A Histochemical Study of Fat Absorption in the Small Intestine of the Rat

1954 ◽  
Vol s3-95 (30) ◽  
pp. 153-158
Author(s):  
W. HEWITT
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Mucous Membrane ◽  
Histochemical Study ◽  
Fat Absorption ◽  
Red Coloration

Experiments are described in which sections from the small intestine of rats, fed on triolein 2¾ hours previously, were examined with Sudan black and the acid haematein test, with and without Sudan red coloration. The histochemical appearances in the epithelial cells of the mucous membrane are described. Lipoid can be observed not only within the epithelial cells and their free border but also as particles between the cells.

Further Observations on the Histochemistry of Fat Absorption in the Small Intestine of the Rat

1956 ◽  
Vol s3-97 (38) ◽  
pp. 199-203
Author(s):  
W. HEWITT
Keyword(s):  
Small Intestine ◽  
Fatty Acid ◽  
Epithelial Cells ◽  
Mucous Membrane ◽  
Direct Relationship ◽  
Fat Absorption ◽  
Previous Description ◽  
Histochemical Tests

Studies have been made of the small intestine of rats previously fed with triolein. A previous description of the location of the lipid and phospholipid has been confirmed by using Sudan black and the acid haematein test. In addition histochemical tests have been applied to detect neutral fat and fatty acid. The lipid in the free border of the epithelial cells of the mucous membrane consisted of fatty acid and probably neutral fat, which would support the belief that when neutral fat is absorbed some of it is unhydrolysed. Additional evidence for this is provided by the fact that neutral fat could also be detected between the lateral parts of the cells. Occasionally fatty acid was also detectable in this location. Variable proportions of neutral fat, fatty acid, and phospholipid were to be found inside the epithelial cells and it appeared possible that there was a direct relationship between the amounts of neutral fat and phospholipid present.

scholarly journals V. Research on the minute anatomy of the alimentary canal

1876 ◽  
Vol 24 (164-170) ◽  
pp. 241-244 ◽  
Keyword(s):  
Small Intestine ◽  
Mucous Membrane ◽  
Alimentary Canal ◽  
Fat Absorption ◽  
History Of

This research was commenced and carried on for the first fifteen months under the direction of Dr. Klein; during the last year it was continued independently. continued independently. Chapter I. describes the minute anatomy, of the mucous membrane of she small intestine and the method of fat-absorption. The author commences the Chapter by giving a history of the researches of previous observers, and the methods he employed for hardening and staining the tissue. He then gives a description of the minute structure of the mucosa.

The Influence of Radiotherapy on IL-2 and IL-6 Secretions of Mucous Membrane Epithelial Cells of Wistar Small Intestine

2014 ◽  
Vol 71 (1) ◽  
pp. 35-38
Author(s):  
Bin Liu ◽  
Xiaoling Li ◽  
Fulu Ai ◽  
Tianlu Wang ◽  
Yun Chen ◽  
...  
Keyword(s):  
Small Intestine ◽  
Epithelial Cells ◽  
Mucous Membrane

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

The protease phenotype and crystalline nature of the granules of intraepithelial mast cells in Trichinella spiralis-infected mice

1995 ◽  
Vol 53 ◽  
pp. 818-819
Author(s):  
D.S. Friend ◽  
N. Ghildyal ◽  
M.F. Gurish ◽  
K.F. Austen ◽  
R.L. Stevens
Keyword(s):  
Small Intestine ◽  
Mast Cells ◽  
Epithelial Cells ◽  
Lamina Propria ◽  
Trichinella Spiralis ◽  
Intestinal Epithelial ◽  
Carboxypeptidase A ◽  
C3h Mice ◽  
Granule Morphology ◽  
Crystalline Nature

Trichinella spiralis induces a profound mastocytosis and eosinophilia in the small intestine of the infected mouse. Mouse mast cells (MC) store in their granules various combinations of at least five chymotryptic chymases [designated mouse MC protease (mMCP) 1 to 5], two tryptic proteases designated mMCP-6 and mMCP-7 and an exopeptidase, carboxypeptidase A (mMC-CPA). Using antipeptide, protease -specific antibodies to these MC granule proteases, immunohistochemistry was done to determine the distribution, number and protease phenotype of the MCs in the small intestine and spleen 10 to >60 days after Trichinella infection of BALB/c and C3H mice. TEM was performed to evaluate the granule morphology of the MCs between intestinal epithelial cells and in the lamina propria (mucosal MCs) and in the submucosa, muscle and serosa of the intestine (submucosal MCs).As noted in the table below, the number of submucosal MCs remained constant throughout the study. In contrast, on day 14, the number of MCs in the mucosa increased ~25 fold. Increased numbers of MCs were observed between epithelial cells in the mucosal crypts, in the lamina propria and to a lesser extent, between epithelial cells of the intestinal villi.

Sign in / Sign up

Export Citation Format

Share Document