinds of regenerative responses namely, wound healing at the cut end in form of callus formation or de novo root regeneration (DNRR). Using various experimental approaches, we show that the factor favouring DNRR over callus formation seems to be a mechanical cue, possibly touch, at the cut end of the detached leaf. Here, we show that the forced expression of a PLETHORA transcription factor can bypass the need for touch to initiate DNRR. Our findings provide a genetic frame-work for touch dependant DNRR and suggest that a core PLT transcription regulatory module acts in response to mechano-sensing stimuli.

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