The Cytoplasmic Basophilia (Ribonucleic Acid) in Epithelial Cells of the Mucous Membrane of Human Small Intestine and the Gland of BRUNNER

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

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Structures of blood group glycosphingolipids of human small intestine. A relation between the expression of fucolipids of epithelial cells and the ABO, Le and Se phenotype of the donor.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)48309-3 ◽

1987 ◽

Vol 262 (14) ◽

pp. 6758-6765 ◽

Cited By ~ 4

Author(s):

S Björk ◽

M E Breimer ◽

G C Hansson ◽

K A Karlsson ◽

H Leffler

Keyword(s):

Small Intestine ◽

Epithelial Cells ◽

Blood Group ◽

Human Small Intestine

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The Fate of Effete Epithelial Cells at the Villus Tips of the Human Small Intestine.

Archives of Histology and Cytology ◽

10.1679/aohc.58.205 ◽

1995 ◽

Vol 58 (2) ◽

pp. 205-219 ◽

Cited By ~ 65

Author(s):

Takeshi SHIBAHARA ◽

Noboru SATO ◽

Satoshi WAGURI ◽

Toshihiko IWANAGA ◽

Akira NAKAHARA ◽

...

Keyword(s):

Small Intestine ◽

Epithelial Cells ◽

Human Small Intestine

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A Histochemical Study of Fat Absorption in the Small Intestine of the Rat

Journal of Cell Science ◽

10.1242/jcs.s3-95.30.153 ◽

1954 ◽

Vol s3-95 (30) ◽

pp. 153-158

Author(s):

W. HEWITT

Keyword(s):

Small Intestine ◽

Epithelial Cells ◽

Mucous Membrane ◽

Histochemical Study ◽

Fat Absorption ◽

Red Coloration

Experiments are described in which sections from the small intestine of rats, fed on triolein 2¾ hours previously, were examined with Sudan black and the acid haematein test, with and without Sudan red coloration. The histochemical appearances in the epithelial cells of the mucous membrane are described. Lipoid can be observed not only within the epithelial cells and their free border but also as particles between the cells.

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Further Observations on the Histochemistry of Fat Absorption in the Small Intestine of the Rat

Journal of Cell Science ◽

10.1242/jcs.s3-97.38.199 ◽

1956 ◽

Vol s3-97 (38) ◽

pp. 199-203

Author(s):

W. HEWITT

Keyword(s):

Small Intestine ◽

Fatty Acid ◽

Epithelial Cells ◽

Mucous Membrane ◽

Direct Relationship ◽

Fat Absorption ◽

Previous Description ◽

Histochemical Tests

Studies have been made of the small intestine of rats previously fed with triolein. A previous description of the location of the lipid and phospholipid has been confirmed by using Sudan black and the acid haematein test. In addition histochemical tests have been applied to detect neutral fat and fatty acid. The lipid in the free border of the epithelial cells of the mucous membrane consisted of fatty acid and probably neutral fat, which would support the belief that when neutral fat is absorbed some of it is unhydrolysed. Additional evidence for this is provided by the fact that neutral fat could also be detected between the lateral parts of the cells. Occasionally fatty acid was also detectable in this location. Variable proportions of neutral fat, fatty acid, and phospholipid were to be found inside the epithelial cells and it appeared possible that there was a direct relationship between the amounts of neutral fat and phospholipid present.

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