Methylene Blue Sensitized Degradation of Sodium Hyaluronate through Photoinduced Electron Transfer

2000 ◽  
Vol 29 (4) ◽  
pp. 354-355
Author(s):  
Masanobu Kojima ◽  
Tomoko Takagi ◽  
Chie Matsubara ◽  
Kimitoshi Nakamura
Keyword(s):  
Methylene Blue ◽  
Electron Transfer ◽  
Photoinduced Electron Transfer ◽  
Sodium Hyaluronate

Semiconducting Polymer Dot-Based Ratiometric Fluorescence Nanoprobe for DNA Detection

2021 ◽  
Vol 21 (11) ◽  
pp. 5776-5783
Author(s):  
Zhen-Hu Gong ◽  
Zong-Nan Wei ◽  
Yi-Zhang Liu ◽  
Lu-Fei Xiao
Keyword(s):  
Methylene Blue ◽  
Electron Transfer ◽  
Photoinduced Electron Transfer ◽  
Deoxyribonucleic Acid ◽  
Experimental Conditions ◽  
Semiconducting Polymer ◽  
Electron Transfer Mechanism ◽  
Fluorescence Nanoprobe ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

A ratiometric fluorescent deoxyribonucleic acid probe was synthesized using the photoinduced electron transfer mechanism, involving the interactions between different fluorescent components and electron acceptors. Double-emission carboxyl functionalized semiconducting polymer dots were synthesized using the nanoprecipitation method and applied as the flurophore, while methylene blue was used as the electron acceptor. Photoinduced electron transfer between different polymer dots components and methylene blue can achieve ratiometric modulation of the overall fluorescence in the system. The addition of deoxyribonucleic acid restores the fluorescence intensity, because the stronger interactions between deoxyribonucleic acid and methylene blue results in methylene blue being separated from the polymer dots. Under the optimized experimental conditions, system fluorescence was restored to its maximum when the concentration of deoxyribonucleic acid reached 200 nM, the linear range was 0.006-200 nM (R2 = 0.995). This probe was reasonably free from interference, showing a good response to deoxyribonucleic acid, with strong application data from actual samples.

Photoinduced Electron Transfer in Dry Gelatin Containing Bound Methylene Blue

1998 ◽  
Vol 67 (1) ◽  
pp. 101-105 ◽  
Author(s):  
Gerald J. Smith ◽  
Anthony Harriman ◽  
Anthony D. Woolhouse ◽  
Timothy G. Haskell ◽  
Thomas H. Barnes
Keyword(s):  
Methylene Blue ◽  
Electron Transfer ◽  
Photoinduced Electron Transfer

Computationally Assisted Design of High Signal-to-Noise Photoinduced Electron Transfer-Based Voltage-Sensitive Dyes

2020 ◽  
Author(s):  
Rishikesh Kulkarni ◽  
Anneliese Gest ◽  
Chun Kei Lam ◽  
Benjamin Raliski ◽  
Feroz James ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Dft Calculations ◽  
Photoinduced Electron Transfer ◽  
Density Functional ◽  
Embryonic Stem ◽  
High Sensitivity ◽  
Md Simulations ◽  
High Signal ◽  
Signal To Noise ◽  
Green Fluorescent

<p>High signal-to-noise optical voltage indicators will enable simultaneous interrogation of membrane potential in large ensembles of neurons. However, design principles for voltage sensors with high sensitivity and brightness remain elusive, limiting the applicability of voltage imaging. In this paper, we use molecular dynamics (MD) simulations and density functional theory (DFT) calculations to guide the design of a bright and sensitive green-fluorescent voltage-sensitive fluorophore, or VoltageFluor (VF dye), that uses photoinduced electron transfer (PeT) as a voltage-sensing mechanism. MD simulations predict an 11% increase in sensitivity due to membrane orientation, while DFT calculations predict an increase in fluorescence quantum yield, but a decrease in sensitivity due to a decrease in rate of PeT. We confirm these predictions by synthesizing a new VF dye and demonstrating that it displays the expected improvements by doubling the brightness and retaining similar sensitivity to prior VF dyes. Combining theoretical predictions and experimental validation has resulted in the synthesis of the highest signal-to-noise green VF dye to date. We use this new voltage indicator to monitor the electrophysiological maturation of human embryonic stem cell-derived medium spiny neurons. </p>

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