scholarly journals Polarity-dependent Photophysical Properties of Hemicyanine Dyes and Their Application in 2-Photon Microscopy Biological Imaging

2012 ◽  
Vol 41 (5) ◽  
pp. 528-530 ◽  
Author(s):  
Yuta Kimura ◽  
Atsuya Momotake ◽  
Noriko Takahashi ◽  
Haruo Kasai ◽  
Tatsuo Arai
2004 ◽  
Vol 44 (3) ◽  
pp. 708
Author(s):  
Kim D. ◽  
Shim T. K. ◽  
Lee M. H. ◽  
Sung J. ◽  
Rhee B. K. ◽  
...  

2008 ◽  
Vol 112 (7) ◽  
pp. 1906-1912 ◽  
Author(s):  
Taekyu Shim ◽  
Myoung Hee Lee ◽  
Doseok Kim ◽  
Yukio Ouchi

2021 ◽  
Author(s):  
Le Liang ◽  
Peng Zheng ◽  
Sisi Jia ◽  
Krishanu Ray ◽  
Yun Chen ◽  
...  

Nitrogen-vacancy (NV) centers in diamonds are promising solid-state quantum emitters for developing superior biological imaging modalities. They possess desired bio-compatibility, photostability and electronic spin-related photophysical properties that are optically accessible at room temperature. Yet, bare nanodiamond-based imaging modalities are limited by the brightness and temporal resolution due to the intrinsically long lifetime of NV centers. Moreover, it remains a technological challenge using top-down fabrication to create freestanding hybrid nanodiamond imaging probes with enhanced performance. In this study, we leverage the bottom-up DNA self-assembly to develop a hybrid plasmonic nanodiamond construct, which we coin as the plasmon-enhanced nanodiamond (PEN), for biological imaging. The PEN nano-assembly features a closed plasmonic nanocavity that completely encapsulates a single nanodiamond, thus enabling the largest possible plasmonic enhancement to accelerate the emission dynamics of NV centers. Creation of the PEN nano-assembly is size-independent, so is its broadband scattering spectrum that is optimally overlapped with the emission spectrum of NV centers. Study of the structure-property correlation reveals that the optimal condition for emission dynamics modification is causally linked to that for a plasmonic nanocavity. The cellular internalization and cytotoxicity studies further confirm the delivery efficiency and biological safety of PEN nano-assemblies. Collectively, the PEN nano-assembly provides a promising approach for manipulating photophysical properties of solid-state quantum emitters and could serve as a versatile platform to uncover non-trivial quantum effects in biological systems.


2008 ◽  
Vol 76 (1) ◽  
pp. 118-124 ◽  
Author(s):  
Du-Xia Cao ◽  
Zhi-Qiang Liu ◽  
Guo-Hui Zhang ◽  
Feng-Xia Cao ◽  
Hong-Yu Chen ◽  
...  

2000 ◽  
Vol 104 (7) ◽  
pp. 1388-1392 ◽  
Author(s):  
Jiho Kim ◽  
Minyung Lee ◽  
Jae-Hun Yang ◽  
Jin-Ho Choy

2003 ◽  
Vol 58 (1) ◽  
pp. 47-58 ◽  
Author(s):  
Beata Jędrzejewska ◽  
Janina Kabatc ◽  
Marek Pietrzak ◽  
Jerzy Pa̧czkowski

Author(s):  
Steve Lindaas ◽  
Chris Jacobsen ◽  
Alex Kalinovsky ◽  
Malcolm Howells

Soft x-ray microscopy offers an approach to transmission imaging of wet, micron-thick biological objects at a resolution superior to that of optical microscopes and with less specimen preparation/manipulation than electron microscopes. Gabor holography has unique characteristics which make it particularly well suited for certain investigations: it requires no prefocussing, it is compatible with flash x-ray sources, and it is able to use the whole footprint of multimode sources. Our method serves to refine this technique in anticipation of the development of suitable flash sources (such as x-ray lasers) and to develop cryo capabilities with which to reduce specimen damage. Our primary emphasis has been on biological imaging so we use x-rays in the water window (between the Oxygen-K and Carbon-K absorption edges) with which we record holograms in vacuum or in air.The hologram is recorded on a high resolution recording medium; our work employs the photoresist poly(methylmethacrylate) (PMMA). Following resist “development” (solvent etching), a surface relief pattern is produced which an atomic force microscope is aptly suited to image.


Author(s):  
K. N. Colonna ◽  
G. Oliphant

Harmonious use of Z-contrast imaging and digital image processing as an analytical imaging tool was developed and demonstrated in studying the elemental constitution of human and maturing rabbit spermatozoa. Due to its analog origin (Fig. 1), the Z-contrast image offers information unique to the science of biological imaging. Despite the information and distinct advantages it offers, the potential of Z-contrast imaging is extremely limited without the application of techniques of digital image processing. For the first time in biological imaging, this study demonstrates the tremendous potential involved in the complementary use of Z-contrast imaging and digital image processing.Imaging in the Z-contrast mode is powerful for three distinct reasons, the first of which involves tissue preparation. It affords biologists the opportunity to visualize biological tissue without the use of heavy metal fixatives and stains. For years biologists have used heavy metal components to compensate for the limited electron scattering properties of biological tissue.


Sign in / Sign up

Export Citation Format

Share Document