scholarly journals Knock-in of labeled proteins into 5’UTR enables highly efficient generation of stable cell lines

2021 ◽  
Author(s):  
Faryal Ijaz ◽  
Koji Ikegami
Keyword(s):  
Cell Lines ◽  
Stable Cell Lines ◽  
Highly Efficient ◽  
Efficient Generation

scholarly journals Highly efficient generation of glutamatergic/cholinergic NT2-derived postmitotic human neurons by short-term treatment with the nucleoside analogue cytosine β- d -arabinofuranoside

2016 ◽  
Vol 16 (2) ◽  
pp. 541-551 ◽  
Author(s):  
Imanol González-Burguera ◽  
Ana Ricobaraza ◽  
Xabier Aretxabala ◽  
Sergio Barrondo ◽  
Gontzal García del Caño ◽  
...  
Keyword(s):  
Nucleoside Analogue ◽  
Term Treatment ◽  
Short Term ◽  
Short Term Treatment ◽  
Highly Efficient ◽  
Efficient Generation ◽  
Human Neurons

The role of the membrane-spanning domain and stalk region of N-acetylglucosaminyltransferase I in retention, kin recognition and structural maintenance of the Golgi apparatus in HeLa cells

1996 ◽  
Vol 109 (7) ◽  
pp. 1975-1989 ◽  
Author(s):  
T. Nilsson ◽  
C. Rabouille ◽  
N. Hui ◽  
R. Watson ◽  
G. Warren
Keyword(s):  
Golgi Apparatus ◽  
Cell Lines ◽  
Hela Cells ◽  
Kin Recognition ◽  
Dramatic Effect ◽  
Golgi Stack ◽  
Stable Cell Lines ◽  
Membrane Spanning ◽  
Necessary And Sufficient

Using a series of chimeric and truncated N-acetylglucosaminyltransferase I (NAGT I) molecules we have shown that part of the lumenal stalk region is both necessary and sufficient for kin recognition of mannosidase II and retention in the Golgi stack. The membrane-spanning domain was not required for retention, but replacing part or all of this domain with leucine residues did have a dramatic effect on Golgi morphology. In stable cell lines, stacked cisternae were replaced by tubulo-vesicular clusters containing the mutated NAGT I. The loss of stacked cisternae was proportional to the number of leucines used to replace the membrane-spanning domain.

scholarly journals Robust Production of Infectious Hepatitis C Virus (HCV) from Stably HCV cDNA-Transfected Human Hepatoma Cells

2005 ◽  
Vol 79 (22) ◽  
pp. 13963-13973 ◽  
Author(s):  
Zhaohui Cai ◽  
Chen Zhang ◽  
Kyung-Soo Chang ◽  
Jieyun Jiang ◽  
Byung-Chul Ahn ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Cell Lines ◽  
Culture System ◽  
Infectious Virus ◽  
Hcv Infection ◽  
Hcv Rna ◽  
Human Hepatoma ◽  
Stable Cell Lines ◽  
Increased Risk

ABSTRACT Hepatitis C virus (HCV) chronically infects approximately 170 million people worldwide, with an increased risk of developing cirrhosis and hepatocellular carcinoma. The study of HCV replication and pathogenesis has been hampered by the lack of an efficient stable cell culture system and small-animal models of HCV infection and propagation. In an effort to develop a robust HCV infection system, we constructed stable human hepatoma cell lines that contain a chromosomally integrated genotype 2a HCV cDNA and constitutively produce infectious virus. Transcriptional expression of the full-length HCV RNA genome is under the control of a cellular Pol II polymerase promoter at the 5′ end and a hepatitis delta virus ribozyme at the 3′ end. The resulting HCV RNA was expressed and replicated efficiently, as shown by the presence of high levels of HCV proteins as well as both positive- and negative-strand RNAs in the stable Huh7 cell lines. Stable cell lines robustly produce HCV virions with up to 108 copies of HCV viral RNA per milliliter (ml) of the culture medium. Subsequent infection of naïve Huh7.5 cells with HCV released from the stable cell lines resulted in high levels of HCV proteins and RNAs. Additionally, HCV infection was inhibited by monoclonal antibodies specific to CD81 and the HCV envelope glycoproteins E1 and E2, and HCV replication was suppressed by alpha interferon. Collectively, these results demonstrate the establishment of a stable HCV culture system that robustly produces infectious virus, which will allow the study of each aspect of the entire HCV life cycle.

Development of stable cell lines for production or regulated expression using matrix attachment regions

2001 ◽  
Vol 87 (1) ◽  
pp. 29-42 ◽  
Author(s):  
Monique Zahn-Zabal ◽  
Michel Kobr ◽  
Pierre-Alain Girod ◽  
Markus Imhof ◽  
Philippe Chatellard ◽  
...  
Keyword(s):  
Cell Lines ◽  
Matrix Attachment Regions ◽  
Stable Cell Lines ◽  
Regulated Expression
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